Huiqiang Lou
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View article: S-phase PARylation of microprotein RSMC enhances the function of Sororin in sister chromatid cohesion
S-phase PARylation of microprotein RSMC enhances the function of Sororin in sister chromatid cohesion Open
Sororin is essential for establishing sister chromatid cohesion concurrently with DNA replication in metazoans. Although acetylation of the cohesin subunit SMC3 by ESCO1/2 is necessary for Sororin recruitment, it is by itself not sufficien…
View article: S-CDK-regulated bipartite interaction of Mcm10 with MCM is essential for DNA replication
S-CDK-regulated bipartite interaction of Mcm10 with MCM is essential for DNA replication Open
Mcm10 plays an essential role in the activation of replicative helicase CMG through the cell cycle-regulated interaction with the prototype MCM double hexamer in Saccharomyces cerevisiae . In this study, we reported that Mcm10 is phosphory…
View article: H3T11 phosphorylation by CKII is required for heterochromatin formation in <i>Neurospora</i>
H3T11 phosphorylation by CKII is required for heterochromatin formation in <i>Neurospora</i> Open
Heterochromatin is a key feature of eukaryotic genomes and is crucial for maintaining genomic stability. In fission yeast, heterochromatin nucleation is mainly mediated by DNA-binding proteins or the RNA interference (RNAi) pathway. In the…
View article: Symmetric control of sister chromatid cohesion establishment
Symmetric control of sister chromatid cohesion establishment Open
Besides entrapping sister chromatids, cohesin drives other high-order chromosomal structural dynamics like looping, compartmentalization and condensation. ESCO2 acetylates a subset of cohesin so that cohesion must be established and only b…
View article: Phosphorylation of the LCB1 subunit of <i>Arabidopsis</i> serine palmitoyltransferase stimulates its activity and modulates sphingolipid biosynthesis
Phosphorylation of the LCB1 subunit of <i>Arabidopsis</i> serine palmitoyltransferase stimulates its activity and modulates sphingolipid biosynthesis Open
Sphingolipids are the structural components of membrane lipid bilayers and act as signaling molecules in many cellular processes. Serine palmitoyltransferase (SPT) is the first committed and rate‐limiting enzyme in the de novo sphingolipid…
View article: Transcription-independent hold of the G1/S transition is exploited to cope with DNA replication stress
Transcription-independent hold of the G1/S transition is exploited to cope with DNA replication stress Open
RB1 (retinoblastoma) members control the G1/S commitment as transcriptional repressors in eukaryotic cells. Here we uncover that an extra copy of RB1 equivalent ( WHI7 or WHI5 ) is sufficient to bypass the indispensability of the central g…
View article: HDA-2-Containing Complex Is Required for Activation of <i>Catalase-3</i> Expression in Neurospora crassa
HDA-2-Containing Complex Is Required for Activation of <i>Catalase-3</i> Expression in Neurospora crassa Open
Clearance of reactive oxygen species (ROS) is critical to the survival of aerobic organisms. In the model filamentous fungus Neurospora crassa , catalase-3 ( cat-3 ) expression is activated in response to H 2 O 2 -induced ROS stress.
View article: Editorial: DNA Replication Stress and Cell Fate
Editorial: DNA Replication Stress and Cell Fate Open
International audience
View article: Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan
Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan Open
Xylanases with high specific activity has been focused with great interest as a useful enzyme in biomass utilization. The production of recombinant GH11 xylanase (MYCTH_56237) from Myceliophthora thermophila has been improved through N-ter…
View article: Stochasticity Triggers Activation of the S-phase Checkpoint Pathway in Budding Yeast
Stochasticity Triggers Activation of the S-phase Checkpoint Pathway in Budding Yeast Open
In a complex and ever-changing environment, various signal transduction pathways mediate outside signals and stress to a living cell and its intracellular responses. Eukaryotic cells utilize the DNA synthesis phase (S-phase) checkpoint to …
View article: Phosphorylation of LCB1 subunit of serine palmitoyltransferase stimulates its activity and modulates sphingolipid biosynthesis in Arabidopsis
Phosphorylation of LCB1 subunit of serine palmitoyltransferase stimulates its activity and modulates sphingolipid biosynthesis in Arabidopsis Open
Sphingolipids are the structural elements for membrane lipid bilayers and the signal molecules for many cellular processes. Serine palmitoyltransferase (SPT) is the first committed and rate-limiting enzyme in the de novo sphingolipids bios…
View article: Synthetic lethality between TP53 and ENDOD1
Synthetic lethality between TP53 and ENDOD1 Open
The atypical nuclease ENDOD1 functions with cGAS-STING in innate immunity. Here we identify a previously uncharacterized ENDOD1 function in DNA repair. ENDOD1 is enriched in the nucleus following H 2 O 2 treatment and ENDOD1 -/- cells show…
View article: Mthfd2 Modulates Mitochondrial Function and DNA Repair to Maintain the Pluripotency of Mouse Stem Cells
Mthfd2 Modulates Mitochondrial Function and DNA Repair to Maintain the Pluripotency of Mouse Stem Cells Open
The pluripotency of stem cells determines their developmental potential. While the pluripotency states of pluripotent stem cells are variable and interconvertible, the mechanisms underlying the acquisition and maintenance of pluripotency r…
View article: Post-Translational Modifications Aid Archaeal Survival
Post-Translational Modifications Aid Archaeal Survival Open
Since the pioneering work of Carl Woese, Archaea have fascinated biologists of almost all areas given their unique evolutionary status, wide distribution, high diversity, and ability to grow in special environments. Archaea often thrive in…
View article: The acetyltransferase Eco1 elicits cohesin dimerization during S phase
The acetyltransferase Eco1 elicits cohesin dimerization during S phase Open
Sister chromatid cohesion is established by Eco1 in S phase. Nevertheless, the exact consequence of Eco1-catalyzed acetylation is unknown, and the cohesive state remains highly controversial. Here we show that self-interactions of cohesin …
View article: The Emerging Roles of Fox Family Transcription Factors in Chromosome Replication, Organization, and Genome Stability
The Emerging Roles of Fox Family Transcription Factors in Chromosome Replication, Organization, and Genome Stability Open
The forkhead box (Fox) transcription factors (TFs) are widespread from yeast to humans. Their mutations and dysregulation have been linked to a broad spectrum of malignant neoplasias. They are known as critical players in DNA repair, metab…
View article: Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats Open
The S-phase checkpoint plays an essential role in regulation of the ribonucleotide reductase (RNR) activity to maintain the dNTP pools. How eukaryotic cells respond appropriately to different levels of replication threats remains elusive. …
View article: Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats Open
The S-phase checkpoint plays an essential role in regulation of the ribonucleotide reductase (RNR) activity to maintain the dNTP pools. How eukaryotic cells respond appropriately to different levels of replication threats remains elusive. …
View article: Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2
Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2 Open
Cohesin acetyltransferases ESCO1 and ESCO2 play a vital role in establishing sister chromatid cohesion. How ESCO1 and ESCO2 are controlled in a DNA replication-coupled manner remains unclear in higher eukaryotes. Here we show a critical ro…
View article: Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2
Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2 Open
Cohesin acetyltransferases Esco1 and Esco2 play a vital role in establishing sister chromatid cohesion. How Esco1 and Esco2 are controlled to achieve this in a DNA replication-coupled manner remains unclear in higher eukaryotes. Here we sh…
View article: Flexibly-oriented double Cdc45-MCM-GINS intermediates during eukaryotic replicative helicase maturation
Flexibly-oriented double Cdc45-MCM-GINS intermediates during eukaryotic replicative helicase maturation Open
The core of the eukaryotic helicase MCM is loaded as an inactive double hexamer (DH). How it is assembled into two active Cdc45-MCM-GINS (CMG) helicases remains elusive. Here, we report that at the onset of S phase, both Cdc45 and GINS are…
View article: Characterization of Two Endo-β-1, 4-Xylanases from Myceliophthora thermophila and Their Saccharification Efficiencies, Synergistic with Commercial Cellulase
Characterization of Two Endo-β-1, 4-Xylanases from Myceliophthora thermophila and Their Saccharification Efficiencies, Synergistic with Commercial Cellulase Open
The xylanases with high specific activity and resistance to harsh conditions are of high practical value for biomass utilization. In the present study, two new GH11 xylanase genes, MYCTH_56237 and MYCTH_49824, have been cloned from thermop…
View article: Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing
Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing Open
Initiation of eukaryotic chromosome replication follows a spatiotemporal program. The current model suggests that replication origins compete for a limited pool of initiation factors. However, it remains to be answered how these limiting f…
View article: The DNA Pol <b>ϵ</b> stimulatory activity of Mrc1 is modulated by phosphorylation
The DNA Pol <b>ϵ</b> stimulatory activity of Mrc1 is modulated by phosphorylation Open
DNA replication checkpoint (Mec1-Mrc1-Rad53 in budding yeast) is an evolutionarily conserved surveillance system to ensure proper DNA replication and genome stability in all eukaryotes. Compared to its well-known function as a mediator of …