Explanipedia

Helix sliding in the stalk coiled coil of dynein couples ATPase and microtubule binding Open

Takahide Kon, Kenji Imamula, A. J. Roberts, Reiko Ohkura, Peter J. Knight , et al. · 2009

Structure and Functional Role of Dynein's Microtubule-Binding Domain Open

Andrew P. Carter, Joan E. Garbarino, Elizabeth M. Wilson-Kubalek, Wesley E. Shipley, Carol Cho , et al. · 2008

Dynein motors move various cargos along microtubules within the cytoplasm and power the beating of cilia and flagella. An unusual feature of dynein is that its microtubule-binding domain (MTBD) is separated from its ring-shaped AAA+ adenos…

The Genome of the Sea Urchin <i>Strongylocentrotus purpuratus</i> Open

Erica Sodergren, George M. Weinstock, Eric H. Davidson, R. Andrew Cameron, Richard A. Gibbs , et al. · 2006

We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus , a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial …

Analysis of cytoskeletal and motility proteins in the sea urchin genome assembly Open

Robert L. Morris, Matthew P. Hoffman, Robert A. Obar, S. Shawn McCafferty, I. R. Gibbons , et al. · 2006

Cytoplasmic dynein nomenclature Open

K. Kevin Pfister, Elizabeth Fisher, I. R. Gibbons, Thomas S. Hays, Erika L.F. Holzbaur , et al. · 2005

A variety of names has been used in the literature for the subunits of cytoplasmic dynein complexes. Thus, there is a strong need for a more definitive consensus statement on nomenclature. This is especially important for mammalian cytopla…

The Affinity of the Dynein Microtubule-binding Domain Is Modulated by the Conformation of Its Coiled-coil Stalk Open

I. R. Gibbons, Joan E. Garbarino, C. E. Tan, Samara L. Reck‐Peterson, Ronald D. Vale , et al. · 2005

The microtubule-binding domain (MTBD) of dynein is separated from the AAA (ATPase with any other activity) core of the motor by an approximately 15-nm stalk that is predicted to consist of an antiparallel coiled coil. However, the structur…

Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein Open

Joan E. Garbarino, I. R. Gibbons · 2002

The highly conserved structure of midasin in eukaryotes, taken in conjunction with its nuclear localization in yeast, suggests that midasin may function as a nuclear chaperone and be involved in the assembly/disassembly of macromolecular c…

Model for the Motor Component of Dynein Heavy Chain Based on Homology to the AAA Family of Oligomeric ATPases Open

Gábor Mócz, I. R. Gibbons · 2001

Rotation of the Central Pair Microtubules in Eukaryotic Flagella Open

Charlotte K. Omoto, I. R. Gibbons, Ritsu Kamiya, Chikako Shingyoji, Keiichi Takahashi , et al. · 1999

The Role of Dynein in Microtubule-based Motility. Open

I. R. Gibbons · 1996

Dyneins are high molecular weight ATPases that function as microtubule-based molecular motors. The axonemal dyneins, discovered 30 years ago, are responsible for the beating movement of cilia and sperm flagella, which they generate by prod…

Masthead Open

Robert J. Allen, B. R. Brinkley, Robert D. Goldman, Jeremy S. Hyams, Ritsu Kamiya , et al. · 1996

Masthead Open

Robert J. Allen, B. R. Brinkley, Robert D. Goldman, Jeremy S. Hyams, Ritsu Kamiya , et al. · 1996

Effect of Beat Frequency on the Velocity of Microtubule Sliding in Reactivated Sea Urchin Sperm Flagella Under Imposed Head Vibration Open

Chikako Shingyoji, Kenjiro Yoshimura, Dan Eshel, Keiichi Takahashi, I. R. Gibbons · 1995

The heads of demembranated spermatozoa of the sea urchin Tripneustes gratilla, reactivated at different concentrations of ATP, were held by suction in the tip of a micropipette and vibrated laterally with respect to the head axis. This imp…

Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation. Open

W.S. Saunders, Douglas Koshland, Dan Eshel, I. R. Gibbons, M. Andrew Hoyt · 1995

The Saccharomyces cerevisiae kinesin-related gene products Cin8p and Kip1p function to assemble the bipolar mitotic spindle. The cytoplasmic dynein heavy chain homologue Dyn1p (also known as Dhc1p) participates in proper cellular positioni…

Negative complementation in aspartate transcarbamylase. Analysis of hybrid enzyme molecules containing different arrangements of polypeptide chains from wild-type and inactive mutant catalytic subunits. Open

Edward Eisenstein, Meekyung Han, Tae-Wook Woo, James M. Ritchey, I. R. Gibbons , et al. · 1992

A comprehensive set of hybrid molecules of aspartate transcarbamylase (ATCase) from Escherichia coli has been constructed of wild-type and mutationally altered catalytic chains. The mutant enzymes that were virtually devoid of activity con…

Masthead Open

Garth L. Nicolson, W. J. Pledger, Gary Stein, Richard Anderson, Mlna Bissell , et al. · 1991

A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library. Open

I. R. Gibbons, David J. Asai, N. Ching, Gregory J. Dolecki, Gábor Mócz , et al. · 1991

A procedure that uses the PCR to make rapid successive steps through a random-primed cDNA library has been developed to provide a method for sequencing very long genes that are difficult to obtain as a single clone. In each successive step…

Masthead Open

Y Forrnedy, Garth L. Nicolson, W. J. Pledger, Gary Stein, Richard Anderson , et al. · 1991

Masthead Open

Richard Anderson, Mlna Bissell, Judith Camplsl, Kermit L. Carraway, Moses V. Chao , et al. · 1991

Transient behavior of sea urchin sperm flagella following an abrupt change in beat frequency Open

Dan Eshel, Chikako Shingyoji, Kenjiro Yoshimura, Barbara Gibbons, I. R. Gibbons , et al. · 1990

Within the approximate range of 30–80 Hz, the flagellar beat frequency of a sea urchin sperm held by its head in the tip of a micropipet is governed by the vibration frequency of the micropipet. We have imposed abrupt changes in flagellar …

Iron(III)-mediated photolysis of outer arm dynein ATPase from sea urchin sperm flagella. Open

Gábor Mócz, I. R. Gibbons · 1990

Irradiation of outer arm dynein ATPase from sea urchin sperm tail flagella at 365-410 nm in the presence of Fe(III)-gluconate complex and ATP produces photolytic cleavage at two distinct sites on the beta heavy chain, located approximately…

PSM volume 19 issue 4 Cover and Front matter Open

Michael Shepherd, I. R. Gibbons, K Rawnsley, G Ashcroft, David Broadbent , et al. · 1989

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Masthead Open

Max M. Burger, Ditori Al, Ralph Bradshaw, Dennis D. Cunningham, Elliot Elson , et al. · 1989

Dynein ATPases as microtubule motors. Open

I. R. Gibbons · 1988

PSM volume 18 issue 3 Cover and Front matter Open

Michael Shepherd, I. R. Gibbons, K Rawnsley, G Ashcroft, Robert A. Hinde , et al. · 1988

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A map of photolytic and tryptic cleavage sites on the beta heavy chain of dynein ATPase from sea urchin sperm flagella. Open

Gábor Mócz, Wei‐Jen Tang, I. R. Gibbons · 1988

NH2-terminal analysis of the alpha and beta heavy chain polypeptides (Mr greater than 400,000) from the outer arm dynein of sea urchin sperm flagella, compared with that of the 230,000- and 200,000-Mr peptides formed upon photocleavage of …

PSM volume 18 issue 1 Cover and Front matter Open

Michael Shepherd, I. R. Gibbons, K Rawnsley, G Ashcroft, Robert A. Hinde , et al. · 1988

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

New jobs for dynein ATPases Open

I. R. Gibbons · 1987

Photosensitized cleavage of dynein heavy chains. Cleavage at the V2 site by irradiation at 365 NM in the presence of oligovanadate. Open

I. R. Gibbons, A. Lee-Eiford, Gábor Mócz, C. A. Phillipson, Wei‐Jen Tang , et al. · 1987

Irradiation of the outer-arm dynein ATPase from sea urchin sperm flagella at 365 nm in the presence of 50-200 microM vanadate (Vi) and 1 mM manganese acetate, in the absence of ATP, cleaves the alpha and beta heavy chains at a specific sit…

Use of liposome encapsulation in a combined single-liquid reagent for homogeneous enzyme immunoassay. Open

Edwin F. Ullman, Thomas Tarnowski, Philip L. Felgner, I. R. Gibbons · 1987

A technique has been developed to permit mutually reactive macromolecular reagents used in immunoassays to be combined without premature reaction. A conjugate of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and theophylline has been enc…

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