Explanipedia

Rapid adaptation of cyanobacteria to environmental perturbations is achieved through structural remodeling of the proteome Open

Snigdha Sarkar, Elise M. Van Fossen, Xiaolu Li, Tong Zhang, Song Feng , et al. · 2025

Dynamic environments require cyanobacteria to rapidly respond to fluctuating light conditions on timescales faster than transcription-translation processes allow, which is possible through immediate regulation of protein function via molec…

Sorted-cell proteomics reveals an AT1-associated epithelial cornification phenotype and suggests endothelial redox imbalance in human bronchopulmonary dysplasia Open

Mereena George Ushakumary, William Chrisler, Gautam Bandyopadhyay, Heidie Huyck, Brittney Gorman , et al. · 2025

The study is the first to perform proteomics on sorted pulmonary epithelial and endothelial populations from bronchopulmonary dysplasia (BPD) and age-matched control human donors. We identified an increase in cornification-associated prote…

Human Coronavirus-229E Hijacks Key Host-Cell RNA-Processing Complexes for Replication Open

Snigdha Sarkar, Song Feng, Hugh Mitchell, Madelyn Berger, Tong Zhang , et al. · 2025

The recent rise in zoonotic coronavirus outbreaks underscores the urgency to understand virus-host interactions to develop potent antiviral therapeutics. Systems biology approaches, particularly proteomics, have been invaluable in providin…

Sorted-Cell Proteomics Reveals an AT1-Associated Epithelial Cornification Phenotype and Suggests Endothelial Redox Imbalance in Human Bronchopulmonary Dysplasia Open

Mereena George Ushakumary, William Chrisler, Gautam Bandyopadhyay, Heidie Huyck, Brittney Gorman , et al. · 2025

Bronchopulmonary dysplasia (BPD) is a neonatal lung disease characterized by inflammation and scarring leading to long-term tissue damage. Previous whole tissue proteomics identified BPD-specific proteome changes and cell type shifts. Litt…

Structural, functional, and regulatory evaluation of a cysteine post-translationally modified Gcn5-related N-acetyltransferase Open

Patricia Uychoco, K.A. Majorek, Ashley N. Ives, Van Thi Bich Le, P. Silva , et al. · 2025

Polyamines within the cell are tightly regulated by spermidine/spermine N-acetyltransferase (SSAT) enzymes. While several SSATs have been investigated in different bacterial species, there is still a significant gap in knowledge about whic…

APOA2 increases cholesterol efflux capacity to plasma HDL by displacing the C-terminus of resident APOA1 Open

Snigdha Sarkar, Jamie Morris, Youngki You, Hannah Sexmith, Scott E Street , et al. · 2024

Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue Microenvironment Open

Marija Veličković, Thomas Fillmore, Isaac Attah, Camilo Posso, James C. Pino , et al. · 2024

There is increasing interest in developing in-depth proteomic approaches for mapping tissue heterogeneity in a cell-type-specific manner to better understand and predict the function of complex biological systems such as human organs. Exis…

Ion Mobility Separations Using Cocentric Architecture Open

Pearl Kwantwi-Barima, Adam Hollerbach, Isaac Attah, Randolph V. Norheim, Yehia Ibrahim · 2024

Ion mobility separations, especially using drift tube ion mobility spectrometers, are usually performed in linear channels, which can have a large footprint when extended to achieve higher resolving powers. In this work, we explored the pe…

O-GlcNAc transferase regulates collagen deposition and fibrosis resolution in idiopathic pulmonary fibrosis Open

Shia Vang, E. Scott Helton, Yiming Guo, Bailey Burpee, Elex Rose , et al. · 2024

Background Idiopathic pulmonary fibrosis (IPF) is a chronic pulmonary disease that is characterized by an excessive accumulation of extracellular matrix (ECM) proteins (e.g. collagens) in the parenchyma, which ultimately leads to respirato…

Signatures of cysteine oxidation on muscle structural and contractile proteins are associated with physical performance and muscle function in older adults: Study of Muscle, Mobility and Aging (SOMMA) Open

Nicholas Day, Shane S. Kelly, Li‐Yung Lui, Tyler A. Mansfield, Matthew Gaffrey , et al. · 2024

Oxidative stress is considered a contributor to declining muscle function and mobility during aging; however, the underlying molecular mechanisms remain poorly described. We hypothesized that greater levels of cysteine (Cys) oxidation on m…

Accumulation of Large Ion Populations with High Ion Densities and Effects Due to Space Charge in Traveling Wave-Based Structures for Lossless Ion Manipulations (SLIM) IMS-MS Open

Pearl Kwantwi-Barima, Sandilya Garimella, Isaac Attah, Xueyun Zheng, Yehia Ibrahim , et al. · 2024

The accumulation of very large ion populations in traveling wave (TW)-based Structures for Lossless ion Manipulations (SLIM) has been studied to better understand aspects of "in-SLIM" ion accumulation, and particularly its use in conjuncti…