Hsing‐Jien Kung
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View article: Identifying Rare Germline Variants Associated with Metastatic Prostate Cancer Through an Extreme Phenotype Study
Identifying Rare Germline Variants Associated with Metastatic Prostate Cancer Through an Extreme Phenotype Study Open
Background Studies of germline variants in prostate cancer (PCa) have largely focused on their connections to cancer predisposition. However, an understanding of how heritable factors contribute to cancer progression and metastasis remain …
View article: Longitudinal single-cell RNA sequencing of a neuroendocrine transdifferentiation model reveals transcriptional reprogramming in treatment-induced neuroendocrine prostate cancer
Longitudinal single-cell RNA sequencing of a neuroendocrine transdifferentiation model reveals transcriptional reprogramming in treatment-induced neuroendocrine prostate cancer Open
Neuroendocrine transdifferentiation (NEtD) of prostate adenocarcinoma (PRAD) leads to aggressive neuroendocrine prostate cancer (NEPC). The LTL331 patient-derived xenograft (PDX) model consistently progresses to NEPC following castration, …
View article: KDM4C works in concert with GATA1 to regulate heme metabolism in head and neck squamous cell carcinoma
KDM4C works in concert with GATA1 to regulate heme metabolism in head and neck squamous cell carcinoma Open
Head and neck squamous cell carcinoma (HNSCC), the sixth most common cancer worldwide, presents significant public health challenges due to its genetic instability and late-stage diagnosis. Despite advancements in treatment, the median ove…
View article: Functional inversion of circadian regulator REV-ERBα leads to tumorigenic gene reprogramming
Functional inversion of circadian regulator REV-ERBα leads to tumorigenic gene reprogramming Open
Profound functional switch of key regulatory factors may play a major role in homeostasis and disease. Dysregulation of circadian rhythm (CR) is strongly implicated in cancer with mechanisms poorly understood. We report here that the funct…
View article: Figure S9 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S9 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Influence of HIF1A-AS2 on normal human gingival epithelial cells.
View article: Figure S4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
The impact of ectopic HIF1A-AS2 on the expression of HLA-ABC in different human cancer cell lines.
View article: Table S3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Primary antibodies used in this study.
View article: Table S1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Primers for cloning constructs and plasmid information.
View article: Table S4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Characteristics of the HNSCC patients for in situ hybridization and immunohistochemistry analysis (n = 29).
View article: Figure S5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
HIF1A-AS2 does not affect the expression of autophagy-related genes.
View article: Figure 6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
HIF1A-AS2 is associated with an aggressive clinical stage in HNSCC. A, The expression of HIF1A-AS2 in different clinical stages of patients with GDC-HNSCC (n = 515). The whiskers extend to the minimum and maximu…
View article: Figure 5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
HIF1A-AS2 interacts with NBR1 and MHC-I to mediate autophagic degradation of MHC-I. A, Co-immunoprecipitation (Co-IP) to detect ubiquitination of HLA-A with or without the existence of HIF1A-AS2. 293T cells were co-tra…
View article: Table S6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Reagents and resources used in this study.
View article: FIgure S7 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
FIgure S7 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Multispectral immunofluorescent staining for analyzing the infiltrated immune cells in HNSCC samples.
View article: Figure 3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Impact of HIF1A-AS2 on the expression of MHC-Ⅰ in HNSCC. A, CIBERSORT analysis to compare the proportion of infiltrated immune cells in patients from the TCGA-HNSCC database with high HIF1A-AS2 [HIF1A-AS2(H)] versus lo…
View article: Figure 7 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 7 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Schematic representation of the study.
View article: Figure 1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Hypoxia-induced HIF1A-AS2 enrichment in HNSCC cells and HNSCC-TEXs. A, Heatmaps for showing the RT-qPCR results of the hypoxia relative lncRNA expression in the cellular (left) and exosomal (right) contents of SAS cells. For …
View article: Figure S3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S3 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
HIF1A-AS2 does not influence the characteristics of HNSCC cells.
View article: Figure S2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
The experimental replicates of ChIP assays.
View article: Figure 4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 4 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
HIF1A-AS2 downregulates MHC-I through autophagic degradation. A, Western blots showing the expression of HLA-ABC, STAT1 in SAS cells overexpressing HIF1A-AS2 (SAS-HIF1A-AS2) versus SAS-control (SAS-Vec) treated with/wi…
View article: Figure S1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S1 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Purification and validation of the exosomes from hypoxic HNSCC
View article: Figure S8 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S8 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Representative images of HIF-1α, HLA-ABC, and HIF1A-AS2 in the stroma, epithelium, and tumor part of a HNSCC sample.
View article: Table S5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S5 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Characteristics of the HNSCC patients for immunohistochemistry analysis (n = 57).
View article: Data from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Data from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Intratumoral hypoxia not only promotes angiogenesis and invasiveness of cancer cells but also creates an immunosuppressive microenvironment that facilitates tumor progression. However, the mechanisms by which hypoxic tumor cells disseminat…
View article: Table S2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Table S2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Primer list for quantitative PCR & ChIP.
View article: Figure S6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure S6 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
The representative images of manual IHC scoring and quantification of tumor-infiltrated CD8+ cells in HNSCC samples.
View article: Figure 2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Figure 2 from Hypoxia-Induced Long Noncoding RNA <i>HIF1A-</i><i>AS2</i> Regulates Stability of MHC Class I Protein in Head and Neck Cancer Open
Direct regulation of HIF1A-AS2 by HIF1α. A, ChIP-seq analysis of the HIF1α binding on the regulatory region of HIF1A-AS2 in human umbilical vein cell line (EA.hy926), human prostatic adenocarcinoma cell (PC3), human re…
View article: Hydrogen sulfide coordinates glucose metabolism switch through destabilizing tetrameric pyruvate kinase M2
Hydrogen sulfide coordinates glucose metabolism switch through destabilizing tetrameric pyruvate kinase M2 Open
Most cancer cells reprogram their glucose metabolic pathway from oxidative phosphorylation to aerobic glycolysis for energy production. By reducing enzyme activity of pyruvate kinase M2 (PKM2), cancer cells attain a greater fraction of gly…
View article: Hydrogen Sulfide Coordinates Glucose Metabolism Switch through Destabilizing Tetrameric Pyruvate Kinase M2
Hydrogen Sulfide Coordinates Glucose Metabolism Switch through Destabilizing Tetrameric Pyruvate Kinase M2 Open
Cancer cells reprogram their glucose metabolic pathway from oxidative phosphorylation toward aerobic glycolysis. Pyruvate kinase M2 (PKM2), which converts phosphoenolpyruvate (PEP) to pyruvate, is considered the rate-limiting enzyme involv…
View article: Targeting androgen receptor and the variants by an orally bioavailable Proteolysis Targeting Chimeras compound in castration resistant prostate cancer
Targeting androgen receptor and the variants by an orally bioavailable Proteolysis Targeting Chimeras compound in castration resistant prostate cancer Open