Shanjin Huang
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View article: OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility
OsBRK1-mediated phosphorylation of OsPFN2 regulates meiotic spindle-actin assembly and rice fertility Open
Formation of a meiotic spindle structure is crucial for chromosome segregation and fertility in plants. Previous studies have shown that actin decorates spindle microtubules in mammalian oocytes, forming spindle actin, which is indispensab…
View article: Molecular and functional adaption of <i>Arabidopsis</i> villins
Molecular and functional adaption of <i>Arabidopsis</i> villins Open
Summary Villins are versatile, multifunctional actin regulatory proteins. They promote actin stabilization and remodeling mainly via their actin bundling and Ca 2+ ‐dependent severing activities, respectively. Arabidopsis subclass II and I…
View article: Differential sensitivity of ADF isovariants to a pH gradient promotes pollen tube growth
Differential sensitivity of ADF isovariants to a pH gradient promotes pollen tube growth Open
The actin cytoskeleton is one of the targets of the pH gradient in tip-growing cells, but how cytosolic pH regulates the actin cytoskeleton remains largely unknown. We here demonstrate that Arabidopsis ADF7 and ADF10 function optimally at …
View article: Correction: Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development
Correction: Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development Open
[This corrects the article DOI: 10.1371/journal.pbio.3000782.].
View article: GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3
GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3 Open
The actin-rich cortex plays a fundamental role in many cellular processes. Its architecture and molecular composition vary across cell types and physiological states. The full complement of actin assembly factors driving cortex formation a…
View article: Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes
Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes Open
As the stimulus-responsive mediator of actin dynamics, actin-depolymerizing factor (ADF)/cofilin is subject to tight regulation. It is well known that kinase-mediated phosphorylation inactivates ADF/cofilin. Here, however, we found that th…
View article: Actin cytoskeleton in the control of vesicle transport, cytoplasmic organization, and pollen tube tip growth
Actin cytoskeleton in the control of vesicle transport, cytoplasmic organization, and pollen tube tip growth Open
Pollen tubes extend rapidly via tip growth. This process depends on a dynamic actin cytoskeleton, which has been implicated in controlling organelle movements, cytoplasmic streaming, vesicle trafficking, and cytoplasm organization in polle…
View article: The GxcM-Fbp17/RacC-WASP signaling cascade regulates polarized cortex assembly in migrating cells
The GxcM-Fbp17/RacC-WASP signaling cascade regulates polarized cortex assembly in migrating cells Open
The actin-rich cortex plays a fundamental role in many cellular processes. Its architecture and molecular composition vary across cell types and physiological states. The full complement of actin assembly factors driving cortex formation a…
View article: Functional non‐equivalence of pollen<scp>ADF</scp>isovariants in Arabidopsis
Functional non‐equivalence of pollen<span>ADF</span>isovariants in Arabidopsis Open
SUMMARY ADF/cofilin is a central regulator of actin dynamics. We previously demonstrated that two closely related Arabidopsis class IIa ADF isovariants, ADF7 and ADF10, are involved in the enhancement of actin turnover in pollen, but wheth…
View article: Phosphatidylinositol 3-phosphate regulates SCAB1-mediated F-actin reorganization during stomatal closure in Arabidopsis
Phosphatidylinositol 3-phosphate regulates SCAB1-mediated F-actin reorganization during stomatal closure in Arabidopsis Open
Stomatal movement is critical for plant responses to environmental changes and is regulated by the important signaling molecule phosphatidylinositol 3-phosphate (PI3P). However, the molecular mechanism underlying this process is not well u…
View article: Actin filament debranching regulates cell polarity during cell migration and asymmetric cell division
Actin filament debranching regulates cell polarity during cell migration and asymmetric cell division Open
Significance We find that the coronin family protein POD-1 debranches the actin network in vitro. Our results reveal that POD-1 regulates actin organization and cell polarity in migrating neuroblasts and one-cell−stage embryos of C. elegan…
View article: An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins
An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins Open
Cell-to-cell communication in plants is mediated by plasmodesmata (PD) whose permeability is tightly regulated during plant growth and development. The actin cytoskeleton has been implicated in regulating the permeability of PD, but the un…
View article: Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes
Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes Open
In flowering plants, sexual reproduction involves a double fertilization event, which is facilitated by the delivery of two non-motile sperm cells to the ovule by the pollen tube. Pollen tube growth occurs exclusively at the tip and is ext…
View article: Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development
Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development Open
Tight regulation of gene transcription and mRNA splicing is essential for plant growth and development. Here we demonstrate that a plant-specific protein, EMBRYO DEFECTIVE 1579 (EMB1579), controls multiple growth and developmental processe…
View article: GLABRA2 Regulates Actin Bundling Protein VILLIN1 in Root Hair Growth in Response to Osmotic Stress
GLABRA2 Regulates Actin Bundling Protein VILLIN1 in Root Hair Growth in Response to Osmotic Stress Open
Actin binding proteins and transcription factors are essential in regulating plant root hair growth in response to various environmental stresses; however, the interaction between these two factors in regulating root hair growth remains po…
View article: PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins Open
PSGL-1 has recently been identified as an HIV restriction factor that inhibits HIV DNA synthesis and more potently, virion infectivity. But the underlying mechanisms of these inhibitions are unknown. Here we show that PSGL-1 directly binds…
View article: Villin controls the formation and enlargement of punctate actin foci in pollen tubes
Villin controls the formation and enlargement of punctate actin foci in pollen tubes Open
Self-incompatibility (SI) in the poppy Papaver rhoeas triggers dramatic alterations in actin within pollen tubes. However, how these actin alterations are mechanistically achieved remains largely unexplored. Here, we used treatment with th…
View article: Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes Open
Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its sp…
View article: A SAC Phosphoinositide Phosphatase Controls Rice Development via Hydrolyzing PI4P and PI(4,5)P<sub>2</sub>
A SAC Phosphoinositide Phosphatase Controls Rice Development via Hydrolyzing PI4P and PI(4,5)P<sub>2</sub> Open
Phosphoinositides (PIs) as regulatory membrane lipids play essential roles in multiple cellular processes. Although the exact molecular targets of PI-dependent modulation remain largely elusive, the effects of disturbed PI metabolism could…
View article: A SAC phosphoinositide phosphatase controls rice development via hydrolyzing phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate
A SAC phosphoinositide phosphatase controls rice development via hydrolyzing phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate Open
Phosphoinositides (PIs) as regulatory membrane lipids play essential roles in multiple cellular processes. Although the exact molecular targets of PIs-dependent modulation remain largely elusive, the effects of disturbed PIs metabolism cou…
View article: Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells
Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells Open
The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Eluc…