Irit Huber
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View article: XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector
XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector Open
Ectopic expression of proteins in human pluripotent stem cells (hPSCs) is highly desirable as a research tool and important for clinical translation. However, genetically engineering hPSCs for long-term overexpression of proteins remains i…
View article: Utilizing Human-Induced Pluripotent Stem Cells to Study Cardiac Electroporation Pulsed-Field Ablation
Utilizing Human-Induced Pluripotent Stem Cells to Study Cardiac Electroporation Pulsed-Field Ablation Open
BACKGROUND: Electroporation is a promising nonthermal ablation method for cardiac arrhythmia treatment. Although initial clinical studies found electroporation pulsed-field ablation (PFA) both safe and efficacious, there are significant kn…
View article: Unraveling the pathogenesis of novel ANKRD1 missense mutation causing familial dilated cardiomyopathy using human-induced pluripotent stem cells derived cardiomyocytes
Unraveling the pathogenesis of novel ANKRD1 missense mutation causing familial dilated cardiomyopathy using human-induced pluripotent stem cells derived cardiomyocytes Open
Introduction Familial dilated cardiomyopathy(f-DCM) can be caused by mutations in 30 different genes encoding sarcomeric, cytoskeletal, mechanotransducers and transcription factors. One of the genes ANKRD1 encode Cardiac ankyrin repeat pro…
View article: Utilizing human induced pluripotent stem cells to study atrial arrhythmias in the short QT syndrome
Utilizing human induced pluripotent stem cells to study atrial arrhythmias in the short QT syndrome Open
View article: BS-454195-3 GENERATION OF A NOVEL IN-VITRO MODEL OF THE INFARCT BORDER ZONE TO STUDY HUMAN VENTRICULAR ARRHYTHMIAS
BS-454195-3 GENERATION OF A NOVEL IN-VITRO MODEL OF THE INFARCT BORDER ZONE TO STUDY HUMAN VENTRICULAR ARRHYTHMIAS Open
View article: PO-455617-4 GENERATION OF A NOVEL IN-VITRO MODEL OF THE INFARCT BORDER ZONE TO STUDY HUMAN VENTRICULAR ARRHYTHMIAS
PO-455617-4 GENERATION OF A NOVEL IN-VITRO MODEL OF THE INFARCT BORDER ZONE TO STUDY HUMAN VENTRICULAR ARRHYTHMIAS Open
View article: shRNAs Targeting a Common KCNQ1 Variant Could Alleviate Long-QT1 Disease Severity by Inhibiting a Mutant Allele
shRNAs Targeting a Common KCNQ1 Variant Could Alleviate Long-QT1 Disease Severity by Inhibiting a Mutant Allele Open
Long-QT syndrome type 1 (LQT1) is caused by mutations in KCNQ1. Patients heterozygous for such a mutation co-assemble both mutant and wild-type KCNQ1-encoded subunits into tetrameric Kv7.1 potassium channels. Here, we investigated whether …
View article: Characterization of the mechanism by which a nonsense variant in <i>RYR2</i> leads to disordered calcium handling
Characterization of the mechanism by which a nonsense variant in <i>RYR2</i> leads to disordered calcium handling Open
Heterozygous missense variants of the cardiac ryanodine receptor gene (RYR2) cause catecholaminergic polymorphic ventricular tachycardia (CPVT). These missense variants of RYR2 result in a gain of function of the ryanodine receptors, chara…
View article: Optogenetic Control of Human Induced Pluripotent Stem Cell‐Derived Cardiac Tissue Models
Optogenetic Control of Human Induced Pluripotent Stem Cell‐Derived Cardiac Tissue Models Open
Background Optogenetics, using light‐sensitive proteins, emerged as a unique experimental paradigm to modulate cardiac excitability. We aimed to develop high‐resolution optogenetic approaches to modulate electrical activity in 2‐ and 3‐dim…
View article: Light-controllable recellularized ventricular and atrial tissues
Light-controllable recellularized ventricular and atrial tissues Open
Introduction Construction of three-dimensional ventricular and atrial patches using decellularized extracellular matrix could allow the creation of “organ-like” structures for disease modeling, drug testing and regenerative medicine applic…
View article: Study and modulation of cardiac electroporation with a novel model utilizing human induced pluripotent stem cells
Study and modulation of cardiac electroporation with a novel model utilizing human induced pluripotent stem cells Open
Background Cardiac electroporation is a promising novel non-thermal ablation method, gaining significant interest with recent first-in-man data suggesting effective cardiac lesion generation with no collateral damage. Nevertheless, signifi…
View article: Optogenetic modulation of cardiac action potential properties may prevent arrhythmogenesis in short and long QT syndromes
Optogenetic modulation of cardiac action potential properties may prevent arrhythmogenesis in short and long QT syndromes Open
Abnormal action potential (AP) properties, as occurs in long or short QT syndromes (LQTS and SQTS, respectively), can cause life-threatening arrhythmias. Optogenetics strategies, utilizing light-sensitive proteins, have emerged as experime…
View article: Characterisation of the mechanism by which a nonsense variant in <i>RYR2</i> results in ventricular arrhythmia
Characterisation of the mechanism by which a nonsense variant in <i>RYR2</i> results in ventricular arrhythmia Open
Background Heterozygous variants in the cardiac ryanodine receptor gene ( RYR2 ) cause catecholaminergic polymorphic ventricular tachycardia (CPVT). Most pathogenic RYR2 variants are missense variants which result in a gain of function, ca…
View article: Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues
Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues Open
View article: Modeling Reentry in the Short QT Syndrome With Human-Induced Pluripotent Stem Cell–Derived Cardiac Cell Sheets
Modeling Reentry in the Short QT Syndrome With Human-Induced Pluripotent Stem Cell–Derived Cardiac Cell Sheets Open
View article: Human Induced Pluripotent Stem Cell-Derived Cardiac Cell Sheets Expressing Genetically Encoded Voltage Indicator for Pharmacological and Arrhythmia Studies
Human Induced Pluripotent Stem Cell-Derived Cardiac Cell Sheets Expressing Genetically Encoded Voltage Indicator for Pharmacological and Arrhythmia Studies Open
Fulfilling the potential of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for studying conduction and arrhythmogenesis requires development of multicellular models and methods for long-term repeated tissue phenotyping.…
View article: Patient-Specific Drug Screening Using a Human Induced Pluripotent Stem Cell Model of Catecholaminergic Polymorphic Ventricular Tachycardia Type 2
Patient-Specific Drug Screening Using a Human Induced Pluripotent Stem Cell Model of Catecholaminergic Polymorphic Ventricular Tachycardia Type 2 Open
Background— Catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) results from autosomal recessive CASQ2 mutations, causing abnormal Ca 2+ -handling and malignant ventricular arrhythmias. We aimed to establish a patient-spec…
View article: Monitoring Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes with Genetically Encoded Calcium and Voltage Fluorescent Reporters
Monitoring Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes with Genetically Encoded Calcium and Voltage Fluorescent Reporters Open