Isabelle Becher
YOU?
Author Swipe
View article: High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria
High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria Open
Systematic mapping of protein–ligand interactions is essential for understanding biological processes and drug mechanisms. Peptide-centric local stability assay (PELSA) is a powerful tool for detecting these interactions and identifying po…
View article: High-throughput peptide-centric local stability assay extends protein-ligand identification to membrane proteins, tissues, and bacteria
High-throughput peptide-centric local stability assay extends protein-ligand identification to membrane proteins, tissues, and bacteria Open
Systematic mapping of protein-ligand interactions is essential for understanding biological processes and drug mechanisms. Peptide-centric local stability assay (PELSA) is a powerful tool for detecting these interactions and localizing pot…
View article: Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections
Highly potent quinoxalinediones inhibit α-hemolysin and ameliorate Staphylococcus aureus lung infections Open
Hospital-acquired pneumonia caused by Staphylococcus aureus is associated with patient morbidity and mortality, despite adequate antibiotic therapy. This illustrates the need for treatments beyond antibiotics. The pore-forming heptameric t…
View article: Jumbo phage killer immune system targets early infection of nucleus-forming phages
Jumbo phage killer immune system targets early infection of nucleus-forming phages Open
View article: Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco)
Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco) Open
View article: Pervasive phosphorylation by phage T7 kinase disarms bacterial defenses
Pervasive phosphorylation by phage T7 kinase disarms bacterial defenses Open
Bacteria and bacteriophages are in a constant arms race to develop bacterial defense and phage counter-defense systems. Currently known phage counter-defense systems are specific to (the activity of) the targeted bacterial defense system. …
View article: Dextromethorphan inhibits collagen and collagen-like cargo secretion to ameliorate lung fibrosis
Dextromethorphan inhibits collagen and collagen-like cargo secretion to ameliorate lung fibrosis Open
Excessive deposition of fibrillar collagen in the interstitial extracellular matrix (ECM) of human lung tissue causes fibrosis, which can ultimately lead to organ failure. Despite our understanding of the molecular mechanisms underlying th…
View article: Evaluating signaling pathway inference from kinase-substrate interactions and phosphoproteomics data
Evaluating signaling pathway inference from kinase-substrate interactions and phosphoproteomics data Open
Cellular signaling plays a vital role in how cells communicate and adapt to both environmental and internal cues. At the molecular level, signaling is largely driven by phosphorylation cascades controlled by kinases. Because of this, kinas…
View article: Subcellular thermal profiling enables the deep functional exploration of the mitochondrial proteome
Subcellular thermal profiling enables the deep functional exploration of the mitochondrial proteome Open
Mitochondria are membrane-bound organelle hubs of cellular metabolism and signaling. The dysregulation of mitochondria is related to the genesis of several highly prevalent diseases, including cancer and cardiovascular disorders, urging th…
View article: Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response
Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response Open
View article: Deep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome
Deep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome Open
Highlights High throughput glycoproteomics method with multiplexed quantification 25-fold improvement of the mouse brain glycoproteome coverage Structural features dictate level of glycosite micro-heterogeneity Gut microbiome composition e…
View article: Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response
Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response Open
A hallmark of animals is the coordination of whole-body movement. Neurons and muscles are central to this, yet coordinated movements also exist in sponges that lack these cell types. Sponges are sessile animals with a complex canal system …
View article: Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response
Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response Open
A hallmark of animals is the coordination of whole-body movement. Neurons and muscles are central to this, yet coordinated movements also exist in sponges that lack these cell types. Sponges are sessile animals with a complex canal system …
View article: Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response
Molecular profiling of sponge deflation reveals an ancient relaxant-inflammatory response Open
A hallmark of animals is the coordination of whole-body movement. Neurons and muscles are central to this, yet coordinated movements also exist in sponges that lack these cell types. Sponges are sessile animals with a complex canal system …
View article: Dual truncation of tau by caspase-2 accelerates its CHIP-mediated degradation
Dual truncation of tau by caspase-2 accelerates its CHIP-mediated degradation Open
Intraneuronal aggregates of the microtubule binding protein Tau are a hallmark of different neurodegenerative diseases including Alzheimer's disease (AD). In these aggregates, Tau is modified by posttranslational modifications such as phos…
View article: Dextromethorphan inhibits collagen transport in the endoplasmic reticulum eliciting an anti-fibrotic response in<i>ex-vivo</i>and<i>in vitro</i>models of pulmonary fibrosis
Dextromethorphan inhibits collagen transport in the endoplasmic reticulum eliciting an anti-fibrotic response in<i>ex-vivo</i>and<i>in vitro</i>models of pulmonary fibrosis Open
Excessive deposition of fibrillar collagen in the interstitial extracellular matrix (ECM) of human lung tissue causes fibrosis, which can ultimately lead to organ failure. Despite our understanding of the molecular mechanisms underlying th…
View article: Molecular mechanisms of stress-induced reactivation in mumps virus condensates
Molecular mechanisms of stress-induced reactivation in mumps virus condensates Open
Negative-stranded RNA viruses can establish long-term persistent infection in the form of large intracellular inclusions in the human host and cause chronic diseases. Here, we uncover how cellular stress disrupts the metastable host-virus …
View article: Deep thermal profiling for detection of functional proteoform groups
Deep thermal profiling for detection of functional proteoform groups Open
View article: Author response: Defining basic rules for hardening influenza A virus liquid condensates
Author response: Defining basic rules for hardening influenza A virus liquid condensates Open
Full text Figures and data Side by side Abstract Editor's evaluation eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abs…
View article: Defining basic rules for hardening influenza A virus liquid condensates_data and scripts
Defining basic rules for hardening influenza A virus liquid condensates_data and scripts Open
In biological systems, liquid and solid-like biomolecular condensates may contain the same molecules but their behaviour, including movement, elasticity and viscosity, is different on account of distinct physicochemical properties. As such…
View article: Rules for hardening influenza a virus liquid condensates: Data and Codes
Rules for hardening influenza a virus liquid condensates: Data and Codes Open
Controlled phase transitions in biomolecular condensates will provide further understanding to cell physiology and diseases as the material properties in these structures are tailored for function. Since native or engineered transitions af…
View article: Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkers
Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkers Open
The complexity of the functional proteome extends significantly beyond the protein coding genome resulting in millions of proteoforms. Investigation of proteoforms and their functional roles is important to understand cellular physiology a…
View article: Author Correction: A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles
Author Correction: A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles Open
View article: Isocotoin suppresses hepatitis E virus replication through inhibition of heat shock protein 90
Isocotoin suppresses hepatitis E virus replication through inhibition of heat shock protein 90 Open
View article: A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles
A computational method for detection of ligand-binding proteins from dose range thermal proteome profiles Open
View article: Computational analysis of ligand dose range thermal proteome profiles
Computational analysis of ligand dose range thermal proteome profiles Open
Detecting ligand-protein interactions in living cells is a fundamental challenge in molecular biology and drug research. Proteome-wide profiling of thermal stability as a function of ligand concentration promises to tackle this challenge. …
View article: Impact of phosphorylation on thermal stability of proteins
Impact of phosphorylation on thermal stability of proteins Open
Summary Reversible protein phosphorylation regulates virtually every cellular process and is arguably the most well-studied post-translational modification. Still, less than 3% of the phosphorylation sites identified in humans have annotat…
View article: Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis
Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis Open
Protein degradation plays important roles in biological processes and is tightly regulated. Further, targeted proteolysis is an emerging research tool and therapeutic strategy. However, proteome-wide technologies to investigate the causes …
View article: Systematic analysis of protein turnover in primary cells
Systematic analysis of protein turnover in primary cells Open
A better understanding of proteostasis in health and disease requires robust methods to determine protein half-lives. Here we improve the precision and accuracy of peptide ion intensity-based quantification, enabling more accurate protein …
View article: Functional interdependence of BRD4 and DOT1L in MLL leukemia
Functional interdependence of BRD4 and DOT1L in MLL leukemia Open