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View article: The comet assay as a tool in human biomonitoring studies: Effects of confounding factors
The comet assay as a tool in human biomonitoring studies: Effects of confounding factors Open
View article: Influence of pH and lysis duration on DNA damage detection: a comparison of neutral and alkaline comet assays
Influence of pH and lysis duration on DNA damage detection: a comparison of neutral and alkaline comet assays Open
The comet assay is a widely used method for measuring DNA damage and DNA repair. When DNA strand breaks happen, the supercoiling of DNA is relaxed, and after alkaline or neutral electrophoresis, depending on the type of performed comet ass…
View article: The comet assay as a tool in human biomonitoring of exposure to heavy metals – A systematic review and meta-analysis
The comet assay as a tool in human biomonitoring of exposure to heavy metals – A systematic review and meta-analysis Open
Exposure to heavy metals such as lead, arsenic and chromium is associated with genotoxicity and increased risk of cancer. In this systematic review and meta-analysis, we have assessed effects of heavy metal exposure on levels of DNA strand…
View article: A proposed screening strategy for evaluating the genotoxicity potential of botanicals and botanical extracts
A proposed screening strategy for evaluating the genotoxicity potential of botanicals and botanical extracts Open
Botanicals have long been used to promote health and treat diseases, but the safety of many currently marketed botanicals has not been adequately evaluated. Given the chemical complexity of botanicals, which often contain numerous unknown …
View article: Micronuclei as genotoxicity endpoint applied in the co-culture of two mammalian cell lines
Micronuclei as genotoxicity endpoint applied in the co-culture of two mammalian cell lines Open
There has been a shift from traditional animal models towards alternative methods. While 2D cell culture has a decade long tradition, more advances methods like 3D cultures, organoids, and co-culture techniques, which better mimic in vivo …
View article: Genotoxicity of selected cannabinoids in human lymphoblastoid TK6 cells
Genotoxicity of selected cannabinoids in human lymphoblastoid TK6 cells Open
Natural non-psychoactive cannabinoids such as cannabigerol (CBG), cannabidiol (CBD), cannabichromene (CBC), cannabidivarin (CBDV), and cannabinol (CBN) are increasingly consumed as constituents of dietary products because of the health ben…
View article: Obesity-associated non-oxidative genotoxic stress alters trophoblast turnover in human first-trimester placentas
Obesity-associated non-oxidative genotoxic stress alters trophoblast turnover in human first-trimester placentas Open
Placental growth is most rapid during the first trimester (FT) of pregnancy, making it vulnerable to metabolic and endocrine influences. Obesity, with its inflammatory and oxidative stress, can cause cellular damage. We hypothesized that m…
View article: Objectives and achievements of the HUMN project on its 26th anniversary
Objectives and achievements of the HUMN project on its 26th anniversary Open
View article: Disruptive effects of plasticizers bisphenol A, F, and S on steroidogenesis of adrenocortical cells
Disruptive effects of plasticizers bisphenol A, F, and S on steroidogenesis of adrenocortical cells Open
Introduction Endocrine disrupting chemicals (EDCs) are known to interfere with endocrine homeostasis. Their impact on the adrenal cortex and steroidogenesis has not yet been sufficiently elucidated. This applies in particular to the ubiqui…
View article: The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review
The Comet Assay as a Tool in Human Biomonitoring Studies of Environmental and Occupational Exposure to Chemicals—A Systematic Scoping Review Open
Biomonitoring of human populations exposed to chemical substances that can act as potential mutagens or carcinogens, may enable the detection of damage and early disease prevention. In recent years, the comet assay has become an important …
View article: Enhancing Chemical-Induced Human Carcinogenic Risk Evaluation through Advanced AI Technologies
Enhancing Chemical-Induced Human Carcinogenic Risk Evaluation through Advanced AI Technologies Open
Introduction: Efficient cancer risk assessment is vital for sustainable practices in pharma, agriculture, and environmental protection [...]
View article: Differential Modulation of Markers of Oxidative Stress and DNA Damage in Arterial Hypertension
Differential Modulation of Markers of Oxidative Stress and DNA Damage in Arterial Hypertension Open
Patients with arterial hypertension have an increased risk of developing tumors, particularly renal cell carcinoma. Arterial hypertension is linked to DNA damage via the generation of oxidative stress, in which an upregulated renin–angiote…
View article: Endocrine Disruptors: Focus on the Adrenal Cortex
Endocrine Disruptors: Focus on the Adrenal Cortex Open
Endocrine-disrupting chemicals (EDCs) are exogenous substances known to interfere with endocrine homeostasis and promote adverse health outcomes. Their impact on the adrenal cortex, corticosteroids and their physiological role in the organ…
View article: Datasets Construction and Development of QSAR Models for Predicting Micronucleus In Vitro and In Vivo Assay Outcomes
Datasets Construction and Development of QSAR Models for Predicting Micronucleus In Vitro and In Vivo Assay Outcomes Open
In silico (quantitative) structure–activity relationship modeling is an approach that provides a fast and cost-effective alternative to assess the genotoxic potential of chemicals. However, one of the limiting factors for model development…
View article: Inter-laboratory variation in measurement of DNA damage by the alkaline comet assay in the hCOMET ring trial
Inter-laboratory variation in measurement of DNA damage by the alkaline comet assay in the hCOMET ring trial Open
The comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the assay detects DNA migration from agarose gel-embedded nucleoids, which depends on assay conditions and the level of …
View article: Measuring DNA modifications with the comet assay: a compendium of protocols
Measuring DNA modifications with the comet assay: a compendium of protocols Open
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), a…
View article: Fate of micronuclei and micronucleated cells after treatment of HeLa cells with different genotoxic agents
Fate of micronuclei and micronucleated cells after treatment of HeLa cells with different genotoxic agents Open
View article: Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells
Genotoxicity of pyrrolizidine alkaloids in metabolically inactive human cervical cancer HeLa cells co-cultured with human hepatoma HepG2 cells Open
View article: Improving VAE based molecular representations for compound property prediction
Improving VAE based molecular representations for compound property prediction Open
View article: Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro
Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro Open
Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen spec…
View article: Do cytotoxicity and cell death cause false positive results in the in vitro comet assay?
Do cytotoxicity and cell death cause false positive results in the in vitro comet assay? Open
The comet assay is used to measure DNA damage induced by chemical and physical agents. High concentrations of test agents may cause cytotoxicity or cell death, which may give rise to false positive results in the comet assay. Systematic st…
View article: Obesity-related genomic instability and altered xenobiotic metabolism: possible consequences for cancer risk and chemotherapy
Obesity-related genomic instability and altered xenobiotic metabolism: possible consequences for cancer risk and chemotherapy Open
The increase in the prevalence of obesity has led to an elevated risk for several associated diseases including cancer. Several studies have investigated the DNA damage in human blood samples and showed a clear trend towards increased DNA …
View article: Cell survival after DNA damage in the comet assay
Cell survival after DNA damage in the comet assay Open
View article: Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
Author Correction: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death Open
View article: DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death Open
View article: “Micronuclei and Disease” special issue: Aims, scope, and synthesis of outcomes
“Micronuclei and Disease” special issue: Aims, scope, and synthesis of outcomes Open
View article: Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies
Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies Open
DNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyse the samples on the day of collection without any type of sto…
View article: Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay
Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay Open
The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified version…
View article: The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders Open
View article: Micronucleus frequency in buccal mucosa cells of patients with neurodegenerative diseases
Micronucleus frequency in buccal mucosa cells of patients with neurodegenerative diseases Open