Iran A. N. Silva
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View article: Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos
Label-Free High-Resolution Photothermal Optical Infrared Spectroscopy for Spatiotemporal Chemical Analysis in Fresh, Hydrated Living Tissues and Embryos Open
Label-free chemical imaging of living and functioning systems is the holy grail of biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many …
View article: Deep learning for rapid and reproducible histology scoring of lung injury in a porcine model
Deep learning for rapid and reproducible histology scoring of lung injury in a porcine model Open
Acute respiratory distress syndrome (ARDS) is a life-threatening condition with mortality rates between 30-50%. Although in vitro models replicate some aspects of ARDS, small and large animal models remain the primary research tools due to…
View article: Formalin-free fixation and xylene-free tissue processing preserves cell-hydrogel interactions for histological evaluation of 3D calcium alginate tissue engineered constructs
Formalin-free fixation and xylene-free tissue processing preserves cell-hydrogel interactions for histological evaluation of 3D calcium alginate tissue engineered constructs Open
Histological evaluation of tissue-engineered products, including hydrogels for cellular encapsulation, is a critical and invaluable tool for assessing the product across multiple stages of its lifecycle from manufacture to implantation. Ho…
View article: Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems
Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems Open
Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sampl…
View article: Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations
Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations Open
The respiratory epithelium consists of multiple, functionally distinct cell types and is maintained by regionally specific progenitor populations that repair the epithelium following injury. Several in vitro methods exist for studying lung…
View article: Reduction of primary graft dysfunction using cytokine adsorption during organ preservation and after lung transplantation
Reduction of primary graft dysfunction using cytokine adsorption during organ preservation and after lung transplantation Open
Despite improvements, lung transplantation remains hampered by both a scarcity of donor organs and by mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits donor lungs utilization, we …
View article: Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations
Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations Open
Summary The respiratory epithelium consists of multiple, functionally distinct cell-types and is maintained by regionally-specific progenitor populations which repair the epithelium following injury. Several in vitro methods exist for stud…
View article: A Semi-quantitative Scoring System for Green Histopathological Evaluation of Large Animal Models of Acute Lung Injury
A Semi-quantitative Scoring System for Green Histopathological Evaluation of Large Animal Models of Acute Lung Injury Open
Acute respiratory distress syndrome (ARDS) is a life-threatening, high mortality pulmonary condition characterized by acute lung injury (ALI) resulting in diffuse alveolar damage. Despite progress regarding the understanding of ARDS pathop…
View article: Lung transplant after 6 months on ECMO support for SARS-CoV-2-induced ARDS complicated by severe antibody-mediated rejection
Lung transplant after 6 months on ECMO support for SARS-CoV-2-induced ARDS complicated by severe antibody-mediated rejection Open
There have been a few reports of successful lung transplantation (LTx) in patients with SARS-CoV-2-induced acute respiratory distress syndrome (ARDS); however, all reports were with rather short follow-up. Here we present a 62-year-old man…
View article: Monitoring lung injury with particle flow rate in LPS‐ and COVID‐19‐induced ARDS
Monitoring lung injury with particle flow rate in LPS‐ and COVID‐19‐induced ARDS Open
In severe acute respiratory distress syndrome (ARDS), extracorporeal membrane oxygenation (ECMO) is a life-prolonging treatment, especially among COVID-19 patients. Evaluation of lung injury progression is challenging with current techniqu…
View article: Reduction of primary graft dysfunction using cytokine filtration following acute respiratory distress syndrome in lung transplantation
Reduction of primary graft dysfunction using cytokine filtration following acute respiratory distress syndrome in lung transplantation Open
Despite improvements, lung transplantation (LTx) remains hampered by both a scarcity of donor organs and by mortality following primary graft dysfunction (PGD). Since acute respiratory distress syndrome (ARDS) limits donor lungs utilizatio…
View article: Extracellular‐Matrix‐Reinforced Bioinks for 3D Bioprinting Human Tissue
Extracellular‐Matrix‐Reinforced Bioinks for 3D Bioprinting Human Tissue Open
Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a …
View article: Increased particle flow rate from airways precedes clinical signs of ARDS in a porcine model of LPS-induced acute lung injury
Increased particle flow rate from airways precedes clinical signs of ARDS in a porcine model of LPS-induced acute lung injury Open
Acute respiratory distress syndrome (ARDS) is a common cause of death in the intensive care unit, with mortality rates of ~30–40%. To reduce invasive diagnostics such as bronchoalveolar lavage and time-consuming in-hospital transports for …