Herbert Schmidt
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View article: Proposal of Furfurilactobacillus cerevisiae sp. nov. isolated from spoiled beer and Furfurilactobacillus cerealis sp. nov. isolated from sourdough
Proposal of Furfurilactobacillus cerevisiae sp. nov. isolated from spoiled beer and Furfurilactobacillus cerealis sp. nov. isolated from sourdough Open
A core genome phylogenomic analysis of two lactic acid bacteria isolates from spoiled beer, Furfurilactobacillus sp. LTH 5742 and LTH 5750, one isolate from sourdough, Furfurilactobacillus sp. C5, and the type strains of five Furfurilactob…
View article: Contamination-controlled upper gastrointestinal microbiota profiling reveals salivary-duodenal community types linked to opportunistic pathogen carriage and inflammation
Contamination-controlled upper gastrointestinal microbiota profiling reveals salivary-duodenal community types linked to opportunistic pathogen carriage and inflammation Open
The upper gastrointestinal (uGI) microbiota has been implicated in infectious, metabolic, and immunological conditions, yet remains poorly characterized due to invasive sampling and low microbial biomass. We developed and validated a conta…
View article: Selection and characterization of exopolysaccharide producing lactic acid bacteria as potential food starter cultures
Selection and characterization of exopolysaccharide producing lactic acid bacteria as potential food starter cultures Open
Lactic acid bacteria (LAB) play a vital role in the production of fermented foods, with certain strains being capable of producing exopolysaccharides (EPS) that may enhance the texture and functionality of fermented food products. Among th…
View article: Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli
Excess A-subunits of Shiga toxin 2a are produced in enterohemorrhagic Escherichia coli Open
Shiga toxins (Stx) produced by Shiga toxin-producing Escherichia coli (STEC) and enterohemorrhagic E. coli (EHEC) are ribosome-inactivating AB 5 proteins that consist of one enzymatic active A-subunit (StxA) and a pentamer of non-covalentl…
View article: Phage applications for biocontrol of enterohemorrhagic E. coli O157:H7 and other Shiga toxin-producing Escherichia coli
Phage applications for biocontrol of enterohemorrhagic E. coli O157:H7 and other Shiga toxin-producing Escherichia coli Open
Foodborne outbreaks are becoming increasingly common and linked to zoonotic diseases caused by microbial spillover from wild or farm animals. Furthermore, agricultural animals could be considered reservoirs of multidrug-resistant (MDR) mic…
View article: Selection of Bacillus spp. as fermentation cultures for production of plant-based cheese analogues
Selection of Bacillus spp. as fermentation cultures for production of plant-based cheese analogues Open
Bacillus species are beneficial fermentation microbes that exhibit useful technological traits including the expression of extracellular amylolytic and proteolytic enzymes and antimicrobial lipopeptides. In this study, strains of Bacillus …
View article: Structure formation in fruit preparations by fruit fermentates produced with exopolysaccharide-forming lactic acid bacteria
Structure formation in fruit preparations by fruit fermentates produced with exopolysaccharide-forming lactic acid bacteria Open
Fruit preparations are intermediate food products that are primarily used in the dairy industry for the production of fruit yogurt or frozen desserts. Typically, they are stabilized by added hydrocolloids like pectins. The objective of thi…
View article: Complete genome sequence of <i>Kozakia baliensis</i> LTH 7215, a newly isolated strain from grape must
Complete genome sequence of <i>Kozakia baliensis</i> LTH 7215, a newly isolated strain from grape must Open
We report the complete genome sequence of the novel Kozakia baliensis strain LTH 7215, isolated from grape must. This strain exhibits a mucoid phenotype on solid medium, associated with exopolysaccharide production. The high-quality and fu…
View article: Identification and evaluation of Escherichia coli strain ATCC 8739 as a surrogate for thermal inactivation of enterohemorrhagic Escherichia coli in fruit nectars: Impact of applied techniques on the decimal reduction time
Identification and evaluation of Escherichia coli strain ATCC 8739 as a surrogate for thermal inactivation of enterohemorrhagic Escherichia coli in fruit nectars: Impact of applied techniques on the decimal reduction time Open
The objective of this study was to identify a suitable surrogate for E. coli O157:H7 strain 19685/91 and O113:H21 strain TS18/08, by assessing their thermal resistance at temperatures of 60 °C, 65 °C, and 72 °C in strawberry nectar. The in…
View article: Suitability of Escherichia coli ATCC 11229 as Salmonella enterica surrogate for strawberry nectar pasteurization
Suitability of Escherichia coli ATCC 11229 as Salmonella enterica surrogate for strawberry nectar pasteurization Open
Heat resistance, D- and z-values of five Salmonella enterica strains, namely S. Senftenberg LTH 5703, S. Typhimurium ATCC 13311 and ATCC 14028, S. Saintpaul LTH 6494, and S. Enteritidis ATCC 13076, as well as of a Salmonella cocktail and t…
View article: Rapid Acidification and Off-Flavor Reduction of Pea Protein by Fermentation with Lactic Acid Bacteria and Yeasts
Rapid Acidification and Off-Flavor Reduction of Pea Protein by Fermentation with Lactic Acid Bacteria and Yeasts Open
Pea protein is widely used as an alternative protein source in plant-based products. In the current study, we fermented pea protein to reduce off-flavor compounds, such as hexanal, and to produce a suitable fermentate for further processin…
View article: Broad time‐dependent transcriptional activity of metabolic genes of <i>E. coli</i> O104:H4 strain C227/11Φcu in a soil microenvironment at low temperature
Broad time‐dependent transcriptional activity of metabolic genes of <i>E. coli</i> O104:H4 strain C227/11Φcu in a soil microenvironment at low temperature Open
In the current study, metabolic genes and networks that influence the persistence of pathogenic Escherichia coli O104:H4 strain C227/11Φcu in agricultural soil microenvironments at low temperature were investigated. The strain was incubate…
View article: Sporadic Detection of Escherichia coli O104:H4 Strain C227/11Φcu in the Edible Parts of Lamb’s Lettuce Cultured in Contaminated Agricultural Soil Samples
Sporadic Detection of Escherichia coli O104:H4 Strain C227/11Φcu in the Edible Parts of Lamb’s Lettuce Cultured in Contaminated Agricultural Soil Samples Open
In the current study, we demonstrate that E. coli O104:H4 strain C227/11Φcu, a derivative of the 2011 enterohemorrhagic/enteroaggregative (EHEC/EAEC) E. coli outbreak strain, migrated into the edible portion of lamb’s lettuce plants upon c…
View article: The Prophage and Us—Shiga Toxin Phages Revisited
The Prophage and Us—Shiga Toxin Phages Revisited Open
The authors first met in 1998 at the University of Würzburg, Germany, at the Institute of Hygiene and Microbiology, in Helge Karch’s lab, where Herbert Schmidt worked as a PostDoc and Maite Muniesa visited the lab for a postdoctoral resear…
View article: Additional file 2 of Genome-wide association reveals host-specific genomic traits in Escherichia coli
Additional file 2 of Genome-wide association reveals host-specific genomic traits in Escherichia coli Open
Additional file 2: Table S1. Metadata of 1,198 E. coli isolates. Table S2. Genes and genetic variants associated with different hosts that came as significant by both k-mer based as well as pan-genome based GWAS. Table S3. Prevalence of om…
View article: A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli
A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli Open
Enterohemorrhagic Escherichia coli (EHEC) can cause severe diarrheic in humans. To improve therapy options, a better understanding of EHEC pathogenicity is essential. The genetic manipulation of EHEC with classical one-step methods, such a…
View article: Novel Aspects of the SubA Subunit of the Subtilase Cytotoxin
Novel Aspects of the SubA Subunit of the Subtilase Cytotoxin Open
The subtilase cytotoxin (SubAB) belongs to the family of AB5 toxins and is produced together with Shiga toxin (Stx) by certain Stx-producing E. coli strains (STEC). For most AB-type toxins, it is assumed that cytotoxic effects can only be …
View article: Genome-wide association reveals host-specific genomic traits in <i>Escherichia coli</i>
Genome-wide association reveals host-specific genomic traits in <i>Escherichia coli</i> Open
Escherichia coli is an opportunistic pathogen that can colonize or infect various host species. There is a significant gap in our understanding to what extent genetic lineages of E. coli are adapted or restricted to specific hosts. In addi…
View article: Survival of Enterohemorrhagic Escherichia coli O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on rpoS and Environmental Factors
Survival of Enterohemorrhagic Escherichia coli O104:H4 Strain C227/11Φcu in Agricultural Soils Depends on rpoS and Environmental Factors Open
The consumption of contaminated fresh produce caused outbreaks of enterohemorrhagic (EHEC) Escherichia coli. Agricultural soil might be a reservoir for EHEC strains and represent a contamination source for edible plants. Furthermore, the a…
View article: Effects of Protein, Calcium, and pH on Gene Transcription, Cell-Envelope Peptidase Activity of Lactococcus lactis Strains, and the Formation of Bitter Peptides
Effects of Protein, Calcium, and pH on Gene Transcription, Cell-Envelope Peptidase Activity of Lactococcus lactis Strains, and the Formation of Bitter Peptides Open
Calcium- and protein-rich fermented milk products, such as concentrated yoghurts and fresh cheeses, may contain undesired bitter peptides, which are generated by the proteolytic cleavage of casein. Up to now, it is not clear whether this p…
View article: Cytotoxic Effects of Recombinant StxA2-His in the Absence of Its Corresponding B-Subunit
Cytotoxic Effects of Recombinant StxA2-His in the Absence of Its Corresponding B-Subunit Open
AB5 protein toxins are produced by certain bacterial pathogens and are composed of an enzymatically active A-subunit and a B-subunit pentamer, the latter being responsible for cell receptor recognition, cellular uptake, and transport of th…
View article: Bacteriophages of Shiga Toxin-Producing Escherichia coli and Their Contribution to Pathogenicity
Bacteriophages of Shiga Toxin-Producing Escherichia coli and Their Contribution to Pathogenicity Open
Shiga toxins (Stx) of Shiga toxin-producing Escherichia coli (STEC) are generally encoded in the genome of lambdoid bacteriophages, which spend the most time of their life cycle integrated as prophages in specific sites of the bacterial ch…
View article: Influence of Iron Losses on Switching Dynamics of an Electromagnet from\n Experiment and Simulation
Influence of Iron Losses on Switching Dynamics of an Electromagnet from\n Experiment and Simulation Open
The switching behaviour of electromagnets is determined by the inertia of the\narmature, the stiffness of the return spring and the magnetostatic forces\nbetween armature and yoke. For highly dynamic systems, hysteresis and eddy\ncurrent l…