Hirotaka Ata
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View article: Rapid, Amplification-free, RNA-based Uropathogen Detection With LbuCas13a
Rapid, Amplification-free, RNA-based Uropathogen Detection With LbuCas13a Open
Background Expeditious identification of bacterial infection remains an important challenge in an emergency department. Bacterial cultures remain the gold standard, though they take 24–72 hours to result. Polymerase chain reaction-based di…
View article: Precision acute medical care through “-omic” analyses: a scoping review
Precision acute medical care through “-omic” analyses: a scoping review Open
While -omics research in acute care is growing, it remains predominantly observational with limited clinical implementation. Barriers include delayed turnaround times, insufficient EHR integration, and underrepresentation of vulnerable pop…
View article: A Primer Genetic Toolkit for Exploring Mitochondrial Biology and Disease Using Zebrafish
A Primer Genetic Toolkit for Exploring Mitochondrial Biology and Disease Using Zebrafish Open
Mitochondria are a dynamic eukaryotic innovation that play diverse roles in biology and disease. The mitochondrial genome is remarkably conserved in all vertebrates, encoding the same 37-gene set and overall genomic structure, ranging from…
View article: Chimeric RNA: DNA TracrRNA Improves Homology-Directed Repair <i>In Vitro</i> and <i>In Vivo</i>
Chimeric RNA: DNA TracrRNA Improves Homology-Directed Repair <i>In Vitro</i> and <i>In Vivo</i> Open
Nearly 90% of human pathogenic mutations are caused by small genetic variations, and methods to correct these errors efficiently are critically important. One way to make small DNA changes is providing a single-stranded oligo deoxynucleoti…
View article: Chimeric RNA:DNA Donorguide Improves HDR <i>in vitro</i> and <i>in vivo</i>
Chimeric RNA:DNA Donorguide Improves HDR <i>in vitro</i> and <i>in vivo</i> Open
Introducing small genetic changes to study specific mutations or reverting clinical mutations to wild type has been an area of interest in precision genomics for several years. In fact, it has been found that nearly 90% of all human pathog…
View article: The Gene Sculpt Suite: a set of tools for genome editing
The Gene Sculpt Suite: a set of tools for genome editing Open
The discovery and development of DNA-editing nucleases (Zinc Finger Nucleases, TALENs, CRISPR/Cas systems) has given scientists the ability to precisely engineer or edit genomes as never before. Several different platforms, protocols and v…
View article: A primer genetic toolkit for exploring mitochondrial biology and disease using zebrafish
A primer genetic toolkit for exploring mitochondrial biology and disease using zebrafish Open
Mitochondria are a dynamic eukaryotic innovation that play diverse roles in biology and disease. The mitochondrial genome is remarkably conserved in all vertebrates, encoding the same 37 gene set and overall genomic structure ranging from …
View article: Robust activation of microhomology-mediated end joining for precision gene editing applications
Robust activation of microhomology-mediated end joining for precision gene editing applications Open
One key problem in precision genome editing is the unpredictable plurality of sequence outcomes at the site of targeted DNA double stranded breaks (DSBs). This is due to the typical activation of the versatile Non-homologous End Joining (N…
View article: Toward Precision Molecular Surgery: Robust, Selective Induction of Microhomology-mediated End Joining <i>in vivo</i>
Toward Precision Molecular Surgery: Robust, Selective Induction of Microhomology-mediated End Joining <i>in vivo</i> Open
One key problem in precision genome editing is the resultant unpredictable plurality of sequence outcomes at the site of targeted DNA double-strand breaks (DSBs). This is due to the typical activation of the versatile Non-homologous End Jo…
View article: Engineering targeted deletions in the mitochondrial genome
Engineering targeted deletions in the mitochondrial genome Open
Summary Paragraph Mitochondria are a network of critical intracellular organelles with diverse functions ranging from energy production to cell signaling. The mitochondrial genome (mtDNA) consists of 37 genes that support oxidative phospho…
View article: Failure to detect DNA-guided genome editing using Natronobacterium gregoryi Argonaute
Failure to detect DNA-guided genome editing using Natronobacterium gregoryi Argonaute Open
View article: ssDNA and the Argonautes: The Quest for the Next Golden Editor
ssDNA and the Argonautes: The Quest for the Next Golden Editor Open
Genome engineering has gone mainstream because of breakthroughs in defining and harnessing naturally occurring, customizable DNA recognition cursors (protein or RNA-guided). At present, most gene editing relies on these cursors to direct c…
View article: Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping
Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping Open
Customizable endonucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) enable rapid generation of mutant strains a…