Explanipedia

Development and validation of AI/ML derived splice-switching oligonucleotides Open

Alyssa D Fronk, Miguel Manzanares, Paulina Zheng, Adam Geier, Kendall Anderson , et al. · 2024

Splice-switching oligonucleotides (SSOs) are antisense compounds that act directly on pre-mRNA to modulate alternative splicing (AS). This study demonstrates the value that artificial intelligence/machine learning (AI/ML) provides for the …

SpliceIO™ a novel AI platform for the discovery of splicing-derived immunotherapeutic targets Open

M. Manzanares, Hasan E. Zümrüt, SUCHITA GERA, A. Casill, K. Anderson , et al. · 2022

An <i>In Vitro</i> Selection Platform to Identify Multiple Aptamers against Multiple Cell-Surface Markers Using Ligand-Guided Selection Open

Nicole Williams, Sana Batool, Hasan E. Zümrüt, Rutika Patel, German Sosa , et al. · 2022

Aptamer ligand discovery against multiple molecules expressed on whole cells is an essential component in molecular tool development. However, owing to their intrinsic structural characteristics, cell-surface receptors have proven to be ch…

Ligand-Guided Selection with Artificially Expanded Genetic Information Systems against TCR-CD3ε Open

Hasan E. Zümrüt, Zunyi Yang, Nicole Williams, Joekeem D. Arizala, Sana Batool , et al. · 2019

Here we are reporting, for the first time, a ligand-guided selection (LIGS) experiment using an artificially expanded genetic information system (AEGIS) to successfully identify an AEGIS-DNA aptamer against T cell receptor-CD3ε expressed o…

Ligand Guided Selection (LIGS) of Artificial Nucleic Acid Ligands against Cell Surface Targets Open

Hasan E. Zümrüt, Prabodhika Mallikaratchy · 2019

With the success of RNA-based therapeutic drugs, the demand has increased for sophisticated nucleic-acid-based targeting agents. Nucleic acid aptamers (NAAs), in this regard, represent a suitable class of molecules with synthetic versatili…

Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands Open

Hasan E. Zümrüt, Sana Batool, Kimon V. Argyropoulos, Nicole Williams, Roksana Azad , et al. · 2019

To discover DNA ligands against a predetermined receptor protein complex, we introduce a comprehensive version of ligand-guided selection (LIGS). LIGS is, itself, a variant of systematic evolution of ligands by exponential enrichment (SELE…

The role of G-quadruplex structures of LIGS-generated aptamers R1.2 and R1.3 in IgM specific recognition Open

Federica Moccia, Chiara Platella, Domenica Musumeci, Sana Batool, Hasan E. Zümrüt , et al. · 2019

Engineered Aptamers to Probe Molecular Interactions on the Cell Surface Open

Sana Batool, Sanam Bhandari, Shanell George, Precious Okeoma, Nabeela A. Van , et al. · 2017

Significant progress has been made in understanding the nature of molecular interactions on the cell membrane. To decipher such interactions, molecular scaffolds can be engineered as a tool to modulate these events as they occur on the cel…

Structural optimization of an aptamer generated from Ligand-Guided Selection (LIGS) resulted in high affinity variant toward mIgM expressed on Burkitt's lymphoma cell lines Open

Hasan E. Zümrüt, Sana Batool, Nabeela A. Van, Shanell George, Sanam Bhandari , et al. · 2017

Ligand-guided selection of aptamers against T-cell Receptor-cluster of differentiation 3 (TCR-CD3) expressed on Jurkat.E6 cells Open

Hasan E. Zümrüt, Mst. Naznin Ara, George Maio, Nabeela A. Van, Sana Batool , et al. · 2016

Ligand-Guided Selection of Target-Specific Aptamers: A Screening Technology for Identifying Specific Aptamers Against Cell-Surface Proteins Open

Hasan E. Zümrüt, Mst. Naznin Ara, María Fraile, George Maio, Prabodhika Mallikaratchy · 2016

We report on a new strategy for identifying highly specific aptamers against a predetermined epitope of a target. Termed âligand-guided selectionâ (LIGS), this method uniquely exploits the selection step, the core of SELEX (Systematic …

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