Ignacio Poblete‐Castro
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View article: Sustainable Open Production of <i>Short</i> – <i>Medium</i> -Chain Length Polyhydroxyalkanoates from Crude Glycerol in Batch and Fed-Batch Bioreactors by <i>Pseudomonas frigusceleri</i> MPC6
Sustainable Open Production of <i>Short</i> – <i>Medium</i> -Chain Length Polyhydroxyalkanoates from Crude Glycerol in Batch and Fed-Batch Bioreactors by <i>Pseudomonas frigusceleri</i> MPC6 Open
Nonsterile microbial synthesis of chemicals offers a promising route to reducing production costs and establishing sustainable processes. Here, we investigated polyhydroxyalkanoate (PHA) production from glycerol by different Pseudomonas st…
View article: Epidemiological surveillance and incidence of respiratory viruses in Chile: Before and after COVID 19
Epidemiological surveillance and incidence of respiratory viruses in Chile: Before and after COVID 19 Open
Objective: During the COVID 19 pandemic, various measures, such as lockdowns and travel restrictions, were implemented to curb the spread of SARS CoV 2, significantly impacting case numbers. These interventions also influenced the incidenc…
View article: <i>Pseudomonas</i> species from Antarctica and other environments host diverse genes encoding structurally conserved polyhydroxyalkanoate synthases linked with different mobile genetic elements
<i>Pseudomonas</i> species from Antarctica and other environments host diverse genes encoding structurally conserved polyhydroxyalkanoate synthases linked with different mobile genetic elements Open
Polyhydroxyalkanoates (PHAs) are industrial microbial biopolymers offering a sustainable and biodegradable alternative to petroleum-based plastics. The PHA polymerization process is primarily mediated by PHA synthases (PhaCs), which determ…
View article: Bulking agent in dry anaerobic digestion as a key factor for the enhancement of biogas production
Bulking agent in dry anaerobic digestion as a key factor for the enhancement of biogas production Open
Dry anaerobic digestion (dry-AD) is an attractive process for solid wastes such as agri-food waste. However, some limitations mainly associated to lack of effective mixing, can hinder the methane production capacity of the systems. Bulking…
View article: Establishing a green biodesulfurization process for iron ore concentrates in stirred tank and leaching column bioreactors using Acidithiobacillus thiooxidans
Establishing a green biodesulfurization process for iron ore concentrates in stirred tank and leaching column bioreactors using Acidithiobacillus thiooxidans Open
The presence of sulfur impurities in complex iron ores represents a significant challenge for the iron mining and steel-making industries as their removal often necessitates the use of hazardous chemicals and energy-intensive processes. He…
View article: Levels of persistent organic pollutants (POPs) in the Antarctic atmosphere over time (1980 to 2021) and estimation of their atmospheric half-lives
Levels of persistent organic pollutants (POPs) in the Antarctic atmosphere over time (1980 to 2021) and estimation of their atmospheric half-lives Open
Persistent organic pollutants (POPs) are synthetic compounds that were intentionally produced in large quantities and have been distributed in the global environment, originating a threat due to their persistence, bioaccumulative potential…
View article: Opportunities and obstacles in microbial synthesis of metal nanoparticles
Opportunities and obstacles in microbial synthesis of metal nanoparticles Open
Metallic nanoparticles (MeNPs) are widely used in many areas such as biomedicine, packaging, cosmetics, colourants, agriculture, antimicrobial agents, cleaning products, as components of electronic devices and nutritional supplements. In a…
View article: Comment on acp-2023-25
Comment on acp-2023-25 Open
Abstract. Persistent organic pollutants (POPs) are synthetic compounds that were intentionally produced in large quantities and have been distributed in the global environment, originating a threat due to their persistence, bioaccumulative…
View article: Levels of persistent organic pollutants (POPs) in the Antarctic atmosphere over time (1980 to 2021) and estimation of their atmospheric half-lives.
Levels of persistent organic pollutants (POPs) in the Antarctic atmosphere over time (1980 to 2021) and estimation of their atmospheric half-lives. Open
Persistent organic pollutants (POPs) are synthetic compounds that were intentionally produced in large quantities and have been distributed in the global environment, originating a threat due to their persistence, bioaccumulative potential…
View article: Discovery of New Phenylacetone Monooxygenase Variants for the Development of Substituted Indigoids through Biocatalysis
Discovery of New Phenylacetone Monooxygenase Variants for the Development of Substituted Indigoids through Biocatalysis Open
Indigoids are natural pigments obtained from plants by ancient cultures. Romans used them mainly as dyes, whereas Asian cultures applied these compounds as treatment agents for several diseases. In the modern era, the chemical industry has…
View article: Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery
Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery Open
Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on petrochemical plastics. One of the most important biodegradable polymers is the family of polyhydroxyalkanoates (PHAs), n…
View article: Biofilm Formation Is Crucial for Efficient Copper Bioleaching From Bornite Under Mesophilic Conditions: Unveiling the Lifestyle and Catalytic Role of Sulfur-Oxidizing Bacteria
Biofilm Formation Is Crucial for Efficient Copper Bioleaching From Bornite Under Mesophilic Conditions: Unveiling the Lifestyle and Catalytic Role of Sulfur-Oxidizing Bacteria Open
Biofilm formation within the process of bioleaching of copper sulfides is a relevant aspect of iron- and sulfur-oxidizing acidophilic microorganisms as it represents their lifestyle in the actual heap/dump mining industry. Here, we used bi…
View article: Fed-Batch mcl- Polyhydroxyalkanoates Production in Pseudomonas putida KT2440 and ΔphaZ Mutant on Biodiesel-Derived Crude Glycerol
Fed-Batch mcl- Polyhydroxyalkanoates Production in Pseudomonas putida KT2440 and ΔphaZ Mutant on Biodiesel-Derived Crude Glycerol Open
Crude glycerol has emerged as a suitable feedstock for the biotechnological production of various industrial chemicals given its high surplus catalyzed by the biodiesel industry. Pseudomonas bacteria metabolize the polyol into several biop…
View article: Photochemistry of P,N-bidentate rhenium(<scp>i</scp>) tricarbonyl complexes: reactive species generation and potential application for antibacterial photodynamic therapy
Photochemistry of P,N-bidentate rhenium(<span>i</span>) tricarbonyl complexes: reactive species generation and potential application for antibacterial photodynamic therapy Open
Three P,N-rhenium tricarbonyl complexes exhibited photoisomerization upon irradiation at 365 nm, and no CO release was detected. The antibacterial activity is attributed to their singlet oxygen generation, following the same decreasing ord…
View article: Editorial: Pathway, Genetic and Process Engineering of Microbes for Biopolymer Synthesis
Editorial: Pathway, Genetic and Process Engineering of Microbes for Biopolymer Synthesis Open
EDITORIAL article Front. Bioeng. Biotechnol., 23 December 2020Sec. Industrial Biotechnology https://doi.org/10.3389/fbioe.2020.618383
View article: Channelling carbon flux through the <i>meta</i> ‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in <i>Pseudomonas putida</i> H
Channelling carbon flux through the <i>meta</i> ‐cleavage route for improved poly(3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in <i>Pseudomonas putida</i> H Open
Engineered P . putida H sets a benchmark in lignin‐based PHA. Balancing the catechol degrading pathways enables improved PHA production. A DO‐stat fed‐batch process permits the efficient conversion of lignin hydrolysates into biomass and b…
View article: Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)
Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates) Open
In the last decade, the development of novel programmable cell lytic systems based on different inducible genetic constructs like the holin–endolysin and lysozyme appears as a promising alternative to circumvent the use of costly enzymes a…
View article: Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response of Bacillus megaterium
Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response of Bacillus megaterium Open
For many years now, Bacillus megaterium serves as a microbial workhorse for the high-level production of recombinant proteins in the g/L-scale. However, efficient and stable production processes require the knowledge of the molecular adapt…
View article: Limited life cycle and cost assessment for the bioconversion of lignin‐derived aromatics into adipic acid
Limited life cycle and cost assessment for the bioconversion of lignin‐derived aromatics into adipic acid Open
Lignin is an abundant and heterogeneous waste byproduct of the cellulosic industry, which has the potential of being transformed into valuable biochemicals via microbial fermentation. In this study, we applied a fast‐pyrolysis process usin…
View article: The Transcription Factor ArcA Modulates Salmonella’s Metabolism in Response to Neutrophil Hypochlorous Acid-Mediated Stress
The Transcription Factor ArcA Modulates Salmonella’s Metabolism in Response to Neutrophil Hypochlorous Acid-Mediated Stress Open
Salmonella Typhimurium, a bacterial pathogen with high metabolic plasticity, can adapt to different environmental conditions; these traits enhance its virulence by enabling bacterial survival. Neutrophils play important roles in the innate…
View article: In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential
In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological Potential Open
We obtained the complete genome sequence of the psychrotolerant extremophile Pseudomonas sp. MPC6, a natural Polyhydroxyalkanoates (PHAs) producing bacterium able to rapidly grow at low temperatures. Genomic and phenotypic analyses allowed…
View article: Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic<i>Pseudomonas</i>strains: from unique phenotypes to novel biopolymers
Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic<i>Pseudomonas</i>strains: from unique phenotypes to novel biopolymers Open
Extreme environments are a unique source of microorganisms encoding metabolic capacities that remain largely unexplored. In this work, we isolated two Antarctic bacterial strains able to produce poly(3-hydroxyalkanoates) (PHAs), which were…
View article: Biochemistry, genetics and biotechnology of glycerol utilization in <i>Pseudomonas</i> species
Biochemistry, genetics and biotechnology of glycerol utilization in <i>Pseudomonas</i> species Open
Summary The use of renewable waste feedstocks is an environment‐friendly choice contributing to the reduction of waste treatment costs and increasing the economic value of industrial by‐products. Glycerol (1,2,3‐propanetriol), a simple pol…