Helmut Hanenberg
YOU?
Author Swipe
View article: <scp>SIRT</scp> 4 positively regulates autophagy via <scp>ULK</scp> 1, but independently of <scp>HDAC</scp> 6 and <scp>OPA</scp> 1
<span>SIRT</span> 4 positively regulates autophagy via <span>ULK</span> 1, but independently of <span>HDAC</span> 6 and <span>OPA</span> 1 Open
The sirtuin SIRT4 has been implicated in the control of autophagy and mitochondrial quality control via mitophagy. However, the role of SIRT4 in regulating autophagy/mitophagy induced by different stressors is unclear. Here, we show that c…
View article: Functional analysis of CYP4B1 enzymes from apes and humans uncovers evolutionary hot spots for adaptations of the catalytical function
Functional analysis of CYP4B1 enzymes from apes and humans uncovers evolutionary hot spots for adaptations of the catalytical function Open
A hallmark of the highly conserved CYP4B1 enzyme in mammals is the capability to bioactivate both xenobiotic and endobiotic substrates. However, due to a single amino acid change (p.P427S) within the evolutionary conserved meander region n…
View article: Supplementary Figure S2 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S2 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Optimization of the timepoint after exposure to IR for collection of cells for pKAP1 flow cytometric assays.
View article: Supplementary Figure S7 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S7 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Schematic diagram of key steps and a timeline for functional testing of ATM variants from the final cloning step to generate the lentiviral-ATMco expression construct to phospho-flow functional assays and quality assurance measures.
View article: Supplementary Figure S3 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S3 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Immunoblots demonstrating expression of WT ATM, and benign and stable pathogenic missense controls, in ATM-deficient cells, and their activities against multiple substrates.
View article: Supplementary Table S1 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Table S1 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Parameters of variants and assignment of ACMG points for controls and VUS that were functionally tested in our study.
View article: Supplementary Figure S11 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S11 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Insights into structural effects of selected deleterious variants in the N- and C-terminal lobes within the Kinase domain of human ATM.
View article: Supplementary Figure S12 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S12 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Immortalization with SV40 lg T-antigen does not affect pKAP1 phospho-flow assays in A-T and non A-T cell lines.
View article: Supplementary Table S3 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Table S3 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Representativeness of Study Participants.
View article: Data from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Data from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Purpose:ATM is a moderate-risk cancer susceptibility gene that harbors thousands of missense variants of uncertain significance (VUS) which limit the power of clinical genetic testing for cancer risk management and personalized medicine. F…
View article: Supplementary Figure S10 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S10 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
ACMG-based reclassification of the initial VUS set and controls subdivided by how they tested functionally utilizing the pKAP1 phospho-flow assay, and as three categories of standards, respectively.
View article: Supplementary Figure S8 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S8 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Immunoblots displaying expression levels for all VUS functionally tested in this study in comparison to WT ATM.
View article: Supplementary Figure S5 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S5 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Immunoblots for nptII demonstrate instability of each of the 9 pathogenic truncating ATM controls.
View article: Supplementary Figure S6 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S6 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
IR sensitivity curves for ATM-V2716A in comparison to WT ATM and two pathogenic missense controls.
View article: Supplementary Figure S9 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S9 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Calibration of pS824 KAP1 flow cytometric functional assays in an additional ATM-deficient cell line from an A-T patient.
View article: Supplementary Figure S1 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S1 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Quantification for pCHK2 and pP53 immunoblots shown in Fig. 1D.
View article: Supplementary Figure S4 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Figure S4 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Immunoblots for nptII demonstrate expression of various pathogenic missense ATM controls with differing levels of stability.
View article: Supplementary Table S2 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Supplementary Table S2 from Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Summary of controls and variants with functional results and final ACMG-based classifications.
View article: Effects of a Low Versus Moderate–High Intense Exercise Program on Innate Immune Recovery, Fitness, and Quality of Life During Pediatric Allo‐HSCT—The ANIMAL Exploratory Randomized Controlled Trial
Effects of a Low Versus Moderate–High Intense Exercise Program on Innate Immune Recovery, Fitness, and Quality of Life During Pediatric Allo‐HSCT—The ANIMAL Exploratory Randomized Controlled Trial Open
Objectives Pediatric cancer patients undergoing allogeneic hematopoietic stem cell transplantation (allo‐HSCT) are severely immunosuppressed leading to a high risk for life‐threatening infections. This trial investigates the effects of exe…
View article: SIRT4 positively regulates autophagy <i>via</i> ULK1, but independently of HDAC6 and OPA1
SIRT4 positively regulates autophagy <i>via</i> ULK1, but independently of HDAC6 and OPA1 Open
The sirtuin SIRT4 has been implicated in the control of autophagy and mitochondrial quality control via mitophagy, but the regulatory role of SIRT4 in autophagy/mitophagy induced by different stressors is unclear. Here, we show that cells …
View article: Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework
Reclassification of ATM Missense Variants of Uncertain Significance by Integrating Results from Systematic Functional Assays into an ACMG Points–Based Framework Open
Purpose: ATM is a moderate-risk cancer susceptibility gene that harbors thousands of missense variants of uncertain significance (VUS) which limit the power of clinical genetic testing for cancer risk management and personalized medicine. …
View article: Serpins A1/A3 within tumor-derived extracellular vesicles support pro-tumoral bias of neutrophils in cancer
Serpins A1/A3 within tumor-derived extracellular vesicles support pro-tumoral bias of neutrophils in cancer Open
Neutrophils are known to play an important regulatory role during tumor progression in several types of cancer. However, the mechanisms responsible for their tumorigenic bias and extended lifespan in cancer are not clear to date. This stud…
View article: Dose‐dependent expression of <scp>GFI1</scp> alters metabolism in the haematopoietic progenitors and <scp>MLL</scp>::<scp>AF9</scp>‐induced leukaemic cells
Dose‐dependent expression of <span>GFI1</span> alters metabolism in the haematopoietic progenitors and <span>MLL</span>::<span>AF9</span>‐induced leukaemic cells Open
Summary Growth factor independence 1 (GFI1) is a transcriptional repressor protein that plays an essential role in the differentiation of myeloid and lymphoid progenitors. We and other groups have shown that GFI1 has a dose‐dependent role …
View article: Understanding WT1 Alterations and Expression Profiles in Hematological Malignancies
Understanding WT1 Alterations and Expression Profiles in Hematological Malignancies Open
WT1 is a true chameleon, both acting as an oncogene and tumor suppressor. As its exact role in leukemogenesis is still ambiguous, research with model systems representing natural conditions surrounding the genetic alterations in WT1 is nec…
View article: Extracellular vesicles from immortalized mesenchymal stromal cells protect against neonatal hypoxic-ischemic brain injury
Extracellular vesicles from immortalized mesenchymal stromal cells protect against neonatal hypoxic-ischemic brain injury Open
Background Human mesenchymal stromal cell (MSC)-derived extracellular vesicles (EV) revealed neuroprotective potentials in various brain injury models, including neonatal encephalopathy caused by hypoxia–ischemia (HI). However, for clinica…
View article: Supplemental Figure S6 from Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model
Supplemental Figure S6 from Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model Open
Single-agent or combination treatment does not affect TMD231 cell invasion.
View article: Supplemental Figure S2 from Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model
Supplemental Figure S2 from Potentiation of Carboplatin-Mediated DNA Damage by the Mdm2 Modulator Nutlin-3a in a Humanized Orthotopic Breast-to-Lung Metastatic Model Open
Total apoptosis/necrosis in normal fibroblast cells.
View article: Supplementary Figures 1 - 10 from Combining Hedgehog Signaling Inhibition with Focal Irradiation on Reduction of Pancreatic Cancer Metastasis
Supplementary Figures 1 - 10 from Combining Hedgehog Signaling Inhibition with Focal Irradiation on Reduction of Pancreatic Cancer Metastasis Open
PDF file - 372K, Supplementary Figure 1 (Fig.S1)- Detection of CD24 transcript in AsPC1 cells, Supplementary Figure 2 (Fig.S2) Detection of Hh target genes in orthotopic pancreatic cancer models, Supplementary Figure 3 (Fig.S3) Expression …
View article: Supplementary Data from Exposure of Patient-Derived Mesenchymal Stromal Cells to TGFB1 Supports Fibrosis Induction in a Pediatric Acute Megakaryoblastic Leukemia Model
Supplementary Data from Exposure of Patient-Derived Mesenchymal Stromal Cells to TGFB1 Supports Fibrosis Induction in a Pediatric Acute Megakaryoblastic Leukemia Model Open
Figure S1. Cells isolated from bone marrow aspirates of ML-DS and AMKL patients as well HDs fulfilled the minimal ISCT criteria of MSCs. Figure S2. Unsupervised hierarchical clustering of MSCs without grouping according to their entities. …