Hermann J. Gruber
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View article: Mononuclear cell-intrinsic microRNA-21 is linked to circulating progenitor cell mobilisation during acute exercise in HFpEF
Mononuclear cell-intrinsic microRNA-21 is linked to circulating progenitor cell mobilisation during acute exercise in HFpEF Open
Background Acute exhaustive exercise acts as a physiological stressor, leading to endothelial activation and the release of circulating progenitor cells (CPC). In heart failure, this CPC response may be blunted. Reactive oxygen species pro…
View article: Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy
Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy Open
In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining…
View article: AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density
AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density Open
Ligand binding to receptors is one of the most important regulatory elements in biology as it is the initiating step in signaling pathways and cascades. Thus, precisely localizing binding sites and measuring interaction forces between cogn…
View article: Detailed Evidence for an Unparalleled Interaction Mode between Calmodulin and Orai Proteins
Detailed Evidence for an Unparalleled Interaction Mode between Calmodulin and Orai Proteins Open
Calmodulin (CaM) binds most of its targets by wrapping around an amphipathic α‐helix. The N‐terminus of Orai proteins contains a conserved CaM‐binding segment but the binding mechanism has been only partially characterized. Here, microscal…
View article: Stable Europium(III) Complexes with Short Linkers for Site‐Specific Labeling of Biomolecules
Stable Europium(III) Complexes with Short Linkers for Site‐Specific Labeling of Biomolecules Open
In this study, two new terpyridine‐based Eu III complexes were synthesized, the structures of which were optimized for luminescence resonance energy‐transfer (LRET) experiments. The complexes showed high quantum yields (32 %); a single lon…
View article: Regenerative biosensor for use with biotinylated bait molecules
Regenerative biosensor for use with biotinylated bait molecules Open
Label-free biosensors are ideally suited for the quantitative analysis of specific interactions among biomolecules or of biomolecules with drugs, as well as for quantitation of diagnostic markers in biofluids. In contrast to the label-depe…
View article: Combined Recognition Imaging and Force Spectroscopy: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density
Combined Recognition Imaging and Force Spectroscopy: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density Open
We combined recognition imaging and force spectroscopy to study the interactions between receptors and ligands on the single molecule level. This method allowed the selection of a single receptor molecule reconstituted in a supported lipid…
View article: Mutual A domain interactions in the force sensing protein von Willebrand factor
Mutual A domain interactions in the force sensing protein von Willebrand factor Open
The von Willebrand factor (VWF) is a glycoprotein in the blood that plays a central role in hemostasis. Among other functions, VWF is responsible for platelet adhesion at sites of injury via its A1 domain. Its adjacent VWF domain A2 expose…
View article: Regenerative biosensor chips based on switchable mutants of avidin—A systematic study
Regenerative biosensor chips based on switchable mutants of avidin—A systematic study Open
Biotinylated bait molecules can be immobilized on biotinylated sensor chips by formation of biotin–avidin–biotin bridges which are very stable when using wild-type (strept)avidin. Stable immobilization of biotinylated baits is important fo…
View article: The role of transporter ectodomains in drug recognition and binding: phlorizin and the sodium–glucose cotransporter
The role of transporter ectodomains in drug recognition and binding: phlorizin and the sodium–glucose cotransporter Open
This article reviews the role of segments of SLCs located outside the plasma membrane bilayer (ectodomains) using the inhibition of SGLTs (SLC5 family) by the aromatic glucoside phlorizin as a model system.