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View article: Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii
Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii Open
Background The β- galactosidase from Paenibacillus wynnii ( β- gal-Pw) is a promising candidate for lactose hydrolysis in milk and dairy products, as it has a higher affinity for the substrate lactose (low K M value) compared to industrial…
View article: A new β-galactosidase from Paenibacillus wynnii with potential for industrial applications
A new β-galactosidase from Paenibacillus wynnii with potential for industrial applications Open
Commercial β-galactosidases exhibit undesirable kinetic properties regarding substrate affinity (Michaelis-Menten constant [KM] for lactose) and product inhibition (inhibitor constant [Ki] for galactose). An in silico screening of gene seq…
View article: The behavior of cathepsin D during milk processing and its contribution to bitterness in a model fresh cheese
The behavior of cathepsin D during milk processing and its contribution to bitterness in a model fresh cheese Open
The bovine endopeptidase cathepsin D was investigated regarding its temperature-dependent inactivation and ability to form bitter peptides within a spiked model fresh cheese. Cathepsin D was found to be more susceptible than other milk end…
View article: Recent advances in the application of microbial diamine oxidases and other histamine-oxidizing enzymes
Recent advances in the application of microbial diamine oxidases and other histamine-oxidizing enzymes Open
The consumption of foods fraught with histamine can lead to various allergy-like symptoms if the histamine is not sufficiently degraded in the human body. The degradation occurs primarily in the small intestine, naturally catalyzed by the …
View article: Toward Oral Supplementation of Diamine Oxidase for the Treatment of Histamine Intolerance
Toward Oral Supplementation of Diamine Oxidase for the Treatment of Histamine Intolerance Open
A new diamine oxidase (DAO-1) was discovered recently in the yeast Yarrowia lipolytica PO1f and investigated for its histamine degradation capability under simulated intestinal conditions. DAO-1 was formulated together with catalase as a s…
View article: Orotic acid production by <i>Yarrowia lipolytica</i> under conditions of limited pyrimidine
Orotic acid production by <i>Yarrowia lipolytica</i> under conditions of limited pyrimidine Open
Orotic acid (OA) is an intermediate of the pyrimidine biosynthesis with high industrial relevance due to its use as precursor for production of biochemical pyrimidines or its use as carrier molecule in drug formulations. It can be produced…
View article: Evaluation of porcine diamine oxidase for the conversion of histamine in food‐relevant amounts
Evaluation of porcine diamine oxidase for the conversion of histamine in food‐relevant amounts Open
Histamine exists in a multitude of foods and displays an emerging role within food intolerances. Our aim was to identify the activity of porcine diamine oxidase (DAO) required for the in vitro degradation of histamine amounts that are foun…
View article: Determination of the Direct Activity of the Maltogenic Amylase from Geobacillus stearothermophilus in White Bread
Determination of the Direct Activity of the Maltogenic Amylase from Geobacillus stearothermophilus in White Bread Open
An assay-based method was developed to determine the residual activity of the maltogenic amylase from Geobacillus stearothermophilus in white bread. It was found that the important step for amylase extraction from the bread matrix was the …
View article: Functional and structural studies of a C-terminally extended YidC
Functional and structural studies of a C-terminally extended YidC Open
Members of the YidC/Oxa1/Alb3 protein family catalyze the insertion of integral membrane proteins into the lipid bilayer of the bacterial plasma membrane (YidC), the inner mitochondrial membrane (Oxa1), and the chloroplast thylakoid membra…