Ian R. Monk
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View article: Regulation of airway fumarate by host and pathogen promotes Staphylococcus aureus pneumonia
Regulation of airway fumarate by host and pathogen promotes Staphylococcus aureus pneumonia Open
View article: Global analysis of the genomic diversity, antimicrobial resistance and potential vaccine candidates carried by the major global bovine pathogen Streptococcus uberis
Global analysis of the genomic diversity, antimicrobial resistance and potential vaccine candidates carried by the major global bovine pathogen Streptococcus uberis Open
Streptococcus uberis is a leading cause of bovine mastitis, resulting in large economic losses and welfare issues for affected cows. However, relatively little is known about the global distribution and emergence of bovine mastitis-causing…
View article: Phosphorylation by the PknB serine/threonine kinase stimulates dimer formation by WalR
Phosphorylation by the PknB serine/threonine kinase stimulates dimer formation by WalR Open
WalKR is a two-component system that regulates cell wall homeostasis and other processes in low-GC Gram-positive bacteria. It is essential for viability in Staphylococcus aureus and regulates genes that encode the autolysins and other crit…
View article: Activation of the <i>Staphylococcus aureus</i> intramembrane sensing histidine kinase SaeS via intramembrane interaction with the bacterially encoded small protein ScrA
Activation of the <i>Staphylococcus aureus</i> intramembrane sensing histidine kinase SaeS via intramembrane interaction with the bacterially encoded small protein ScrA Open
Two-component systems represent a fundamental sense-response mechanism in bacterial cells and often play important roles in critical processes such as quorum sensing, antibiotic tolerance, and virulence. Much has been learned about the rol…
View article: Comparative and functional genomic analysis of foreign DNA defense mechanisms in <i>Enterococcus faecium</i>
Comparative and functional genomic analysis of foreign DNA defense mechanisms in <i>Enterococcus faecium</i> Open
Enterococcus faecium is notoriously difficult to study genetically due to the poor understanding of barriers preventing foreign DNA uptake, such as the proteins that modify type I restriction modification (RM) system activity. Here, we com…
View article: Transposon-directed insertion-site sequencing (TraDIS) analysis of <i>Enterococcus faecium</i> using nanopore sequencing and a WebAssembly analysis platform
Transposon-directed insertion-site sequencing (TraDIS) analysis of <i>Enterococcus faecium</i> using nanopore sequencing and a WebAssembly analysis platform Open
Vancomycin-resistant Enterococcus faecium (VREfm) are healthcare-associated opportunistic pathogens of global significance. Genetic tools are needed to understand the molecular basis for VREfm clinically relevant phenotypes, such as persis…
View article: Are reads required? High-precision variant calling from bacterial genome assemblies
Are reads required? High-precision variant calling from bacterial genome assemblies Open
Accurate nucleotide variant calling is essential in microbial genomics, particularly for outbreak tracking and phylogenetics. This study evaluates variant calls derived from genome assemblies compared to traditional read-based variant-call…
View article: Are reads required? High-precision variant calling from bacterial genome assemblies
Are reads required? High-precision variant calling from bacterial genome assemblies Open
Accurate nucleotide variant calling is essential in microbial genomics, particularly for outbreak tracking and phylogenetics. This study evaluates variant calls derived from genome assemblies compared to traditional read-based variant-call…
View article: Characterisation of <i>in vitro</i> resistance selection against second-/last-line antibiotics in methicillin-resistant <i>Staphylococcus aureus</i> ATCC 43300 strain
Characterisation of <i>in vitro</i> resistance selection against second-/last-line antibiotics in methicillin-resistant <i>Staphylococcus aureus</i> ATCC 43300 strain Open
Background and objectives The increasing occurrence of MRSA clinical isolates harbouring reduced susceptibility to mainstay antibiotics has escalated the use of second and last line antibiotics. Hence, it is critical to evaluate the likeli…
View article: Regulation of airway fumarate by host and pathogen promotes S. <i>aureus</i> pneumonia
Regulation of airway fumarate by host and pathogen promotes S. <i>aureus</i> pneumonia Open
Staphylococcus aureus is a leading cause of healthcare-associated pneumonia, contributing significantly to morbidity and mortality worldwide. As a ubiquitous colonizer of the upper respiratory tract, S. aureus must undergo substantial meta…
View article: Are reads required? High-precision variant calling from bacterial genome assemblies
Are reads required? High-precision variant calling from bacterial genome assemblies Open
Accurate nucleotide variant calling is essential in microbial genomics, particularly for outbreak tracking and phylogenetics. This study evaluates variant calls derived from genome assemblies compared to traditional read-based variant-call…
View article: Transposon-directed insertion-site sequencing (TraDIS) analysis of <i>Enterococcus faecium</i> using nanopore sequencing and a WebAssembly analysis platform
Transposon-directed insertion-site sequencing (TraDIS) analysis of <i>Enterococcus faecium</i> using nanopore sequencing and a WebAssembly analysis platform Open
Vancomycin-resistant Enterococcus faecium (VREfm) are healthcare-associated opportunistic pathogens of global significance. Genetic tools are needed to understand the molecular basis for VREfm clinically relevant phenotypes, such as persis…
View article: Are reads required? High-precision variant calling from bacterial genome assemblies
Are reads required? High-precision variant calling from bacterial genome assemblies Open
1. Abstract Accurate nucleotide variant calling is essential in microbial genomics, particularly for outbreak tracking and phylogenetics. This study evaluates variant calls derived from genome assemblies compared to traditional read-based …
View article: Characterisation of <i>in vitro</i> resistance selection against second-/last-line antibiotics in methicillin-resistant <i>Staphylococcus aureus</i>
Characterisation of <i>in vitro</i> resistance selection against second-/last-line antibiotics in methicillin-resistant <i>Staphylococcus aureus</i> Open
SYNOPSIS Background The increasing occurrence of MRSA clinical isolates harbouring reduced susceptibility to mainstay antibiotics has escalated the use of second and last line antibiotics. Hence, it is critical to evaluate the likelihood o…
View article: Staphylococcal superantigens evoke temporary and reversible T cell anergy, but fail to block the development of a bacterium specific cellular immune response
Staphylococcal superantigens evoke temporary and reversible T cell anergy, but fail to block the development of a bacterium specific cellular immune response Open
Superantigens (sAgs) are bacterial virulence factors that induce a state of immune hyperactivation by forming a bridge between certain subsets of T cell receptor (TCR) β chains on T lymphocytes, and class II major histocompatibility comple…
View article: Rifaximin prophylaxis causes resistance to the last-resort antibiotic daptomycin
Rifaximin prophylaxis causes resistance to the last-resort antibiotic daptomycin Open
View article: Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format
Optimization of Reverse Transcription Loop-Mediated Isothermal Amplification for In Situ Detection of SARS-CoV-2 in a Micro-Air-Filtration Device Format Open
The Coronavirus disease 2019 (COVID-19) pandemic has supercharged innovation in the field of molecular diagnostics and led to the exploration of systems that permit the autonomous identification of airborne infectious agents. Airborne viru…
View article: Marine sponge microbe provides insights into evolution and virulence of the tubercle bacillus
Marine sponge microbe provides insights into evolution and virulence of the tubercle bacillus Open
Reconstructing the evolutionary origins of Mycobacterium tuberculosis , the causative agent of human tuberculosis, has helped identify bacterial factors that have led to the tubercle bacillus becoming such a formidable human pathogen. Here…
View article: Staphylococcus aureus adapts to exploit collagen-derived proline during chronic infection
Staphylococcus aureus adapts to exploit collagen-derived proline during chronic infection Open
Staphylococcus aureus is a pulmonary pathogen associated with substantial human morbidity and mortality. As vaccines targeting virulence determinants have failed to be protective in humans, other factors are likely involved in pathogenesis…
View article: Polyclonal but not monoclonal circulating memory CD4+ T cells attenuate the severity of Staphylococcus aureus bacteremia
Polyclonal but not monoclonal circulating memory CD4+ T cells attenuate the severity of Staphylococcus aureus bacteremia Open
Staphylococcus aureus bacteremia causes significant morbidity and mortality. Treatment of staphylococcal infections is hindered by widespread antibiotic resistance, and attempts to develop an S. aureus vaccine have failed. Improved S. aure…
View article: Emergence and clonal expansion of a qacA-harbouring sequence type 45 lineage of methicillin-resistant Staphylococcus aureus
Emergence and clonal expansion of a qacA-harbouring sequence type 45 lineage of methicillin-resistant Staphylococcus aureus Open
The past decade has seen an increase in the prevalence of sequence type (ST) 45 methicillin-resistant Staphylococcus aureus (MRSA), yet the underlying drivers for its emergence and spread remain unclear. To better understand the worldwide …
View article: A marine sponge-associated mycobacterium closely related to<i>Mycobacterium tuberculosis</i>
A marine sponge-associated mycobacterium closely related to<i>Mycobacterium tuberculosis</i> Open
Reconstructing the evolutionary origins of Mycobacterium tuberculosis , the causative agent of human tuberculosis, has helped identify bacterial factors that have led to the tubercle bacillus becoming such a formidable human pathogen. Here…
View article: The two-component system WalKR provides an essential link between cell wall homeostasis and DNA replication in <i>Staphylococcus aureus</i>
The two-component system WalKR provides an essential link between cell wall homeostasis and DNA replication in <i>Staphylococcus aureus</i> Open
Among the 16 two-component systems in the opportunistic human pathogen Staphylococcus aureus , only WalKR is essential. Like the orthologous systems in other Bacillota, S. aureus WalKR controls autolysins involved in peptidoglycan remodeli…
View article: Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria
Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria Open
Even in the setting of optimal resuscitation in high-income countries severe sepsis and septic shock have a mortality of 20–40%, with antibiotic resistance dramatically increasing this mortality risk. To develop a reference dataset enablin…
View article: Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus
Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus Open
Staphylococcus aureus is a human pathogen that upon invasion of a host encounters stresses, such as nutritional restriction. The bacteria respond by switching on a signaling cascade controlled by the nucleotides (p)ppGpp.
View article: Rifaximin prophylaxis causes resistance to the last-resort antibiotic daptomycin
Rifaximin prophylaxis causes resistance to the last-resort antibiotic daptomycin Open
Multidrug-resistant bacterial pathogens like vancomycin-resistant Enterococcus faecium (VREfm) are a critical threat to human health. Daptomycin is a last-resort antibiotic for VREfm infections with a novel mode-of-action, but for which re…
View article: The two-component regulator WalKR provides an essential link between cell wall homeostasis with DNA replication in<i>Staphylococcus aureus</i>
The two-component regulator WalKR provides an essential link between cell wall homeostasis with DNA replication in<i>Staphylococcus aureus</i> Open
Among the 16 two-component systems (TCSs) in the opportunistic human pathogen Staphylococcus aureus , only WalKR is essential. Like orthologous systems in other Bacillota, S. aureus WalKR controls autolysins involved in peptidoglycan remod…
View article: Staphylococcus aureus stimulates neutrophil itaconate production that suppresses the oxidative burst
Staphylococcus aureus stimulates neutrophil itaconate production that suppresses the oxidative burst Open
View article: Staphylococcus aureus host interactions and adaptation
Staphylococcus aureus host interactions and adaptation Open
View article: Improved Genome Sequence of Australian Methicillin-Resistant Staphylococcus aureus Strain JKD6159
Improved Genome Sequence of Australian Methicillin-Resistant Staphylococcus aureus Strain JKD6159 Open
Staphylococcus aureus strain JKD6159 represents a prominent community-acquired methicillin-resistant S. aureus (MRSA) clone in Australia. Here, we report an improved assembly of the original S. aureus JKD6159 genome sequence. By using deep…