Heather E. Findlay
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View article: Methods to study folding of alpha-helical membrane proteins in lipids
Methods to study folding of alpha-helical membrane proteins in lipids Open
How alpha-helical membrane proteins fold correctly in the highly hydrophobic membrane interior is not well understood. Their folding is known to be highly influenced by the lipids within the surrounding bilayer, but the majority of folding…
View article: Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein
Cell-Free Expression to Probe Co-Translational Insertion of an Alpha Helical Membrane Protein Open
The majority of alpha helical membrane proteins fold co-translationally during their synthesis on the ribosome. In contrast, most mechanistic folding studies address refolding of full-length proteins from artificially induced denatured sta…
View article: Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter
Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter Open
Proton-coupled transporters use transmembrane proton gradients to power active transport of nutrients inside the cell. High-resolution structures often fail to capture the coupling between proton and ligand binding, and conformational chan…
View article: Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies
Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies Open
Co-translational folding studies of membrane proteins lag behind cytosolic protein investigations largely due to the technical difficulty in maintaining membrane lipid environments for correct protein folding. Stalled ribosome-bound nascen…
View article: Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB
Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB Open
Resistance-nodulation-division (RND) efflux pumps play a key role in inherent and evolved multidrug-resistance (MDR) in bacteria. AcrB is the prototypical member of the RND family and acts to recognise and export a wide range of chemically…
View article: Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter
Hydrogen-deuterium exchange mass spectrometry captures distinct dynamics upon substrate and inhibitor binding to a transporter Open
Proton-coupled transporters use transmembrane proton gradients to power active transport of nutrients inside the cell. High-resolution structures often fail to capture the coupling between proton and ligand binding, and conformational chan…
View article: Probing membrane protein properties using droplet interface bilayers
Probing membrane protein properties using droplet interface bilayers Open
Integral membrane proteins comprise a large proportion of drug targets, yet are challenging to study in vitro due to their amphiphilic nature. Conducting useful functional in vitro studies requires an artificial membrane that can mimic the…
View article: Lipids modulate the insertion and folding of the nascent chains of alpha helical membrane proteins
Lipids modulate the insertion and folding of the nascent chains of alpha helical membrane proteins Open
Membrane proteins must be inserted into a membrane and folded into their correct structure to function correctly. This insertion occurs during translation and synthesis by the ribosome for most α-helical membrane proteins. Precisely how th…
View article: Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles
Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles Open
Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations an…
View article: Lipid bilayer composition modulates the unfolding free energy of a knotted α-helical membrane protein
Lipid bilayer composition modulates the unfolding free energy of a knotted α-helical membrane protein Open
Significance Cells in our bodies sense and communicate with the outside world via proteins embedded in membranes that surround the cells. As with all proteins, a fundamental parameter governing their biological function is the inherent, th…
View article: Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions
Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions Open
The interplay between membrane proteins and the lipids of the membrane is important for cellular function, however, tools enabling the interrogation of protein dynamics within native lipid environments are scarce and often invasive. We sho…
View article: The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition
The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition Open
Lipids play key roles in Biology. Mechanical properties of the lipid bilayer influence their neighbouring membrane proteins, however it is unknown whether different membrane protein properties have the same dependence on membrane mechanics…
View article: Comparative stability of Major Facilitator Superfamily transport proteins
Comparative stability of Major Facilitator Superfamily transport proteins Open
Membrane transporters are a vital class of proteins for which there is little available structural and thermodynamic information. The Major Facilitator Superfamily (MFS) is a large group of transport proteins responsible for transporting a…
View article: In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers
In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers Open
Nature encapsulates reactions within membrane-bound compartments, affording sequential and spatial control over biochemical reactions. Droplet Interface Bilayers are evolving into a valuable platform to mimic this key biological feature in…