Explanipedia

Theoretical Investigation of the Material Usage During On-Bead Enrichment of Post-Translationally Modified Peptides in Suspension Systems Open

Kai Liu, Yuanyu Huang, Thomas W. Huang, Pengyuan Yang, Jilie Kong , et al. · 2025

Over the past decade, the number and diversity of identified protein post-translational modifications (PTMs) have grown significantly. However, most PTMs occur at relatively low abundance, making selective enrichment of modified peptides e…

Ultradeep N-glycoproteome atlas of mouse reveals spatiotemporal signatures of brain aging and neurodegenerative diseases Open

Pan Fang, Xiangming Yu, Min Ding, Qifei Cong, Hongyu Jiang , et al. · 2025

Multi-Omics Analysis Unveils Nsun5-Mediated Molecular Alterations in the Somatosensory Cortex and Its Impact on Pain Sensation Open

Peipei Chen, Heyu Zhao, Xia Gao, Junchao Xu, Zhi‐Li Huang , et al. · 2025

Nsun5 assumes a pivotal role in the regulation of RNA methylation, and its deficiency has been linked to the advancement of hepatocellular carcinoma, gliomas, tetralogy of Fallot, cognitive deficits in Williams-Beuren syndrome, and brain d…

Spatiotemporal proteomic and transcriptomic landscape of DAT+ dopaminergic neurons development and function Open

Heyu Zhao, Peipei Chen, Xia Gao, Zhi‐Li Huang, Pengyuan Yang , et al. · 2025

Dopaminergic (DA) neurons expressing the dopamine transporter (DAT) play vital roles in physiology and the regulation of mental and neurological disorders. This study investigates spatiotemporal proteomic and transcriptomic changes in DAT+…

Multidimensional Proteomic Landscape Reveals Distinct Activated Pathways Between Human Brain Tumors Open

Shuang Yang, Yongtao Zheng, Chengbin Zhou, Jun Yao, Guoquan Yan , et al. · 2024

Brain metastases (BrMs) and gliomas are two typical human brain tumors with high incidence of mortalities and distinct clinical challenges, yet the understanding of these two types of tumors remains incomplete. Here, a multidimensional pro…

An Alternating Current Electroosmotic Flow‐Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples (Adv. Sci. 6/2024) Open

Huiwen Xiong, Chenxin Zhu, Changhao Dai, Xin Ye, Yuanyuan Li , et al. · 2024

Portable Electrochemiluminescence Microfluidic Devices In article number 2307840, Hui Chen, Huali Shen, Jilie Kong, Xueen Fang, and co‐workers report an ultrasensitive, rapid and portable electrochemiluminescence‐microfluidic (ECL‐M) syste…

An Alternating Current Electroosmotic Flow‐Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples Open

Huiwen Xiong, Chenxin Zhu, Changhao Dai, Xin Ye, Yuanyuan Li , et al. · 2023

Early diagnosis of acute diseases is restricted by the sensitivity and complex process of sample treatment. Here, an ultrasensitive, rapid, and portable electrochemiluminescence‐microfluidic (ECL‐M) system is described via sandwich‐type im…

Pan‐targeted quantification of deep and comprehensive cancer serum proteome improves cancer detection Open

Jiayi Zhang, Anqi Hu, Xuguang Chen, Fenglin Shen, Lei Zhang , et al. · 2023

Serum/plasma proteome analysis can monitor physiological and pathological changes in the human population. In addition to the proteins secreted by tissues, the common blood proteins, including some inherently high‐abundance proteins, also …

Anatomically resolved transcriptome and proteome landscapes reveal disease‐relevant molecular signatures and systematic changes in heart function of end‐stage dilated cardiomyopathy Open

Ling Lin, Shanshan Liu, Zhangwei Chen, Yan Xia, Juanjuan Xie , et al. · 2022

Dilated cardiomyopathy (DCM), as characterized by the left ventricular dilatation and contractile dysfunction, is one of the molecular mechanisms of which are largely unexplored. Here, we profiled the region‐resolved transcriptome and prot…

Correction to An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass Spectrometry Open

Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voß, Ramin Fazel , et al. · 2022

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass SpectrometryYudong GuanYudong GuanMore by Yu…

Cancer Serum Atlas supported precise pan-targeted proteomics enable multi-cancer detection Open

Anqi Hu, Lei Zhang, Zhenxin Wang, Chunyan Yuan, Ling Lin , et al. · 2022

The wide dynamic range of serum proteome restrained discovery of the clinically interested proteins in large cohort studies. Herein, we presented a high-sensitivity, high-throughput and precise pan-targeted serum proteomic strategy for hig…

A sphingolipid-mTORC1 nutrient-sensing pathway regulates animal development by an intestinal peroxisome relocation-based gut-brain crosstalk Open

Na Li, Beilei Hua, Qing Chen, Fukang Teng, Meiyu Ruan , et al. · 2022

Circulating proteomic panels for risk stratification of intracranial aneurysm and its rupture Open

Yueting Xiong, Yongtao Zheng, Yan Yan, Jun Yao, Hebin Liu , et al. · 2022

Matricellular Protein Cilp1 Promotes Myocardial Fibrosis in Response to Myocardial Infarction Open

Qing-jun Zhang, Yu He, Yongnan Li, Huali Shen, Ling Lin , et al. · 2021

Rationale: Cilp1 (cartilage intermediate layer protein 1) is a secreted extracellular matrix protein normally associated with bone and cartilage development. Its function and mechanism of action in adult heart disease remain elusive. Objec…

Precision N-Glycoproteomic Profiling of Murine Peritoneal Macrophages After Different Stimulations Open

Lujie Yang, Tianqi Gong, Huali Shen, Jiangnan Pei, Lei Zhang , et al. · 2021

Macrophages are important immune cells that participate in both innate and adaptive immune responses, such as phagocytosis, recognition of molecular patterns, and activation of the immune response. In this study, murine peritoneal macropha…

Optimized MALDI-TOF MS Strategy for Characterizing Polymers Open

Zhenxin Wang, Quanqing Zhang, Huali Shen, Pengyuan Yang, Xinwen Zhou · 2021

In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plays an essential role in the analysis of polymers. To acquire a more reliable strategy for polymer profiling, we characterized f…

An Integrated Strategy Reveals Complex Glycosylation of Erythropoietin Using Mass Spectrometry Open

Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voß, Ramin Fazel , et al. · 2021

The characterization of therapeutic glycoproteins is challenging due to the structural heterogeneity of the therapeutic protein glycosylation. This study presents an in-depth analytical strategy for glycosylation of first-generation erythr…

A Multi-Omics Study of Human Testis and Epididymis Open

Weimin Zheng, Yang Zhang, Chuanyu Sun, Shengyang Ge, Yifan Tan , et al. · 2021

The human testis and epididymis play critical roles in male fertility, including the spermatogenesis process, sperm storage, and maturation. However, the unique functions of the two organs had not been systematically studied. Herein, we pr…

A lipid-mTORC1 nutrient sensing pathway regulates animal development by peroxisome-derived hormones Open

Na Li, Beilei Hua, Qing Chen, Meiyu Ruan, Mengnan Zhu , et al. · 2021

Animals have developed many signaling mechanisms that alter cellular and developmental programs in response to changes in nutrients and their derived metabolites, many of which remain to be understood. We recently uncovered that glucosylce…

An integrated strategy reveals complex glycosylation of erythropoietin using top-down and bottom-up mass spectrometry Open

Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voß, Ramin Fazel , et al. · 2021

The characterization of glycoproteins, like erythropoietin, is challenging due to the structural micro- and macro-heterogeneity of the protein glycosylation. This study presents an in-depth strategy for glycosylation analysis of a first-ge…

A Sphingolipid-mTORC1 Nutrient-Sensing Machinery Enables Animal Development by Suppression of the Intestinal Peroxisome Relocation, Hormone Secretion and the Gut-Brain Crosstalk Open

Na Li, Beilei Hua, Qing Chen, Meiyu Ruan, Fukang Teng , et al. · 2021

An improved comprehensive strategy for deep and quantitative N-glycomics based on optimization of sample preparation, isotope-based data quality control and quantification, new N-glycan libraries and new algorithms Open

Yudong Guan, Jiaxiang Hu, Weiqian Cao, Wencong Cui, Fan Yang , et al. · 2020

Withdrawal statement “The authors have withdrawn their manuscript because the authors need to re-organize the data and writing, meanwhile more experimental evidence from human cellular N-glycome will be added to support their viewpoints. I…

Quantitative proteomics reveals stage-specific protein regulation of triple negative breast cancer Open

Yuxiang Lin, Ling Lin, Fangmeng Fu, Chuan Wang, Anqi Hu , et al. · 2020

BackgroundTriple negative breast cancer (TNBC) is a heterogeneous disease with more aggressive clinical courses than other subtypes of breast cancer. In this study, we performed high-resolution mass spectrometry-based quantitative proteomi…

Multilayered N-glycoproteomics reveals impaired N-glycosylation promoting Alzheimer’s disease Open

Pan Fang, Juanjuan Xie, Shaoming Sang, Lei Zhang, Mingqi Liu , et al. · 2019

Alzheimer’s disease (AD) is one of the most common neurodegenerative diseases that currently lacks clear pathogenesis and effective treatment. Protein glycosylation is ubiquitous in brain tissue and site-specific analysis of N-glycoproteom…

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer Open

Li Wang, John A. Wrobel, Ling Xie, DongXu Li, Giada Zurlo , et al. · 2018

Erratum: A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline Open

Xiaohui Liu, Weimin Zheng, Wansheng Wang, Huali Shen, Linxiao Liu , et al. · 2018

Correction to: British Journal of Cancer (2017) 117, 1846–1854, doi:10.1038/bjc.2017.365; published online 09 November 2017 Since the publication of this paper, the authors realised an error in the affiliation list. The correct affiliation…

High Efficient and Precise Glycoproteomic Analysis with IntelligentTechnology Open

Pengyuan Yang, Weiqian Cao, Mingqi Liu, Wenfeng Zeng, Chao Liu , et al. · 2018

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline Open

Xiaohui Liu, Weimin Zheng, Wansheng Wang, Huali Shen, Linxiao Liu , et al. · 2017

pGlyco 2.0 enables precision N-glycoproteomics with comprehensive quality control and one-step mass spectrometry for intact glycopeptide identification Open

Mingqi Liu, Wen‐Feng Zeng, Fang Pan, Weiqian Cao, Chao Liu , et al. · 2017

The precise and large-scale identification of intact glycopeptides is a critical step in glycoproteomics. Owing to the complexity of glycosylation, the current overall throughput, data quality and accessibility of intact glycopeptide ident…

Quantitative profiling of glycerophospholipids during mouse and human macrophage differentiation using targeted mass spectrometry Open

Cuiping Zhang, Yi Wang, Fang Wang, Zhenxin Wang, Yu Lu , et al. · 2017

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