Issei Nishiki
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View article: Complete Genome Sequence of <i>Weissella tructae</i> Strain TB02, a Pathogen Isolated from Disease Rainbow Trout (<i>Oncorhynchus mykiss</i>) in Vietnam
Complete Genome Sequence of <i>Weissella tructae</i> Strain TB02, a Pathogen Isolated from Disease Rainbow Trout (<i>Oncorhynchus mykiss</i>) in Vietnam Open
View article: Emergence of Erythromycin-resistant <i>Lactococcus garvieae</i> Serotype III Carrying <i>erm</i>(B) Isolated from Striped Jack <i>Pseudocaranx dentex</i>
Emergence of Erythromycin-resistant <i>Lactococcus garvieae</i> Serotype III Carrying <i>erm</i>(B) Isolated from Striped Jack <i>Pseudocaranx dentex</i> Open
View article: Characterization and Epidemiological Study of Newly Emerging <i>Lactococcus garvieae</i> Serotype III in Farmed Fish in Japan
Characterization and Epidemiological Study of Newly Emerging <i>Lactococcus garvieae</i> Serotype III in Farmed Fish in Japan Open
View article: Development of a Discriminating Method for Pathogenic Bacteria that Cause Lactococcal Infections in Marine Fish Farms in Japan
Development of a Discriminating Method for Pathogenic Bacteria that Cause Lactococcal Infections in Marine Fish Farms in Japan Open
View article: IncX3 plasmid carrying blaSHV-12 and qnrS1 in a Japanese racehorse-origin Escherichia coli isolate
IncX3 plasmid carrying blaSHV-12 and qnrS1 in a Japanese racehorse-origin Escherichia coli isolate Open
Plasmids play an important role in the spread of antimicrobial resistance genes (ARGs) in bacteria. This study reports the complete sequence of the IncX3 plasmid identified in Escherichia coli isolated from faeces of a Japanese racehorse. …
View article: Emerging New Serotype <i>Lactococcus garvieae </i>Infection in Farmed Marine Fish in Japan
Emerging New Serotype <i>Lactococcus garvieae </i>Infection in Farmed Marine Fish in Japan Open
Lactococcus garvieaeⅠ型およびⅡ型を含む市販の混合ワクチンを投与した宮崎県の養殖カンパチで,2021年8月からL. garvieae感染症に類似した症状を呈する疾病が確認されるようになった。分離された菌株は,L. garvieae診断用抗Ⅰ型およびⅡ型血清に対して非凝集性を示し,多遺伝子座配列解析(MLSA)においてL. garvieaeⅠ型に近く,Ⅱ型とは遺伝学的相違性が認められた。同菌株を用いたカンパチに対する腹腔内接種による感染試験では,半数致死濃…
View article: Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of <i>Nocardia seriolae</i>
Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of <i>Nocardia seriolae</i> Open
Lytic bacteriophage NS-I shows promise as a biocontrol agent for nocardiosis infection in fish farms and warrants further investigation.
View article: A Single Point Mutation in the <i>lsa</i>(D) Variant in Fish Pathogenic <i>Lactococcus garvieae</i> Serotype I Which Affects Lincomycin and Related Antibiotics Resistance
A Single Point Mutation in the <i>lsa</i>(D) Variant in Fish Pathogenic <i>Lactococcus garvieae</i> Serotype I Which Affects Lincomycin and Related Antibiotics Resistance Open
This study aimed to elucidate the genetic characteristics of lincomycin (LCM) resistance in the absence of erythromycin (EM) resistance in fish pathogenic Lactococcus garvieae serotype I. Two novel lsa(D) variants were found in clinically…
View article: <i>Vibrio anguillarum</i> J-O-1 Type Infection in Farmed Greater Amberjack <i>Seriola dumerili</i>
<i>Vibrio anguillarum</i> J-O-1 Type Infection in Farmed Greater Amberjack <i>Seriola dumerili</i> Open
市販のJ-O-3型ビブリオ病ワクチンを投与した養殖カンパチで眼球突出，眼球白濁，頭部上皮組織の剥離および鰓蓋発赤の症状を呈する疾病が発生した。死亡魚から分離した細菌はAPE20E，抗Vibrio anguillarum J-O-1 ウサギ血清への凝集反応，ビブリオ属細菌同定用マルチプレックスPCRおよび16S rRNA配列の解析からV. anguillarum J-O-1型に同定された。分離細菌は感染実験によりカンパチに病原性を示すことが確認された。また，分離細菌を用いて作…
View article: Complete Genome Sequence of Lactococcus garvieae MS210922A, Isolated from Farmed Greater Amberjack (Seriola dumerili) in Japan
Complete Genome Sequence of Lactococcus garvieae MS210922A, Isolated from Farmed Greater Amberjack (Seriola dumerili) in Japan Open
Here, we report the complete genome sequence of a potentially new serotype, Lactococcus garvieae strain MS210922A, isolated from greater amberjack. It is nonreactive to serotype I or II antisera. The complete genome comprises 2,007,159 bp,…
View article: Author Correction: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
Author Correction: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis) Open
View article: Author Correction: A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone
Author Correction: A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone Open
View article: A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone
A novel Asfarvirus-like virus identified as a potential cause of mass mortality of abalone Open
A novel Asfarvirus-like virus is proposed as the etiological agent responsible for mass mortality in abalone. The disease, called abalone amyotrophia, originally was recognized in the 1980s, but efforts to identify a causative agent were u…
View article: Improvement of the Pacific bluefin tuna (Thunnus orientalis) reference genome and development of male-specific DNA markers
Improvement of the Pacific bluefin tuna (Thunnus orientalis) reference genome and development of male-specific DNA markers Open
View article: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis) Open
View article: Epidemiological Study on Newly Emerging <i>Lactococcus garvieae</i> Serotype II Isolated from Marine Fish Species in Japan
Epidemiological Study on Newly Emerging <i>Lactococcus garvieae</i> Serotype II Isolated from Marine Fish Species in Japan Open
Lactococcus garvieae serotype II, a newly emerging causal pathogen of lactococcal infection, has spread to fish farms since 2012, and its damage to aquaculture has increased. A total of 161 strains were isolated from four fish species, ye…
View article: Complete genome sequence and characterization of virulence genes in Lancefield group C <i>Streptococcus dysgalactiae</i> isolated from farmed amberjack (<i>Seriola dumerili</i>)
Complete genome sequence and characterization of virulence genes in Lancefield group C <i>Streptococcus dysgalactiae</i> isolated from farmed amberjack (<i>Seriola dumerili</i>) Open
Lancefield group C Streptococcus dysgalactiae causes infections in farmed fish. Here, the genome of S. dysgalactiae strain kdys0611, isolated from farmed amberjack ( Seriola dumerili ) was sequenced. The complete genome sequence of kdys061…
View article: Genetic parameters and quantitative trait loci analysis associated with body size and timing at metamorphosis into glass eels in captive-bred Japanese eels (Anguilla japonica)
Genetic parameters and quantitative trait loci analysis associated with body size and timing at metamorphosis into glass eels in captive-bred Japanese eels (Anguilla japonica) Open
The Japanese eel (Anguilla japonica) is among the most important aquaculture fish species in Eastern Asia. The present study aimed to identify the genetic parameters underlying body size and the timing at metamorphosis from leptocephali to…
View article: The yellowtail (<i>Seriola quinqueradiata</i>) genome and transcriptome atlas of the digestive tract
The yellowtail (<i>Seriola quinqueradiata</i>) genome and transcriptome atlas of the digestive tract Open
Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture in Japan. In this study, we used the genome of haploid yellowtail fish larvae for de novo assembly of whole-genome sequences, and …
View article: Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing
Identification of genetic linkage group 1-linked sequences in Japanese eel (Anguilla japonica) by single chromosome sorting and sequencing Open
Japanese eel (Anguilla japonica) constitutes one of the most important food fish in Japan; accordingly, genome sequencing and linkage mapping have been conducted for the purpose of artificial cultivation. In the next stage, integration of …
View article: Isolation of <i>Streptococcus dysgalactiae</i> from farmed striped jack <i>Pseudocaranx dentex</i>
Isolation of <i>Streptococcus dysgalactiae</i> from farmed striped jack <i>Pseudocaranx dentex</i> Open
2015年夏季に養殖シマアジ病魚からランスフィールドC群Streptococcus dysgalactiae（GCSD）が宮崎県内で初めて分離された。シマアジから分離されたGCSDはカンパチやブリから分離されたGCSDと同様の生化学的性状を示し，GCSD同定用のPCRにおいても陽性であった。また，攻撃実験により，シマアジ由来GCSD株のシマアジに対する病原性が確認された。本報はシマアジにおけるGCSD感染症国内症例の初報告である。
View article: Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species
Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species Open
Nocardiosis caused by Nocardia seriolae is one of the major threats in the aquaculture of Seriola species (yellowtail; S. quinqueradiata, amberjack; S. dumerili and kingfish; S. lalandi) in Japan. Here, we report the complete nucleotide ge…
View article: PCR-mediated Identification of the Newly Emerging Pathogen <i>Lactococcus garvieae</i> Serotype II from <i>Seriola quinqueradiata</i> and <i>S. dumerili</i>
PCR-mediated Identification of the Newly Emerging Pathogen <i>Lactococcus garvieae</i> Serotype II from <i>Seriola quinqueradiata</i> and <i>S. dumerili</i> Open
The newly emerging pathogen Lactococcus garvieae serotype II, which appears to be morphologically and biochemically indistinguishable from L. garvieae serotype I, has been isolated from farmed Seriola quinqueradiata and S. dumerili. To dis…
View article: Complete Genome Sequence of Nonagglutinating <i>Lactococcus garvieae</i> Strain 122061 Isolated from Yellowtail in Japan
Complete Genome Sequence of Nonagglutinating <i>Lactococcus garvieae</i> Strain 122061 Isolated from Yellowtail in Japan Open
Nonagglutinating Lactococcus garvieae has been isolated from diseased farmed yellowtail in Japan since 2012. In this study, the complete genome and plasmid sequence of nonagglutinating L. garvieae strain 122061 was determined, to our knowl…
View article: Effective de novo assembly of fish genome using haploid larvae
Effective de novo assembly of fish genome using haploid larvae Open
Recent improvements in next-generation sequencing technology have made it possible to do whole genome sequencing, on even non-model eukaryote species with no available reference genomes. However, de novo assembly of diploid genomes is stil…
View article: A functional genomics tool for the Pacific bluefin tuna: Development of a 44K oligonucleotide microarray from whole-genome sequencing data for global transcriptome analysis
A functional genomics tool for the Pacific bluefin tuna: Development of a 44K oligonucleotide microarray from whole-genome sequencing data for global transcriptome analysis Open
Bluefin tunas are one of the most important fishery resources worldwide. Because of high market values, bluefin tuna farming has been rapidly growing during recent years. At present, the most common form of the tuna farming is based on the…
View article: V-GAP: Viral genome assembly pipeline
V-GAP: Viral genome assembly pipeline Open
Next-generation sequencing technologies have allowed the rapid determination of the complete genomes of many organisms. Although shotgun sequences from large genome organisms are still difficult to reconstruct perfect contigs each of which…