Explanipedia

Development of a BRET based chloride biosensor for high throughput screening of KCC2 modulators Open

Charles S. Lay, E. Diamantopoulou, Katharina Duerr, Idlir Liko, Steven J. Charlton · 2025

The generation of action potentials in neuronal cells and many other physiological processes involve the transport of chloride ions. Whilst there have been advances in chloride imaging techniques utilizing FRET biosensors, there is a lack …

The evolving role of hydrogen/deuterium exchange mass spectrometry in early-stage drug discovery Open

Srinath Krishnamurthy, Maria Musgaard, Benjamin G. Tehan, Ali Jazayeri, Idlir Liko · 2025

Protein function relies on protein dynamics and therefore dynamical information can be crucial for drug discovery of challenging protein targets. Assessing protein dynamics experimentally has traditionally been nontrivial. However, amide h…

Defining proteoform-specific interactions for drug targeting in a native cell signalling environment Open

Corinne A. Lutomski, Jack L. Bennett, Tarick J. El‐Baba, Di Wu, Joshua D. Hinkle , et al. · 2025

Infrared Multiphoton Dissociation Enables Top‐Down Characterization of Membrane Protein Complexes and G Protein‐Coupled Receptors Open

Corinne A. Lutomski, Tarick J. El‐Baba, Joshua D. Hinkle, Idlir Liko, Jack L. Bennett , et al. · 2023

Membrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires stabilization in detergent micelles that are removed prior to analysis via collisional activation. There is howeve…

Infrared Multiphoton Dissociation Enables Top‐Down Characterization of Membrane Protein Complexes and G Protein‐Coupled Receptors Open

Corinne A. Lutomski, Tarick J. El‐Baba, Joshua D. Hinkle, Idlir Liko, Jack L. Bennett , et al. · 2023

Membrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires stabilization in detergent micelles that are removed prior to analysis via collisional activation. There is howeve…

Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor Open

Hsin‐Yung Yen, Idlir Liko, Wanling Song, Parth Kapoor, Fernando Almeida , et al. · 2022

Non-ionic hybrid detergents for protein delipidation Open

Leonhard H. Urner, Idlir Liko, Kevin Pagel, Rainer Haag, Carol V. Robinson · 2022

Non-ionic detergents are important tools for the investigation of interactions between membrane proteins and lipid membranes. Recent studies led to the question as to whether the ability to capture protein-lipid interactions depends on the…

Electrospray ionization of native membrane proteins proceeds <i>via</i> a charge equilibration step Open

Hsin‐Yung Yen, Mia Abramsson, Mark T. Agasid, Dilraj Lama, Joseph Gault , et al. · 2022

The electrospray ionization mechanism contributes to preserving the structures and interactions of membrane protein complexes in native mass spectrometry.

NaViA: a program for the visual analysis of complex mass spectra Open

Daniel Quetschlich, Tim K. Esser, Thomas D. Newport, Francesco Fiorentino, Denis Shutin , et al. · 2021

Motivation Native mass spectrometry is now a well-established method for the investigation of protein complexes, specifically their subunit stoichiometry and ligand binding properties. Recent advances allowing the analysis of complex mixtu…

Phospho‐regulation, nucleotide binding and ion access control in potassium‐chloride cotransporters Open

Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence Tremblay , et al. · 2021

SLC12A4/SLC12A6; A Target Enabling Package Open

Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence Tremblay , et al. · 2020

KCC1 (SLC12A4) and KCC3 (SLC12A6) are co-transporters of potassium and chloride, and members of cation-chloride co-transporter (CCC; or Solute Carrier 12) family. They regulate chloride level and cell volume via export of potassium and chl…

SLC12A4/SLC12A6; A Target Enabling Package Open

Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence Tremblay , et al. · 2020

KCC1 (SLC12A4) and KCC3 (SLC12A6) are co-transporters of potassium and chloride, and members of cation-chloride co-transporter (CCC; or Solute Carrier 12) family. They regulate chloride level and cell volume via export of potassium and chl…

SLC12A4/SLC12A6; A Target Enabling Package Open

Gamma Chi, Rebecca Ebenhoch, Henry Man, Haiping Tang, Laurence Tremblay , et al. · 2020

KCC1 (SLC12A4) and KCC3 (SLC12A6) are co-transporters of potassium and chloride, and members of cation-chloride co-transporter (CCC; or Solute Carrier 12) family. They regulate chloride level and cell volume via export of potassium and chl…

Dendritic Oligoglycerol Regioisomer Mixtures and Their Utility for Membrane Protein Research Open

Leonhard H. Urner, Katharina Goltsche, Marleen Selent, Idlir Liko, Marc‐Philip Schweder , et al. · 2020

Dendrons are an important class of macromolecules that can be used for a broad range of applications. Recent studies have indicated that mixtures of oligoglycerol detergent (OGD) regioisomers are superior to individual regioisomers for pro…

Correlating Glycoforms of DC‐SIGN with Stability Using a Combination of Enzymatic Digestion and Ion Mobility Mass Spectrometry Open

Hsin‐Yung Yen, Idlir Liko, Joseph Gault, Di Wu, Weston B. Struwe , et al. · 2020

The immune scavenger protein DC‐SIGN interacts with glycosylated proteins and has a putative role in facilitating viral infection. How these recognition events take place with different viruses is not clear and the effects of glycosylation…

Correlating Glycoforms of DC‐SIGN with Stability Using a Combination of Enzymatic Digestion and Ion Mobility Mass Spectrometry Open

Hsin‐Yung Yen, Idlir Liko, Joseph Gault, Di Wu, Weston B. Struwe , et al. · 2020

The immune scavenger protein DC‐SIGN interacts with glycosylated proteins and has a putative role in facilitating viral infection. How these recognition events take place with different viruses is not clear and the effects of glycosylation…

Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins Open

Joseph Gault, Idlir Liko, Michael Landreh, Denis Shutin, Jani Reddy Bolla , et al. · 2020

Correlating glycoforms of DC-SIGN with stability using a combination of enzymatic digestion and ion mobility MS Open

Hsin‐Yung Yen, Idlir Liko, Joseph Gault, Di Wu, Weston B. Struwe , et al. · 2020

The immune scavenger protein DC-SIGN interacts with glycosylated proteins and has a putative role in facilitating viral infection. How these recognition events take place with different viruses is not clear and the effects of glycosylation…

Investigating Protein-Lipid Interactions of MthK and Native Bacterial Membrane Lipids Open

Daniel Quetschlich, Mark T. Agasid, Rachel A Davis, Zara A. Sands, Idlir Liko , et al. · 2020

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors Open

Leonhard H. Urner, Idlir Liko, Hsin‐Yung Yen, Kin Kuan Hoi, Jani Reddy Bolla , et al. · 2020

A new azobenzene-based design strategy for detergents in membrane protein research Open

Leonhard H. Urner, Maiko Schulze, Yasmine B. Maier, Waldemar Hoffmann, Stephan Warnke , et al. · 2020

Here, L. H. Urner and co-workers identify a new detergent design strategy for the non-denaturing structural analysis of membrane proteins by studying the gas-phase properties of azobenzene-based oligoglycerol detergents.

A bipartite structural organization defines the SERINC family of HIV-1 restriction factors Open

Valerie E. Pye, Annachiara Rosa, Cinzia Bertelli, Weston B. Struwe, Sarah Maslen , et al. · 2020

Combining Native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins - RAW DATA Open

Joseph Gault, Carol V. Robinson, Idlir Liko, Michael Landreh · 2020

Raw mass spectrometry data. Figures and Extended Data forCombining Native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteinsMain Figures 1,2,3Extended Data 3,4,5Data for supplementary figures available …

PtdIns(4,5)P2 stabilizes active states of GPCRs and enhances selectivity of G-protein coupling Open

Hsin‐Yung Yen, Kin Kuan Hoi, Idlir Liko, George Hedger, Michael R. Horrell , et al. · 2018

Lipid binding attenuates channel closure of the outer membrane protein OmpF Open

Idlir Liko, Matteo T. Degiacomi, Sejeong Lee, Thomas D. Newport, Joseph Gault , et al. · 2018

Significance Outer-membrane porins are often considered as passive conduits of small molecules across lipid bilayers. Using native mass spectrometry experiments we identify a pH-sensitive lipid-binding mechanism of outer membrane porin F, …

Identifying key membrane protein lipid interactions using mass spectrometry Open

Kallol Gupta, Jingwen Li, Idlir Liko, Joseph Gault, Chérine Bechara , et al. · 2018

Ligand binding to a G protein–coupled receptor captured in a mass spectrometer Open

Hsin‐Yung Yen, Jonathan T. S. Hopper, Idlir Liko, Timothy M. Allison, Ya Zhu , et al. · 2017

Drug binding to the P2Y 1 receptor prevents phosphorylation and competes with an endogenous ligand.

Molecular Dynamics Simulations of G-Protein Coupled Receptor Lipid Interactions Open

George Hedger, Hsin‐Yung Yen, Idlir Liko, Carol V. Robinson, Christian Siebold , et al. · 2017

Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters Open

Michael Landreh, Erik G. Marklund, Povilas Uzdavinys, Matteo T. Degiacomi, M. Coincon , et al. · 2017

Mass spectrometry guided structural biology Open

Idlir Liko, Timothy M. Allison, Jonathan T. S. Hopper, Carol V. Robinson · 2016

With the convergence of breakthroughs in structural biology, specifically breaking the resolution barriers in cryo-electron microscopy and with continuing developments in crystallography, novel interfaces with other biophysical methods are…

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