Ievgeniia Zagoriy
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View article: Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography
Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography Open
Centrosomes organize microtubules that are essential for mitotic divisions in animal cells. They consist of centrioles surrounded by pericentriolar material (PCM). Questions related to mechanisms of centriole assembly, PCM organization, an…
View article: Native molecular architectures of centrosomes in<i>C. elegans</i>embryos
Native molecular architectures of centrosomes in<i>C. elegans</i>embryos Open
Centrosomes organize microtubules that are essential for mitotic divisions in animal cells. They consist of centrioles surrounded by Pericentriolar Material (PCM). Questions related to mechanisms of centriole assembly, PCM organization, an…
View article: Genetically encoded multimeric tags for subcellular protein localization in cryo-EM
Genetically encoded multimeric tags for subcellular protein localization in cryo-EM Open
View article: Molecular mechanisms of stress-induced reactivation in mumps virus condensates
Molecular mechanisms of stress-induced reactivation in mumps virus condensates Open
Negative-stranded RNA viruses can establish long-term persistent infection in the form of large intracellular inclusions in the human host and cause chronic diseases. Here, we uncover how cellular stress disrupts the metastable host-virus …
View article: Light and electron microscopy continuum-resolution imaging of 3D cell cultures
Light and electron microscopy continuum-resolution imaging of 3D cell cultures Open
View article: Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM Open
Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is chall…
View article: Cryo-electron tomography of GEM2-labelled Mito-EGFP in HeLa cells
Cryo-electron tomography of GEM2-labelled Mito-EGFP in HeLa cells Open
View article: The Spo13/Meikin pathway confines the onset of gamete differentiation to meiosis II in yeast
The Spo13/Meikin pathway confines the onset of gamete differentiation to meiosis II in yeast Open
View article: Author response: A modular platform for automated cryo-FIB workflows
Author response: A modular platform for automated cryo-FIB workflows Open
View article: Data for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe
Data for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe Open
Repository with input and output files utilized for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe.
View article: Data for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe
Data for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe Open
Repository with input and output files utilized for integrative modeling of the Nuclear Pore Complex from Schizosaccharomyces pombe.
View article: Molecular mechanisms of stress-induced reactivation in mumps virus condensates
Molecular mechanisms of stress-induced reactivation in mumps virus condensates Open
Summary Many viruses can establish long-term persistent infections in the human host and cause chronic diseases. Here we combine cell biology, whole-cell proteomics and cryo-electron tomography to uncover how cellular stress disrupts the h…
View article: Light and electron microscopy continuum-resolution imaging of 3D cell cultures
Light and electron microscopy continuum-resolution imaging of 3D cell cultures Open
Summary 3D cell cultures, in particular organoids, are emerging models to investigate healthy or diseased tissues. Understanding the complex cellular sociology in organoids requires integration of imaging modalities across spatial and temp…
View article: A Modular Platform for Streamlining Automated Cryo-FIB Workflows
A Modular Platform for Streamlining Automated Cryo-FIB Workflows Open
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga + ion beam currents…
View article: Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension
Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tension Open
View article: Nuclear pores constrict upon energy depletion
Nuclear pores constrict upon energy depletion Open
Nuclear pore complexes (NPCs) fuse the inner and outer nuclear membranes and mediate nucleocytoplasmic exchange. They are made of 30 different nucleoporins that form an intricate cylindrical architecture around an aqueous central channel. …
View article: Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies Open
Micromachining by cryo-focused ion beam (FIB) milling coupled to cryo-electron tomography (ET) enables visualization of macromolecules directly inside cells. Yet, spatial control of cell adhesion on electron microscopy (EM) grids remains a…
View article: Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies Open
View article: Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies Open
Spatially-controlled cell adhesion on electron microscopy (EM) supports remains a bottleneck in specimen preparation for cellular cryo-electron tomography. Here, we describe contactless and mask-free photo-micropatterning of EM grids for s…
View article: APC/C-Cdc20 mediates deprotection of centromeric cohesin at meiosis II in yeast
APC/C-Cdc20 mediates deprotection of centromeric cohesin at meiosis II in yeast Open
Cells undergoing meiosis produce haploid gametes through one round of DNA replication followed by 2 rounds of chromosome segregation. This requires that cohesin complexes, which establish sister chromatid cohesion during S phase, are remov…