Ioanna A. Thanasi
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View article: Formation of mono- and dual-labelled antibody fragment conjugates <i>via</i> reversible site-selective disulfide modification and proximity induced lysine reactivity
Formation of mono- and dual-labelled antibody fragment conjugates <i>via</i> reversible site-selective disulfide modification and proximity induced lysine reactivity Open
Exploiting quantitative and reversible site-selective disulfide modification as a means for selective lysine functionalisation on clinically relevant antibody fragments.
View article: Site-directed conjugation of single-stranded DNA to affinity proteins: quantifying the importance of conjugation strategy
Site-directed conjugation of single-stranded DNA to affinity proteins: quantifying the importance of conjugation strategy Open
Site-selective conjugation of ssDNA to affinity proteins grants improved target binding and lower non-specific binding.
View article: Chemo- and regio-selective differential modification of native cysteines on an antibody <i>via</i> the use of dehydroalanine forming reagents
Chemo- and regio-selective differential modification of native cysteines on an antibody <i>via</i> the use of dehydroalanine forming reagents Open
Use of dehydroalanine-forming reagents to enable the first example of differentially modifying the native solvent accessible cysteines on an antibody.
View article: CCDC 2296018: Experimental Crystal Structure Determination
CCDC 2296018: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: Site-directed Conjugation of Single-Stranded DNA to Affinity Proteins: Quantifying the Importance of Conjugation Strategy
Site-directed Conjugation of Single-Stranded DNA to Affinity Proteins: Quantifying the Importance of Conjugation Strategy Open
Affinity protein–oligonucleotide conjugates are increasingly being explored as diagnostic and therapeutic tools. Despite growing interest, these probes are typically constructed using outdated, non-selective chemistries, and little has bee…
View article: Site-directed Conjugation of Single-Stranded DNA to Affinity Proteins: Quantifying the Importance of Conjugation Strategy
Site-directed Conjugation of Single-Stranded DNA to Affinity Proteins: Quantifying the Importance of Conjugation Strategy Open
Affinity protein–oligonucleotide conjugates are increasingly being explored as diagnostic and therapeutic tools. Despite growing interest, these probes are typically constructed using outdated, non-selective chemistries, and little has bee…
View article: Modular Synthesis of Semiconducting Graft Copolymers to Achieve “Clickable” Fluorescent Nanoparticles with Long Circulation and Specific Cancer Targeting
Modular Synthesis of Semiconducting Graft Copolymers to Achieve “Clickable” Fluorescent Nanoparticles with Long Circulation and Specific Cancer Targeting Open
Semiconducting polymer nanoparticles (SPNs) are explored for applications in cancer theranostics because of their high absorption coefficients, photostability, and biocompatibility. However, SPNs are susceptible to aggregation and protein …
View article: Modular Chemical Construction of IgG-like Mono- and Bispecific Synthetic Antibodies (SynAbs)
Modular Chemical Construction of IgG-like Mono- and Bispecific Synthetic Antibodies (SynAbs) Open
In recent years there has been rising interest in the field of protein-protein conjugation, especially related to bispecific antibodies (bsAbs) and their therapeutic applications. These constructs contain two paratopes capable of binding t…
View article: Use of pyridazinediones for tuneable and reversible covalent cysteine modification applied to peptides, proteins and hydrogels
Use of pyridazinediones for tuneable and reversible covalent cysteine modification applied to peptides, proteins and hydrogels Open
Substituted pyridazinediones (PDs) enable reversible and tuneable cysteine modification with exemplification on peptides, proteins and hydrogels.
View article: Modular chemical construction of IgG-like mono- and bispecific synthetic antibodies (SynAbs)
Modular chemical construction of IgG-like mono- and bispecific synthetic antibodies (SynAbs) Open
In recent years there has been rising interest in the field of protein–protein conjugation, especially related to bispecific antibodies (bsAbs) and their therapeutic applications. These constructs contain two paratopes capable of binding t…
View article: Dual reactivity disulfide bridging reagents; enabling new approaches to antibody fragment bioconjugation
Dual reactivity disulfide bridging reagents; enabling new approaches to antibody fragment bioconjugation Open
Here we describe the first examples of a new class of disulfide bridging reagents, designed to insert a ‘stable-labile’ linkage; which can then be exploited to generate dual functional antibody fragment conjugates.
View article: A novel thiol-labile cysteine protecting group for peptide synthesis based on a pyridazinedione (PD) scaffold
A novel thiol-labile cysteine protecting group for peptide synthesis based on a pyridazinedione (PD) scaffold Open
Herein we establish the compatibility of pyridazinediones (PDs) as thiol-labile cysteine protecting groups in solid phase peptide synthesis (SPPS).