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View article: Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum Open
View article: Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum
Molecular characterization of the missing electron pathways for butanol synthesis in Clostridium acetobutylicum Open
Clostridium acetobutylicum is a promising biocatalyst for the production of n-butanol at high yield from renewable resources. Several metabolic strategies have already been developed to increase butanol yields, most often based on carbon p…
View article: Insights into Clostridium tetani: From genome to bioreactors
Insights into Clostridium tetani: From genome to bioreactors Open
View article: Author Correction: The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster
Author Correction: The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster Open
View article: An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors Open
View article: An Overview of the Ferredoxin NAD+ Reductases Used for Energy Conservation in Various Anaerobic Microorganisms
An Overview of the Ferredoxin NAD+ Reductases Used for Energy Conservation in Various Anaerobic Microorganisms Open
In the context of the development of bioprocesses for the production of biofuels and bulk chemicals, microbial cells are rationally engineered to produce such molecules at high yield and titres in order to develop new biological methods th…
View article: Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O<sub>2</sub> Resistance and Catalytic Bias?
Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O<sub>2</sub> Resistance and Catalytic Bias? Open
[FeFe]-hydrogenases, HydAs, are unique biocatalysts for proton reduction to H2. However, they suffer from a number of drawbacks for biotechnological applications: size, number and diversity of metal cofactors, oxygen sensitivity…
View article: Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases
Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases Open
FeFe hydrogenases catalyze H2 oxidation and formation at an inorganic active site (the "H-cluster"), which consists of a [Fe2(CO)3(CN)2(dithiomethylamine)] subcluster covalently attached to a Fe<…
View article: Mechanism of O2 diffusion and reduction in FeFe hydrogenases
Mechanism of O2 diffusion and reduction in FeFe hydrogenases Open
View article: Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach Open
View article: Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum
Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum Open
View article: MOESM2 of Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
MOESM2 of Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach Open
Additional file 2. Dataset S1. Transcriptomic data of the total open reading frames (ORFs).
View article: Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel
Impact of the chemicals, essential for the purification process of strict Fe-hydrogenase, on the corrosion of mild steel Open
View article: A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum
A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum Open
Engineering industrial microorganisms for ambitious applications, for example, the production of second-generation biofuels such as butanol, is impeded by a lack of knowledge of primary metabolism and its regulation. A quantitative system-…
View article: Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O<sub>2</sub> Demonstrate That the Reaction Is Partly Reversible
Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O<sub>2</sub> Demonstrate That the Reaction Is Partly Reversible Open
The mechanism of reaction of FeFe hydrogenases with oxygen has been debated. It is complex, apparently very dependent on the details of the protein structure, and difficult to study using conventional kinetic techniques. Here we build on o…
View article: Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum
Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum Open
Our study shows that the cellulolytic capacity of R. cellulolyticum can be augmented by supplementing the cellulosomes with a free cellulase originating from L. phytofermentans, whereas integration of the heterologous enzyme in the cellulo…