Nadja A. Henke
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View article: Decoding bioprocesses with transcriptomics: current status and future potential
Decoding bioprocesses with transcriptomics: current status and future potential Open
Transcriptomic analyses represent widely used state-of-the art methodologies in molecular biosciences going back to the early 1960s. Over the last years, transcriptomics has become increasingly important in the field of bioprocess engineer…
View article: Production of Ultraviolet A protectant C50 carotenoid decaprenoxanthin by metabolically engineered Corynebacterium glutamicum
Production of Ultraviolet A protectant C50 carotenoid decaprenoxanthin by metabolically engineered Corynebacterium glutamicum Open
Carotenoids play roles as active compounds in cosmetics, food, and feed applications, for which C40 carotenoids are most common. In recent years, Corynebacterium glutamicum, a Gram-positive bacterium renowned for large-scale amino acid pro…
View article: Enabling and improving trans-nerolidol production by Corynebacterium glutamicum: combining metabolic engineering and trace elements medium refinement
Enabling and improving trans-nerolidol production by Corynebacterium glutamicum: combining metabolic engineering and trace elements medium refinement Open
Terpenes are biomolecules of significant industrial relevance, with applications in pharmaceuticals, cosmetics, and the food industry. Their biotechnological production is emerging, with Corynebacterium glutamicum , a Gram-positive bacteri…
View article: Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum
Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum Open
View article: Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum
Supercritical carbon dioxide extraction of astaxanthin from Corynebacterium glutamicum Open
Astaxanthin, a red carotenoid with potent antioxidant properties, holds significant value in the feed, cosmetics, and nutraceutical industries. While traditionally sourced from microalgae, Corynebacterium glutamicum, a well-established ind…
View article: Response surface-based media optimization for astaxanthin production in Corynebacterium glutamicum
Response surface-based media optimization for astaxanthin production in Corynebacterium glutamicum Open
Introduction Astaxanthin is a C40 carotenoid that is used in animal feeds or cosmetics. Due to its high antioxidant property it is used for, e.g., anti-aging formulations and due to its intense red color it is used, e.g., in animal feed. W…
View article: Production of the Uv-A Protectant C50 Carotenoid Decaprenoxanthin by Metabolically Engineered Corynebacterium Glutamicum
Production of the Uv-A Protectant C50 Carotenoid Decaprenoxanthin by Metabolically Engineered Corynebacterium Glutamicum Open
View article: Enhancing astaxanthin biosynthesis and pathway expansion towards glycosylated C40 carotenoids by Corynebacterium glutamicum
Enhancing astaxanthin biosynthesis and pathway expansion towards glycosylated C40 carotenoids by Corynebacterium glutamicum Open
Astaxanthin, a versatile C40 carotenoid prized for its applications in food, cosmetics, and health, is a bright red pigment with powerful antioxidant properties. To enhance astaxanthin production in Corynebacterium glutamicum , we employed…
View article: Design-of-Experiment-Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with Corynebacterium glutamicum
Design-of-Experiment-Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with Corynebacterium glutamicum Open
Corynebacterium glutamicum is prominent in the industrial production of secreted amino acids. Notably, it naturally accumulates the carotenoid pigment decaprenoxanthin in its membranes. Metabolic engineering enabled the production of astax…
View article: Design of Experiment Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with <em>Corynebacterium glutamicum</em>
Design of Experiment Guided Establishment of a Fermentative Bioprocess for Biomass-Bound Astaxanthin with <em>Corynebacterium glutamicum</em> Open
Corynebacterium glutamicum is prominent for the industrial production of secreted amino acids. Notably, it naturally accumulates the carotenoid pigment decaprenoxanthin in its membranes. Metabolic engineering enabled production of astaxant…
View article: Extraction and Purification of Highly Active Astaxanthin from Corynebacterium glutamicum Fermentation Broth
Extraction and Purification of Highly Active Astaxanthin from Corynebacterium glutamicum Fermentation Broth Open
The marine carotenoid astaxanthin is one of the strongest natural antioxidants and therefore is used in a broad range of applications such as cosmetics or nutraceuticals. To meet the growing market demand, the natural carotenoid producer C…
View article: From Aquaculture to Aquaculture: Production of the Fish Feed Additive Astaxanthin by Corynebacterium glutamicum Using Aquaculture Sidestream
From Aquaculture to Aquaculture: Production of the Fish Feed Additive Astaxanthin by Corynebacterium glutamicum Using Aquaculture Sidestream Open
Circular economy holds great potential to minimize the use of finite resources, and reduce waste formation by the creation of closed-loop systems. This also pertains to the utilization of sidestreams in large-scale biotechnological process…
View article: Functional food additives/ingredients production by engineered Corynebacterium glutamicum
Functional food additives/ingredients production by engineered Corynebacterium glutamicum Open
Corynebacterium glutamicum is a microbial production host established in the industry 60 years ago. It is mainly used for production of feed and food amino acids. As C. glutamicum strain development has been cutting edge since its discover…
View article: CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum
CRISPRi-Library-Guided Target Identification for Engineering Carotenoid Production by Corynebacterium glutamicum Open
Corynebacterium glutamicum is a prominent production host for various value-added compounds in white biotechnology. Gene repression by dCas9/clustered regularly interspaced short palindromic repeats (CRISPR) interference (CRISPRi) allows f…
View article: Improved Plasmid-Based Inducible and Constitutive Gene Expression in Corynebacterium glutamicum
Improved Plasmid-Based Inducible and Constitutive Gene Expression in Corynebacterium glutamicum Open
Corynebacterium glutamicum has been safely used in white biotechnology for the last 60 years and the portfolio of new pathways and products is increasing rapidly. Hence, expression vectors play a central role in discovering endogenous gene…
View article: Corynebacterium glutamicum CrtR and Its Orthologs in Actinobacteria: Conserved Function and Application as Genetically Encoded Biosensor for Detection of Geranylgeranyl Pyrophosphate
Corynebacterium glutamicum CrtR and Its Orthologs in Actinobacteria: Conserved Function and Application as Genetically Encoded Biosensor for Detection of Geranylgeranyl Pyrophosphate Open
Carotenoid biosynthesis in Corynebacteriumglutamicum is controlled by the MarR-type regulator CrtR, which represses transcription of the promoter of the crt operon (PcrtE) and of its own gene (PcrtR). Geranylgeranyl pyrophosphate (GGPP), a…
View article: Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein
Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein Open
Astaxanthin is one of the strongest natural antioxidants and a red pigment occurring in nature. This C40 carotenoid is used in a broad range of applications such as a colorant in the feed industry, an antioxidant in cosmetics or as a suppl…
View article: Modeling and Simulating the Aerobic Carbon Metabolism of a Green Microalga Using Petri Nets and New Concepts of VANESA
Modeling and Simulating the Aerobic Carbon Metabolism of a Green Microalga Using Petri Nets and New Concepts of VANESA Open
In this work we present new concepts of VANESA, a tool for modeling and simulation in systems biology. We provide a convenient way to handle mathematical expressions and take physical units into account. Simulation and result management ha…
View article: Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum
Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum Open
Patchoulol is a sesquiterpene alcohol and an important natural product for the perfume industry. Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual production volume of ~6…
View article: Carotenoid Production by Corynebacterium: The Workhorse of Industrial Amino Acid Production as Host for Production of a Broad Spectrum of C40 and C50 Carotenoids
Carotenoid Production by Corynebacterium: The Workhorse of Industrial Amino Acid Production as Host for Production of a Broad Spectrum of C40 and C50 Carotenoids Open
Corynebacterium glutamicum is used as a workhorse of industrial biotechnology for more than 60 years since its discovery as a natural glutamate producer in the 1950s. Nowadays, L-glutamate and L-lysine are being produced with this GRAS org…
View article: Isoprenoid Pyrophosphate-Dependent Transcriptional Regulation of Carotenogenesis in Corynebacterium glutamicum
Isoprenoid Pyrophosphate-Dependent Transcriptional Regulation of Carotenogenesis in Corynebacterium glutamicum Open
Corynebacterium glutamicum is a natural producer of the C50 carotenoid decaprenoxanthin. The crtEcg0722crtBIYEb operon comprises most of its genes for terpenoid biosynthesis. The MarR-type regulator encoded upstream and in di…
View article: Overexpression of the primary sigma factor gene sigA improved carotenoid production by Corynebacterium glutamicum : Application to production of β-carotene and the non-native linear C50 carotenoid bisanhydrobacterioruberin
Overexpression of the primary sigma factor gene sigA improved carotenoid production by Corynebacterium glutamicum : Application to production of β-carotene and the non-native linear C50 carotenoid bisanhydrobacterioruberin Open
View article: Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum
Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum Open
Astaxanthin, a red C40 carotenoid, is one of the most abundant marine carotenoids. It is currently used as a food and feed additive in a hundred-ton scale and is furthermore an attractive component for pharmaceutical and cosmetic applicati…