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View article: β-Caryophyllene Inhibits Monoacylglycerol Lipase Activity and Increases 2-Arachidonoyl Glycerol Levels In Vivo: A New Mechanism of Endocannabinoid-Mediated Analgesia?
β-Caryophyllene Inhibits Monoacylglycerol Lipase Activity and Increases 2-Arachidonoyl Glycerol Levels In Vivo: A New Mechanism of Endocannabinoid-Mediated Analgesia? Open
The mechanisms of β-caryophyllene (BCP)-induced analgesia are not well studied. Here, we tested the efficacy of BCP in an acute postsurgical pain model and evaluated its effect on the endocannabinoid system. Rats were treated with v…
View article: Evaluation of the National Competence Based Catalogue of Learning Objectives (NKLM 2.0) for undergraduate medical education at the Medical School Hannover
Evaluation of the National Competence Based Catalogue of Learning Objectives (NKLM 2.0) for undergraduate medical education at the Medical School Hannover Open
Background: The National Competence-Based Catalogue of Learning Objectives for Undergraduate Medical Education (NKLM) serves as the foundation for curricular development in undergraduate medical education in Germany. A new version of the N…
View article: Clostridium botulinum C3bot mediated effects on cytokine-induced psoriasis-like phenotype in full-thickness skin model
Clostridium botulinum C3bot mediated effects on cytokine-induced psoriasis-like phenotype in full-thickness skin model Open
Clostridium botulinum C3 exoenzyme (C3bot) exclusively inhibits RhoA, B and C by ADP-ribosylation and is therefore used as a cell-permeable tool for investigating the cellular role of these Rho-GTPases. Rho-GTPases represent a molecular sw…
View article: Clostridium botulinum C3bot mediated effects on cytokine-induced psoriasis-like phenotype in full-thickness skin model
Clostridium botulinum C3bot mediated effects on cytokine-induced psoriasis-like phenotype in full-thickness skin model Open
Clostridium botulinum C3 exoenyzme (C3bot) exclusively inhibits RhoA, B and C by ADP-ribosylation and is therefore used as a cell permeable tool for investigating the cellular role of these Rho-GTPases. Rho-GTPases represent a molecular sw…
View article: Corrigendum: Potentiation of brain-derived neurotrophic factor-induced protection of spiral ganglion neurons by C3 exoenzyme/Rho inhibitor
Corrigendum: Potentiation of brain-derived neurotrophic factor-induced protection of spiral ganglion neurons by C3 exoenzyme/Rho inhibitor Open
[This corrects the article DOI: 10.3389/fncel.2021.602897.].
View article: Enhancement of Phosphorylation and Transport Activity of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter 3 by C3bot and a 26mer C3bot Peptide
Enhancement of Phosphorylation and Transport Activity of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter 3 by C3bot and a 26mer C3bot Peptide Open
In primary murine hippocampal neurons we investigated the regulation of EAAT3-mediated glutamate transport by the Clostridium botulinum C3 transferase C3bot and a 26mer peptide derived from full length protein. Incubation with either enzym…
View article: Editorial: The Deadly Secrets of C. Difficile—Insights Into Host-Pathogen Interaction, Volume II
Editorial: The Deadly Secrets of C. Difficile—Insights Into Host-Pathogen Interaction, Volume II Open
EDITORIAL article Front. Microbiol., 06 April 2022Sec. Infectious Agents and Disease https://doi.org/10.3389/fmicb.2022.896979
View article: Editorial: The Deadly Secrets of C. difficile—Insights Into Host-Pathogen Interaction
Editorial: The Deadly Secrets of C. difficile—Insights Into Host-Pathogen Interaction Open
EDITORIAL article Front. Microbiol., 06 April 2022Sec. Infectious Agents and Disease https://doi.org/10.3389/fmicb.2022.897612
View article: β-Caryophyllene inhibits monoacylglycerol lipase activity and increases 2-AG levels: a new mechanism of endocannabinoid-mediated analgesia?
β-Caryophyllene inhibits monoacylglycerol lipase activity and increases 2-AG levels: a new mechanism of endocannabinoid-mediated analgesia? Open
Introduction. β-Caryophyllene (BCP) has been shown to be an effective anti-inflammatory agent in chronic and inflammatory pain models. Since limited data are available for BCP in acute pain, we tested efficacy of BCP in an acute post-surgi…
View article: Potentiation of Brain-Derived Neurotrophic Factor-Induced Protection of Spiral Ganglion Neurons by C3 Exoenzyme/Rho Inhibitor
Potentiation of Brain-Derived Neurotrophic Factor-Induced Protection of Spiral Ganglion Neurons by C3 Exoenzyme/Rho Inhibitor Open
Preservation of the excitability of spiral ganglion neurons (SGN) may contribute to an improved speech perception after cochlear implantation. Thus, the application of exogenous neurotrophic factors such as the neurotrophin brain-derived n…
View article: Classroom teaching with simulated patients during COVID-19: the communication skills course in the second year of the model medical curriculum HannibaL
Classroom teaching with simulated patients during COVID-19: the communication skills course in the second year of the model medical curriculum HannibaL Open
Objective: In the spring of 2020 in response to the COVID-19 pandemic, the question arose at Hannover Medical School as to how simulated patients (SP) could still be utilized in the communication course that is part of the module “Diagnost…
View article: The Higher Sensitivity of GABAergic Compared to Glutamatergic Neurons to Growth-Promoting C3bot Treatment Is Mediated by Vimentin
The Higher Sensitivity of GABAergic Compared to Glutamatergic Neurons to Growth-Promoting C3bot Treatment Is Mediated by Vimentin Open
The current study investigates the neurotrophic effects of Clostridium botulinum C3 transferase (C3bot) on highly purified, glia-free, GABAergic, and glutamatergic neurons. Incubation with nanomolar concentrations of C3bot promotes dendrit…
View article: HannibaL – the model curriculum at Hannover Medical School: targets, implementation and experiences
HannibaL – the model curriculum at Hannover Medical School: targets, implementation and experiences Open
Aim: The model curriculum known as HannibaL is an integrated, professionally-based adaptive curriculum that began at the Hannover Medical School (MHH) during the 2005/06 academic year. HannibaL turns medical students into competent physici…
View article: Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018
Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018 Open
Clostridioides difficile toxin A (TcdA) and Toxin B (TcdB) trigger inflammasome activation with caspase-1 activation in cultured cells, which in turn induce the release of IL-6, IFN-γ, and IL-8. Release of these proinflammatory resp…
View article: Quantitative Phosphoproteome Analysis of Clostridioides difficile Toxin B Treated Human Epithelial Cells
Quantitative Phosphoproteome Analysis of Clostridioides difficile Toxin B Treated Human Epithelial Cells Open
The large clostridial glucosylating toxin B (TcdB) is a major virulence factor of the nosocomial pathogen Clostridioides difficile. TcdB inhibits small GTPases by glucosylation leading to impaired downstream signaling. TcdB also pos…
View article: Evidence for an Adaptation of a Phage-Derived Holin/Endolysin System to Toxin Transport in Clostridioides difficile
Evidence for an Adaptation of a Phage-Derived Holin/Endolysin System to Toxin Transport in Clostridioides difficile Open
The pathogenicity locus (PaLoc) of Clostridioides difficile usually comprises five genes (tcdR, tcdB, tcdE, tcdA, tcdC). While the proteins TcdA and TcdB represent the main toxins of this pathogen, TcdR and TcdC are involved in the regulat…
View article: The impact of the program structure at Hannover Medical School on academic success in medical studies
The impact of the program structure at Hannover Medical School on academic success in medical studies Open
Aim: The classical course structure for medicine in Germany is separated by three sections of the medical state examination. This structure is generally regarded as sensible and unchangeable. Because the special program structure at Hannov…
View article: Rho-GTPase und p38 vermittelte Neuroprotektion in Spiralganglienzellen
Rho-GTPase und p38 vermittelte Neuroprotektion in Spiralganglienzellen Open
Die Verabreichung von neuroprotektiven und neuroregenerativen Wirkstoffen ist im Bereich der Cochlea Implantat (CI) Forschung von immenser Bedeutung. Besonders im Hinblick auf die Entwicklung neuartiger biologischer Therapien ist die Ident…
View article: Rho-GTPase and p38 mediated neuroprotection in spiral ganglion cells
Rho-GTPase and p38 mediated neuroprotection in spiral ganglion cells Open
The administration of neuroprotective and neuroregenerative factors is of great importance in the field of cochlear implant (CI) research. Particularly with regard to the development of novel biological therapies, the identification of sui…
View article: Anti-proliferative Effect of C3 Exoenzyme in Fibroblasts is Mediated by c-Jun Phosphorylation
Anti-proliferative Effect of C3 Exoenzyme in Fibroblasts is Mediated by c-Jun Phosphorylation Open
Supplemental Figure S1: C3-mediated ADP-ribosylation of Rho in p38-/-p38 and p38 -/-EV MEFs.Both cell lines were incubated with 500 nM C3 or 500 nM C3-E174Q for indicated time points.Cells were lysed and applied to Western blot analyses fo…
View article: Anti-proliferative Effect of C3 Exoenzyme in Fibroblasts is Mediated by c-Jun Phosphorylation
Anti-proliferative Effect of C3 Exoenzyme in Fibroblasts is Mediated by c-Jun Phosphorylation Open
The ADP-ribosyltransferase C3 exoenzyme from C. botulinum selectively inactivates Rho and is therefore often used as an inhibitor for investigations on Rho signaling. Previous studies of our group revealed that C3 inhibited cell pro…
View article: C3 exoenzyme impairs cell proliferation and apoptosis by altering the activity of transcription factors
C3 exoenzyme impairs cell proliferation and apoptosis by altering the activity of transcription factors Open
View article: Metal Ion Activation of Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B
Metal Ion Activation of Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B Open
Lethal Toxin from Clostridium sordellii (TcsL) and Toxin B from Clostridium difficile (TcdB) belong to the family of the “Large clostridial glycosylating toxins.” These toxins mono-O-glucosylate low molecular weight GTPases of the Rho and …
View article: Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody
Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody Open
Clostridium botulinum exoenzyme C3 is the prototype of C3-like ADP-ribosyltransferases that modify the GTPases RhoA, B, and C. C3 catalyzes the transfer of an ADP-ribose moiety from the co-substrate nicotinamide adenine dinucleotide (NAD) …
View article: Cell Entry of C3 Exoenzyme from Clostridium botulinum
Cell Entry of C3 Exoenzyme from Clostridium botulinum Open
View article: Cellular uptake of TcdA, TcdA<sup>1–1874</sup> and TcdA<sup>1875–2710</sup>.
Cellular uptake of TcdA, TcdA<sup>1–1874</sup> and TcdA<sup>1875–2710</sup>. Open
A) Efficiencies of TcdA and TcdA1–1874 uptake into HT29 cells were indirectly determined by monitoring lysosomal toxin degradation following endosomal acidification. After binding at 4°C, endocytosis of toxin was allowed by temp…
View article: Vimentin is present at the cell surface of HT22 cells and J774A.1 macrophages.
Vimentin is present at the cell surface of HT22 cells and J774A.1 macrophages. Open
A) Intact HT22 cells were biotinylated for 1 h at 4°C. Whole cell lysates, cytosolic and particulate fractions were prepared. In addition, cell surface biotinylated proteins were enriched by precipitation with NeutrAvidin beads. The fracti…
View article: Make Me Laugh: Recommending Humoristic Content on the WWW
Make Me Laugh: Recommending Humoristic Content on the WWW Open
Humoristic content is an inherent part of the World Wide Web and increasingly consumed for microentertainment.However, humor is often highly individual and depends on background knowledge and context.This paper presents an approach to reco…
View article: Uptake of Clostridium botulinum C3 Exoenzyme into Intact HT22 and J774A.1 Cells
Uptake of Clostridium botulinum C3 Exoenzyme into Intact HT22 and J774A.1 Cells Open
The Clostridium botulinum C3 exoenzyme selectively ADP-ribosylates low molecular weight GTP-binding proteins RhoA, B and C. This covalent modification inhibits Rho signaling activity, resulting in distinct actin cytoskeleton changes. Altho…