Hiroya Kuwahara
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View article: Refining Muscle Morphometry Through Machine Learning and Spatial Analysis
Refining Muscle Morphometry Through Machine Learning and Spatial Analysis Open
Aims Muscle morphology provides important information in differentiating disease aetiology, but its measurement remains challenging because of the lack of an efficient and objective method. This study aimed to quantitatively refine the mor…
View article: 18F-FAZA, 18F-FDG, and 123I-IMP Imaging Reveal Hypoxia and Metabolism in Acute MOGAD
18F-FAZA, 18F-FDG, and 123I-IMP Imaging Reveal Hypoxia and Metabolism in Acute MOGAD Open
We report the first case of myelin oligodendrocyte glycoprotein antibody–associated disease (MOGAD) evaluated with hypoxic imaging using 18 F-FAZA PET/CT. A healthy woman in her 20s presented to our hospital with seizures, headaches, and v…
View article: Anti-NF155 antibody positive autoimmune nodopathy presenting as multiple mononeuropathy with predominantly superficial sensory disturbances
Anti-NF155 antibody positive autoimmune nodopathy presenting as multiple mononeuropathy with predominantly superficial sensory disturbances Open
Anti-NF155 antibody positive autoimmune nodopathy typically presents with symmetric deep sensory disturbance, muscle weakness and tremor. Here, we report a unique case of anti-NF155 antibody positive autoimmune nodopathy presenting as mult…
View article: DNA/RNA heteroduplex technology with cationic oligopeptide reduces class-related adverse effects of nucleic acid drugs
DNA/RNA heteroduplex technology with cationic oligopeptide reduces class-related adverse effects of nucleic acid drugs Open
Antisense oligonucleotides (ASOs) are a therapeutic modality for incurable diseases. However, systemic injection of gapmer-type ASOs causes class-related toxicities, including prolongation of activated partial thromboplastin time (aPTT) an…
View article: Favorable efficacy and reduced acute neurotoxicity by antisense oligonucleotides with 2′,4′ -BNA/LNA with 9-(aminoethoxy)phenoxazine
Favorable efficacy and reduced acute neurotoxicity by antisense oligonucleotides with 2′,4′ -BNA/LNA with 9-(aminoethoxy)phenoxazine Open
[This corrects the article DOI: 10.1016/j.omtn.2024.102161.].
View article: Comparison of interaction between antimiR and microRNA versus HDO-antimiR and microRNA by molecular dynamics simulation
Comparison of interaction between antimiR and microRNA versus HDO-antimiR and microRNA by molecular dynamics simulation Open
Recently, we found DNA/RNA heteroduplex oligonucleotide-based antimiR (HDO-antimiR) can more efficiently inhibit the target miRNA than conventional antimiR after its cellular uptake. But the mechanism of HDO-antimiR about the target-silenc…
View article: Properties of artificial cationic oligodiaminosaccharides and oligopeptides that bind to A-type oligonucleotide duplexes
Properties of artificial cationic oligodiaminosaccharides and oligopeptides that bind to A-type oligonucleotide duplexes Open
A critical strategy to improve the properties of oligonucleotide therapeutics is using cationic molecules as carriers. We developed artificial cationic molecules that bind to A-type oligonucleotide duplexes, such as siRNAs, in a stoichiome…
View article: Spinocerebellar Ataxia Type 31 Exacerbated by Anti-amino Terminal of Alpha-enolase Autoantibodies
Spinocerebellar Ataxia Type 31 Exacerbated by Anti-amino Terminal of Alpha-enolase Autoantibodies Open
We herein report a 61-year-old woman who was genetically diagnosed with spinocerebellar ataxia type 31 whose symptoms were modified by anti-amino terminal of alpha-enolase (NAE) antibodies, known as a biomarker of Hashimoto's encephalopath…
View article: Assessment of Mechanisms of HDO-AntimiR by Molecular Dynamics Simulation
Assessment of Mechanisms of HDO-AntimiR by Molecular Dynamics Simulation Open
Recently, we found DNA/RNA heteroduplex oligonucleotide-based antimiR (HDO-antimiR) can more efficiently inhibit the target miRNA than conventional antimiR after its cellular uptake. But the mechanism of HDO-antimiR about the target-silenc…
View article: Solid-Phase Stereocontrolled Synthesis of Oligomeric P-Modified Glycosyl Phosphate Derivatives Using the Oxazaphospholidine Method
Solid-Phase Stereocontrolled Synthesis of Oligomeric P-Modified Glycosyl Phosphate Derivatives Using the Oxazaphospholidine Method Open
Glycosyl phosphate repeating units can be found in the glycoconjugates of some bacteria and protozoa parasites. These structures and their P-modified analogs are attractive synthetic targets as antimicrobial, antiparasitic, and vaccine age…
View article: Synthesis of Glycosyl Phosphate Repeats and Their Analogues
Synthesis of Glycosyl Phosphate Repeats and Their Analogues Open
Glycosyl phosphate repeat structures are seen in bacterial- and protozoan-derived sugar chains and these sugar chains have ten or more glycosyl phosphate repeats in many cases. Chemical synthesis of glycosyl phosphate repeats and their ana…
View article: Solution-phase synthesis of oligodeoxyribonucleotides using the <i>H</i>-phosphonate method with <i>N</i>-unprotected 5′-phosphite monomers
Solution-phase synthesis of oligodeoxyribonucleotides using the <i>H</i>-phosphonate method with <i>N</i>-unprotected 5′-phosphite monomers Open
A new strategy for a solution-phase synthesis of oligodeoxyribonucleotides with 5′-phosphite monomers synthesized in a single step from unprotected 2′-deoxyribonucleosides.
View article: Enhanced Immunostimulatory Activity of CpG Oligodeoxynucleotide by the Combination of Mannose Modification and Incorporation into Nanostructured DNA
Enhanced Immunostimulatory Activity of CpG Oligodeoxynucleotide by the Combination of Mannose Modification and Incorporation into Nanostructured DNA Open
The immunostimulatory activity of unmethylated cytosine-phosphate-guanine oligodeoxynucleotide (CpG ODN) could be improved via delivery to immune cells expressing Toll-like receptor 9 (TLR9). Previously, we showed that the polypod-like str…
View article: Highly efficient gene silencing in mouse brain by overhanging‐duplex oligonucleotides <i>via</i> intraventricular route
Highly efficient gene silencing in mouse brain by overhanging‐duplex oligonucleotides <i>via</i> intraventricular route Open
Gapmer‐type antisense oligonucleotides have not yet been approved for the treatment of central nervous system diseases, whereas steric‐blocking‐type antisense oligonucleotides have been well‐developed for clinical use. We here characterize…
View article: An efficient drug delivery system crossing the blood-brain barrier in response to glycemic control
An efficient drug delivery system crossing the blood-brain barrier in response to glycemic control Open
筆者らは、脳の主要なエネルギー源であるグルコースの、脳微小血管内皮細胞におけるグルコーストランスポーター1（GLUT1）を介した生理学的な輸送経路を活用して、血液脳関門を効率的に通過するDDSを開発している。過去の研究から、薬物キャリアの表層にグルコースをリガンドとして修飾するのみでは、静脈内投与で脳への十分な送達は実現できないと考えられたことから、筆者らは血中グルコース濃度の制御という独自の生物学的手法を導入した。薬物キャリアとしての特性をもつ高分子ミセル型ナノ粒子の表層…
View article: Overview of Clinical Trials Act
Overview of Clinical Trials Act Open
臨床研究で生じたデータの操作や利益相反行為という不正事案を受け，臨床研究法が2018年4月1日に施行された．臨床研究を推進することと規制することのバランスを考慮し，臨床研究法は，臨床研究の実施に係る行動主体（当局，臨床研究を実施する者，認定臨床研究審査委員会，医薬品等製造販売業者など）及び行動主体間の関係について最低限のルールを定め，臨床研究の手続きの明確化と質の充実を図ったものである．今後も引き続き，信頼性の高い臨床研究が実施される環境整備を進めていきたい．
View article: Intrathecal IgG Synthesis and Persistent Inflammation Are Associated with White Matter Lesions in HIV-negative Patients with Cryptococcal Meningoencephalitis
Intrathecal IgG Synthesis and Persistent Inflammation Are Associated with White Matter Lesions in HIV-negative Patients with Cryptococcal Meningoencephalitis Open
Objective Cryptococcal meningoencephalitis (CM) causes significant morbidity and mortality in human immunodeficiency virus (HIV)-negative and HIV-positive populations. White matter lesions (WMLs) have been reported in both populations of C…
View article: Artificial cationic peptides that increase nuclease resistance of siRNA without disturbing RNAi activity
Artificial cationic peptides that increase nuclease resistance of siRNA without disturbing RNAi activity Open
Properties of cationic peptides bearing amino or guanidino groups with various side chain lengths that bind to double stranded RNAs (dsRNAs) were investigated. Peptides with shorter side chain lengths effectively bound to dsRNAs (12mers) i…
View article: P1‐103: DOT BLOT ASSAY FOR QUANTITATIVE MEASUREMENT OF AMYLOID BETA OLIGOMER
P1‐103: DOT BLOT ASSAY FOR QUANTITATIVE MEASUREMENT OF AMYLOID BETA OLIGOMER Open
Soluble amyloid beta oligomers (AβOs) are believed to cause synaptic failure in Alzheimer's disease. Because the AβO has various forms and might contain some unstable forms, it is difficult to quantify AβO in degenerative status, such as S…
View article: Angubindin-1 opens the blood–brain barrier in vivo for delivery of antisense oligonucleotide to the central nervous system
Angubindin-1 opens the blood–brain barrier in vivo for delivery of antisense oligonucleotide to the central nervous system Open
Within the field of RNA therapeutics, antisense oligonucleotide-based therapeutics are a potentially powerful means of treating intractable diseases. However, if these therapeutics are used for the treatment of neurological disorders, safe…
View article: Cover Feature: Solid‐Phase Synthesis of Fluorinated Analogues of Glycosyl 1‐Phosphate Repeating Structures from <i>Leishmania</i> using the Phosphoramidite Method (ChemistryOpen 6/2018)
Cover Feature: Solid‐Phase Synthesis of Fluorinated Analogues of Glycosyl 1‐Phosphate Repeating Structures from <i>Leishmania</i> using the Phosphoramidite Method (ChemistryOpen 6/2018) Open
\nThe Cover Feature shows the structure of the longest analogue of chemically synthesized glycosyl 1‐phosphate repeating structure from Leishmania. More information can be found in the Full Paper by R. Iwata Hara et al. on page 439 in Issu…
View article: Solid‐Phase Synthesis of Fluorinated Analogues of Glycosyl 1‐Phosphate Repeating Structures from <i>Leishmania</i> using the Phosphoramidite Method
Solid‐Phase Synthesis of Fluorinated Analogues of Glycosyl 1‐Phosphate Repeating Structures from <i>Leishmania</i> using the Phosphoramidite Method Open
Bacterial and protozoan sugar chains contain glycosyl 1‐phosphate repeating structures; these repeating structures have been studied for vaccine development. The fluorinated analogues of [β‐Gal‐(1→4)‐α‐Man‐(1→6)‐P‐] n , which are glycosyl …
View article: Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo
Modulation of blood-brain barrier function by a heteroduplex oligonucleotide in vivo Open
The blood-brain barrier (BBB) is increasingly regarded as a dynamic interface that adapts to the needs of the brain, responds to physiological changes, and gets affected by and can even promote diseases. Modulation of BBB function at the m…
View article: Artificial cationic oligosaccharides for heteroduplex oligonucleotide-type drugs
Artificial cationic oligosaccharides for heteroduplex oligonucleotide-type drugs Open
View article: Correction: Enhancement in RNase H activity of a DNA/RNA hybrid duplex using artificial cationic oligopeptides
Correction: Enhancement in RNase H activity of a DNA/RNA hybrid duplex using artificial cationic oligopeptides Open
Correction for ‘Enhancement in RNase H activity of a DNA/RNA hybrid duplex using artificial cationic oligopeptides’ by Rintaro Iwata Hara et al., Chem. Commun., 2018, 54, 8526–8529.
View article: Glycaemic control boosts glucosylated nanocarrier crossing the BBB into the brain
Glycaemic control boosts glucosylated nanocarrier crossing the BBB into the brain Open
View article: Synthesis of 2′-O-monohaloethoxymethyl-modified RNAs and their duplex formation ability
Synthesis of 2′-O-monohaloethoxymethyl-modified RNAs and their duplex formation ability Open
We synthesized 2′-O-monohaloethoxymethyl-modified RNAs and evaluated their duplex formation ability.
View article: Enteral siRNA delivery technique for therapeutic gene silencing in the liver via the lymphatic route
Enteral siRNA delivery technique for therapeutic gene silencing in the liver via the lymphatic route Open
An efficient targeting delivery technology is needed for functional oligonucleotides to exert their potential effect on the target gene without an adverse effect in vivo . Development of enteral delivery systems for nucleic acids is a majo…
View article: DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing
DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing Open
View article: Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol
Chimeric Antisense Oligonucleotide Conjugated to α-Tocopherol Open