Hermann E. Gaub
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View article: Cytotoxicity of Colloidal CdSe and CdSe/ZnS Nanoparticles
Cytotoxicity of Colloidal CdSe and CdSe/ZnS Nanoparticles Open
Cytotoxicity of CdSe and CdSe/ZnS nanoparticles has been investigated for different surface modifications such as coating with mercaptopropionic\nacid, silanization, and polymer coating. For all cases, quantitative values for the onset of …
View article: Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2:ACE2 Interface and Virus Transmissibility
Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2:ACE2 Interface and Virus Transmissibility Open
Viruses mutate under a variety of selection pressures, allowing them to continuously adapt to their hosts. Mutations in SARS-CoV-2 have shown effective evasion of population immunity and increased affinity to host factors, in particular to…
View article: Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2:ACE2 Interface and Virus Transmissibility
Effects of Variants of Concern Mutations on the Force-Stability of the SARS-CoV-2:ACE2 Interface and Virus Transmissibility Open
Viruses mutate under a variety of selection pressures, allowing them to continuously adapt to their hosts. Mutations in SARS-CoV-2 have shown effective evasion of population immunity and increased affinity to host factors, in particular to…
View article: A tethered ligand assay to probe SARS-CoV-2:ACE2 interactions
A tethered ligand assay to probe SARS-CoV-2:ACE2 interactions Open
Significance In the dynamic environment of the airways, where SARS-CoV-2 infections are initiated by binding to human host receptor ACE2, mechanical stability of the viral attachment is a crucial fitness advantage. Using single-molecule fo…
View article: A Tethered Ligand Assay to Probe SARS-CoV-2:ACE2 Interactions
A Tethered Ligand Assay to Probe SARS-CoV-2:ACE2 Interactions Open
SARS-CoV-2 infections are initiated by attachment of the receptor-binding domain (RBD) on the viral Spike protein to angiotensin-converting enzyme-2 (ACE2) on human host cells. This critical first step occurs in dynamic environments, where…
View article: A Tethered Ligand Assay to Probe SARS-CoV-2:ACE2 Interactions
A Tethered Ligand Assay to Probe SARS-CoV-2:ACE2 Interactions Open
SARS-CoV-2 infections are initiated by attachment of the receptor-binding domain (RBD) on the viral Spike protein to angiotensin-converting enzyme-2 (ACE2) on human host cells. This critical first step occurs in dynamic environments, where…
View article: Kinetic Interval Measurement: A Tool to Characterize Thermal Reversion Dynamics of Light-switchable Fluorescent Proteins
Kinetic Interval Measurement: A Tool to Characterize Thermal Reversion Dynamics of Light-switchable Fluorescent Proteins Open
Light-switchable proteins like Light-Oxygen-Voltage (LOV) domains can be used to control protein interactions and have been applied in vivo to manipulate cell behavior. The switching between dark and light state of LOV domains depends on t…
View article: Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems
Atomic Force Microscopy-Based Force Spectroscopy and Multiparametric Imaging of Biomolecular and Cellular Systems Open
During the last three decades, a series of key technological improvements turned atomic force microscopy (AFM) into a nanoscopic laboratory to directly observe and chemically characterize molecular and cell biological systems under physiol…
View article: A Tethered Ligand Assay to Probe the SARS-CoV-2 ACE2 Interaction under Constant Force
A Tethered Ligand Assay to Probe the SARS-CoV-2 ACE2 Interaction under Constant Force Open
The current COVID-19 pandemic has a devastating global impact and is caused by the SARS-CoV-2 virus. SARS-CoV-2 attaches to human host cells through interaction of its receptor binding domain (RBD) located on the viral Spike (S) glycoprote…
View article: Designed Anchoring Geometries Determine Lifetimes of Biotin-Streptavidin Bonds under Constant Load and Enable Ultra-Stable Coupling
Designed Anchoring Geometries Determine Lifetimes of Biotin-Streptavidin Bonds under Constant Load and Enable Ultra-Stable Coupling Open
The small molecule biotin and the homotetrameric protein streptavidin (SA) form a stable and robust complex that plays a pivotal role in many biotechnological and medical applications. In particular, the biotin-streptavidin linkage is freq…
View article: Streptavidin/biotin: Tethering geometry defines unbinding mechanics
Streptavidin/biotin: Tethering geometry defines unbinding mechanics Open
Single-molecule force spectroscopy reveals how the mechanical stability of a widely used biomolecular complex varies fourfold.
View article: Single‐Molecule Manipulation in Zero‐Mode Waveguides
Single‐Molecule Manipulation in Zero‐Mode Waveguides Open
The mechanobiology of receptor–ligand interactions and force‐induced enzymatic turnover can be revealed by simultaneous measurements of force response and fluorescence. Investigations at physiologically relevant high labeled substrate conc…
View article: Mechanisms of Nanonewton Mechanostability in a Protein Complex Revealed by Molecular Dynamics Simulations and Single-Molecule Force Spectroscopy
Mechanisms of Nanonewton Mechanostability in a Protein Complex Revealed by Molecular Dynamics Simulations and Single-Molecule Force Spectroscopy Open
Can molecular dynamics simulations predict the mechanical behavior of protein complexes? Can simulations decipher the role of protein domains of unknown function in large macromolecular complexes? Here, we employ a wide-sampling computatio…
View article: Is mechanical receptor ligand dissociation driven by unfolding or unbinding?
Is mechanical receptor ligand dissociation driven by unfolding or unbinding? Open
Mechanical force can play a pivotal role in biological systems. Single Molecule Force Spectroscopy, is a powerful tool to probe the mechanics of proteins and their binding partners. Yet, it remains unclear how complex dissociation of a pro…
View article: Structural and mechanistic insights into mechanoactivation of focal adhesion kinase
Structural and mechanistic insights into mechanoactivation of focal adhesion kinase Open
Focal adhesion kinase (FAK) is a key signaling molecule regulating cell adhesion, migration, and survival. FAK localizes into focal adhesion complexes formed at the cytoplasmic side of cell attachment to the ECM and is activated after forc…
View article: Atomic force microscopy-based mechanobiology
Atomic force microscopy-based mechanobiology Open
Mechanobiology emerges at the crossroads of medicine, biology, biophysics and engineering and describes how the responses of proteins, cells, tissues and organs to mechanical cues contribute to development, differentiation, physiology and …
View article: Calcium stabilizes the strongest protein fold
Calcium stabilizes the strongest protein fold Open
Staphylococcal pathogens adhere to their human targets with exceptional resilience to mechanical stress, some propagating force to the bacterium via small, Ig-like folds called B domains. We examine the mechanical stability of these folds …
View article: Atomic force microscopy-based mechanobiology
Atomic force microscopy-based mechanobiology Open
Mechanobiology emerges at the crossroads of medicine, biology, biophysics and engineering and describes how the responses of proteins, cells, tissues and organs to mechanical cues contribute to development, differentiation, physiology and …
View article: Direction Matters: Monovalent Streptavidin/Biotin Complex under Load
Direction Matters: Monovalent Streptavidin/Biotin Complex under Load Open
Novel site-specific attachment strategies combined with improvements of computational resources enable new insights into the mechanics of the monovalent biotin/streptavidin complex under load and forced us to rethink the diversity of ruptu…
View article: Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy Open
In recent years, atomic force microscopy (AFM) based single molecule force spectroscopy (SMFS) extended our understanding of molecular properties and functions. It gave us the opportunity to explore a multiplicity of biophysical mechanisms…
View article: Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy Open
In recent years, atomic force microscopy (AFM) based single molecule force spectroscopy (SMFS) extended our understanding of molecular properties and functions. It gave us the opportunity to explore a multiplicity of biophysical mechanisms…
View article: Molecular mechanism of extreme mechanostability in a pathogen adhesin
Molecular mechanism of extreme mechanostability in a pathogen adhesin Open
How a pathogen holds on to its host Staphylococcus epidermidis and Staphylococcus aureus are pathogens that can form biofilms on implants and medical devices. Central to biofilm formation is a very tight interaction between microbial surfa…
View article: Monomeric streptavidin: a versatile regenerative handle for force spectroscopy
Monomeric streptavidin: a versatile regenerative handle for force spectroscopy Open
Most avidin-based handles in force spectroscopy are tetravalent biotin binders. Tetravalency presents two issues: multiple pulling geometries as well as multiple targets bound simultaneously. Additionally, such tetravalent handles require …
View article: Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry
Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry Open
The widely used interaction of the homotetramer streptavidin with the small molecule biotin has been intensively studied by force spectroscopy and has become a model system for receptor ligand interaction. However, streptavidin's tetravale…