Ivo Steinmetz
YOU?
Author Swipe
View article: Melioidosis in Mali: a retrospective observational study
Melioidosis in Mali: a retrospective observational study Open
For the French translation of the abstract see Supplementary Materials section.
View article: Identification of Filamentous Fungi: An Evaluation of Three <scp>MALDI</scp>‐<scp>TOF</scp> Mass Spectrometry Systems
Identification of Filamentous Fungi: An Evaluation of Three <span>MALDI</span>‐<span>TOF</span> Mass Spectrometry Systems Open
Background Identification of filamentous fungi still poses a major challenge to laboratories. Matrix assisted laser desorption/ionisation time of flight mass spectrometry (MALDI‐TOF MS) is a promising tool, since it offers low‐cost and fas…
View article: Anti-RBD immunoglobulin levels and their predictive value for SARS-CoV-2 neutralization
Anti-RBD immunoglobulin levels and their predictive value for SARS-CoV-2 neutralization Open
A large number of studies have demonstrated that anti-receptor-binding domain (RBD)-binding antibody titers correlate with SARS-CoV-2 neutralization and protection from the disease. Unlike live virus neutralization assays, antibody-binding…
View article: Improving Nanopore sequencing-based core genome MLST for global infection control: a strategy for GC-rich pathogens like <i>Burkholderia pseudomallei</i>
Improving Nanopore sequencing-based core genome MLST for global infection control: a strategy for GC-rich pathogens like <i>Burkholderia pseudomallei</i> Open
Genomic surveillance of pathogens is essential to trace infections and analyze resistance markers. Core genome multilocus sequence typing (cgMLST) facilitates genomic surveillance by simplified analysis and standardization. However, its ap…
View article: Newly discovered paediatric melioidosis in Mali: the tip of an African iceberg?
Newly discovered paediatric melioidosis in Mali: the tip of an African iceberg? Open
View article: Colistin, Meropenem–Vaborbactam, Imipenem–Relebactam, and Eravacycline Testing in Carbapenem-Resistant Gram-Negative Rods: A Comparative Evaluation of Broth Microdilution, Gradient Test, and VITEK 2
Colistin, Meropenem–Vaborbactam, Imipenem–Relebactam, and Eravacycline Testing in Carbapenem-Resistant Gram-Negative Rods: A Comparative Evaluation of Broth Microdilution, Gradient Test, and VITEK 2 Open
Objectives. This study aimed to evaluate and compare the performance of different assays for antimicrobial susceptibility testing (AST) and minimum inhibitory concentration (MIC) determination for reserve antibiotics in carbapenem-resistan…
View article: A comparison of two MALDI-TOF MS based assays for the detection of carbapenemases in Enterobacterales
A comparison of two MALDI-TOF MS based assays for the detection of carbapenemases in Enterobacterales Open
Carbapenem resistant (CRE) and carbapenemase producing Enterobacterales (CPE) in particular, represent a major threat for healthcare systems worldwide. Rapid, reliable, and easy to perform assays are required to enable targeted and effecti…
View article: A multicenter study on accuracy and reproducibility of nanopore sequencing-based genotyping of bacterial pathogens
A multicenter study on accuracy and reproducibility of nanopore sequencing-based genotyping of bacterial pathogens Open
Nanopore sequencing has shown the potential to democratize genomic pathogen surveillance due to its ease of use and low entry cost. However, recent genotyping studies showed discrepant results compared to gold-standard short-read sequencin…
View article: Core genome multilocus sequence typing (cgMLST) applicable to the monophyletic <i>Klebsiella oxytoca</i> species complex
Core genome multilocus sequence typing (cgMLST) applicable to the monophyletic <i>Klebsiella oxytoca</i> species complex Open
The environmental bacterium Klebsiella oxytoca displays an alarming increase of antibiotic-resistant strains that frequently cause outbreaks in intensive care units. Due to its prevalence in the environment and opportunistic presence in hu…
View article: The hidden burden of melioidosis in Nepal: a paradigm for the urgent need to implement a simple laboratory algorithm to detect Burkholderia pseudomallei in low-resource endemic areas
The hidden burden of melioidosis in Nepal: a paradigm for the urgent need to implement a simple laboratory algorithm to detect Burkholderia pseudomallei in low-resource endemic areas Open
Melioidosis, an emerging infectious disease caused by the Gram-negative bacillus Burkholderia pseudomallei, is massively underdiagnosed in many low- and middle-income countries. The disease is clinically extremely variable, has a hi…
View article: Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison
Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison Open
View article: Bacitracin agar vs. oleandomycin disk supplemented chocolate agar for the recovery of Haemophilus influenzae in diagnostic samples: A prospective comparison
Bacitracin agar vs. oleandomycin disk supplemented chocolate agar for the recovery of Haemophilus influenzae in diagnostic samples: A prospective comparison Open
Haemophilus influenzae is an important pathogen able to cause various forms of respiratory and invasive disease. To provide high sensitivity for detection, culture media must inhibit growth of residential flora from the respiratory tract. …
View article: Vaccination Versus Hybrid Immunization: Sera with Similar Anti-Rbd Total IG Units Show Different Virus Neutralization Capacity
Vaccination Versus Hybrid Immunization: Sera with Similar Anti-Rbd Total IG Units Show Different Virus Neutralization Capacity Open
View article: Deciphering the human antibody response against Burkholderia pseudomallei during melioidosis using a comprehensive immunoproteome approach
Deciphering the human antibody response against Burkholderia pseudomallei during melioidosis using a comprehensive immunoproteome approach Open
Introduction The environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis. Antigens inducing protective immunity in experimental models have recently been identifie…
View article: A head-to-head comparison of three MALDI-TOF mass spectrometry systems with 16S rRNA gene sequencing
A head-to-head comparison of three MALDI-TOF mass spectrometry systems with 16S rRNA gene sequencing Open
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized diagnostics in culture-based microbiology. Commonly used MALDI-TOF MS systems in clinical microbiology laboratories are MALDI Bi…
View article: Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogen Burkholderia pseudomallei from soil samples
Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogen Burkholderia pseudomallei from soil samples Open
The soil bacterium Burkholderia pseudomallei causes melioidosis, a potentially fatal and greatly underdiagnosed tropical disease. Detection of B. pseudomallei in the environment is important to trace the source of infections, define risk a…
View article: Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance
Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance Open
Next-generation whole-genome sequencing is essential for high-resolution surveillance of bacterial pathogens, for example, during outbreak investigations or for source tracking and escape variant analysis. However, current global sequencin…
View article: Newly detected pediatric melioidosis cases in a single referral children’s hospital in Ho Chi Minh City indicate the probable under-recognition of melioidosis in South Vietnam
Newly detected pediatric melioidosis cases in a single referral children’s hospital in Ho Chi Minh City indicate the probable under-recognition of melioidosis in South Vietnam Open
Purpose. The epidemiology of melioidosis in Vietnam, a disease caused by the soil bacterium Burkholderia pseudomallei , remains unclear . This study aimed to detect pediatric melioidosis in South Vietnam and describe clinical features and …
View article: Complete Genome Sequence of Klebsiella oxytoca Strain AHC-6, Isolated from a Patient during Acute Antibiotic-Associated Hemorrhagic Colitis
Complete Genome Sequence of Klebsiella oxytoca Strain AHC-6, Isolated from a Patient during Acute Antibiotic-Associated Hemorrhagic Colitis Open
Klebsiella oxytoca is a ubiquitous bacterium that is increasingly associated with inflammatory diseases. Here, we report the hybrid assembled genome for cytotoxic K. oxytoca strain AHC-6. The genome comprises a total of 5.7 Mbp, with a GC …
View article: Altered cellular immune response to vaccination against SARS-CoV-2 in patients suffering from autoimmunity with B-cell depleting therapy
Altered cellular immune response to vaccination against SARS-CoV-2 in patients suffering from autoimmunity with B-cell depleting therapy Open
View article: Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics
Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics Open
Burkholderia mallei , the causative agent of glanders, is a clonal descendant of Burkholderia pseudomallei , the causative agent of melioidosis, which has lost its environmental reservoir and has a restricted host range. Despite limitation…
View article: <scp>S</scp> evere acute respiratory syndrome coronavirus 2 spike antibody level decline is more pronounced after the second vaccination, but response to the third vaccination is similar in people with type 1 and type 2 diabetes compared with healthy controls: The prospective <scp>COVAC‐DM</scp> cohort study
<span>S</span> evere acute respiratory syndrome coronavirus 2 spike antibody level decline is more pronounced after the second vaccination, but response to the third vaccination is similar in people with type 1 and type 2 diabetes compared with healthy controls: The prospective <span>COVAC‐DM</span> cohort study Open
Up to 1 June 2022, almost 12 billion vaccine doses for coronavirus disease 2019 (COVID-19) were administered worldwide.1 People with diabetes were more probable to be hospitalized for COVID-19 and were observed to have in-hospital mortalit…
View article: Lower Levels of ABO Anti-A and Anti-B of IgM, IgG and IgA Isotypes in the Serum but Not the Saliva of COVID-19 Convalescents
Lower Levels of ABO Anti-A and Anti-B of IgM, IgG and IgA Isotypes in the Serum but Not the Saliva of COVID-19 Convalescents Open
Individuals with ABO type O, naturally possessing anti-A and anti-B antibodies in their serum, are underrepresented among patients infected with SARS-CoV-2 compared with healthy controls. The ABO antibodies might play a role in the viral t…
View article: Environmental Factors Associated With Soil Prevalence of the Melioidosis Pathogen Burkholderia pseudomallei: A Longitudinal Seasonal Study From South West India
Environmental Factors Associated With Soil Prevalence of the Melioidosis Pathogen Burkholderia pseudomallei: A Longitudinal Seasonal Study From South West India Open
Melioidosis is a seasonal infectious disease in tropical and subtropical areas caused by the soil bacterium Burkholderia pseudomallei. In many parts of the world, including South West India, most cases of human infections are reported duri…
View article: Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout
Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout Open
Measuring SARS-CoV-2 neutralizing antibodies after vaccination or natural infection remains a priority in the ongoing COVID-19 pandemic to determine immunity, especially against newly emerging variants. The gold standard for assessing anti…
View article: Open-Source, Adaptable, All-in-One Smartphone-Based System for Quantitative Analysis of Point-of-Care Diagnostics
Open-Source, Adaptable, All-in-One Smartphone-Based System for Quantitative Analysis of Point-of-Care Diagnostics Open
Point-of-care (POC) diagnostics, in particular lateral flow assays (LFA), represent a great opportunity for rapid, precise, low-cost and accessible diagnosis of disease. Especially with the ongoing coronavirus disease 2019 (COVID-19) pande…
View article: Humoral immune response to <scp>COVID‐19</scp> vaccination in diabetes is age‐dependent but independent of type of diabetes and glycaemic control: The prospective <scp>COVAC‐DM</scp> cohort study
Humoral immune response to <span>COVID‐19</span> vaccination in diabetes is age‐dependent but independent of type of diabetes and glycaemic control: The prospective <span>COVAC‐DM</span> cohort study Open
Aims To investigate the seroconversion following first and second COVID‐19 vaccination in people with type 1 and type 2 diabetes in relation to glycaemic control prior to vaccination and to analyse the response in comparison to individuals…
View article: A Novel High-Throughput Nanopore-Sequencing-Based Strategy for Rapid and Automated S-Protein Typing of SARS-CoV-2 Variants
A Novel High-Throughput Nanopore-Sequencing-Based Strategy for Rapid and Automated S-Protein Typing of SARS-CoV-2 Variants Open
Rapid molecular surveillance of SARS-CoV-2 S-protein variants leading to immune escape and/or increased infectivity is of utmost importance. Among global bottlenecks for variant monitoring in diagnostic settings are sequencing and bioinfor…
View article: Author response for "Humoral immune response to Covid‐19 vaccination in diabetes: age‐dependent but independent of type of diabetes and glycaemic control ‐ the prospective COVAC‐DM cohort study"
Author response for "Humoral immune response to Covid‐19 vaccination in diabetes: age‐dependent but independent of type of diabetes and glycaemic control ‐ the prospective COVAC‐DM cohort study" Open
View article: CD19+IgD+CD27- Naïve B Cells as Predictors of Humoral Response to COVID 19 mRNA Vaccination in Immunocompromised Patients
CD19+IgD+CD27- Naïve B Cells as Predictors of Humoral Response to COVID 19 mRNA Vaccination in Immunocompromised Patients Open
Immunocompromised patients are considered high-risk and prioritized for vaccination against COVID-19. We aimed to analyze B-cell subsets in these patients to identify potential predictors of humoral vaccination response. Patients (n=120) s…