Iyadh Douagi
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View article: Leveraging optimized oligonucleotide-tagged antigen assemblies and single-cell sequencing for multiplexed proteogenomic profiling of human B cell reactivities
Leveraging optimized oligonucleotide-tagged antigen assemblies and single-cell sequencing for multiplexed proteogenomic profiling of human B cell reactivities Open
Antigen-specific responses to complex antigens encompass a range of cell states and reactivities to an array of epitopes, reflective of the heterogeneity in immune responses. Single-cell sequencing has created new opportunities when combin…
View article: Bismuth subsalicylate profoundly alters gut microbiome and immunity with increased susceptibility to infection
Bismuth subsalicylate profoundly alters gut microbiome and immunity with increased susceptibility to infection Open
Bismuth subsalicylate (BSS) is a commonly used over-the-counter medication for a variety of gastrointestinal symptoms. BSS sequesters gut sulfides, which have been shown in murine studies to be key regulators of gut microbiota. Here, we in…
View article: Antibody-Mediated Inhibition of HLA/LILR Interactions Breaks Innate Immune Tolerance and Induces Antitumor Immunity
Antibody-Mediated Inhibition of HLA/LILR Interactions Breaks Innate Immune Tolerance and Induces Antitumor Immunity Open
Immune checkpoint blockade for the treatment of malignancies has been focused on reversing inhibitory pathways in T lymphocytes. NK cells are a potent innate defense against tumors and virally infected cells, but their therapeutic manipula…
View article: Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples
Verifying Proper Function of the Aerosol Evacuation System Prior to Sorting Potentially Infectious Samples Open
High concentrations of aerosols can be generated within the sort collection area of cell sorters during instrument failures that cause the stream to deviate, such as a partial nozzle obstruction. Complete containment of these aerosol parti…
View article: Gut microbiome shifts in adolescents after sleeve gastrectomy with increased oral-associated taxa and pro-inflammatory potential
Gut microbiome shifts in adolescents after sleeve gastrectomy with increased oral-associated taxa and pro-inflammatory potential Open
Bariatric surgery is highly effective in achieving weight loss in children and adolescents with severe obesity, however the underlying mechanisms are incompletely understood, and gut microbiome changes are unknown. Here, we show that adole…
View article: Gut microbiome shifts in adolescents after sleeve gastrectomy with increased oral-associated taxa and pro-inflammatory potential
Gut microbiome shifts in adolescents after sleeve gastrectomy with increased oral-associated taxa and pro-inflammatory potential Open
Background Bariatric surgery is highly effective in achieving weight loss in children and adolescents with severe obesity, however the underlying mechanisms are incompletely understood, and gut microbiome changes are unknown. Objectives 1)…
View article: R406 reduces lipopolysaccharide-induced neutrophil activation
R406 reduces lipopolysaccharide-induced neutrophil activation Open
Modulating SYK has been demonstrated to have impacts on pathogenic neutrophil responses in COVID-19. During sepsis, neutrophils are vital in early bacterial clearance but also contribute to the dysregulated immune response and organ injury…
View article: Conjugation of Fluorochromes to Monoclonal Antibodies
Conjugation of Fluorochromes to Monoclonal Antibodies Open
Detection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is the most widely used application of flow cytometry. Here, we present protocols for tagging monoclonal antibodies with fluore…
View article: Peripheral death by neglect and limited clonal deletion during physiologic B lymphocyte development
Peripheral death by neglect and limited clonal deletion during physiologic B lymphocyte development Open
Autoreactive B cells generated during B cell development are inactivated by clonal deletion, receptor editing or anergy. Up to 97% of immature B cells appear to die before completing maturation, but the anatomic sites and reasons underlyin…
View article: Broadly neutralizing antibodies target the coronavirus fusion peptide
Broadly neutralizing antibodies target the coronavirus fusion peptide Open
The potential for future coronavirus outbreaks highlights the need to broadly target this group of pathogens. We used an epitope-agnostic approach to identify six monoclonal antibodies that bind to spike proteins from all seven human-infec…
View article: Broadly neutralizing antibodies target the coronavirus fusion peptide
Broadly neutralizing antibodies target the coronavirus fusion peptide Open
The potential for future coronavirus outbreaks highlights the need to develop strategies and tools to broadly target this group of pathogens. Here, using an epitope-agnostic approach, we identified six monoclonal antibodies that bound to s…
View article: Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern
Bispecific antibodies targeting distinct regions of the spike protein potently neutralize SARS-CoV-2 variants of concern Open
Bispecific antibodies targeting multiple regions of the SARS-CoV-2 spike protein comparably neutralize variants of concern and wild-type virus.
View article: Ultrapotent bispecific antibodies neutralize emerging SARS-CoV-2 variants
Ultrapotent bispecific antibodies neutralize emerging SARS-CoV-2 variants Open
The emergence of SARS-CoV-2 variants that threaten the efficacy of existing vaccines and therapeutic antibodies underscores the urgent need for new antibody-based tools that potently neutralize variants by targeting multiple sites of the s…
View article: Molecular profiling of retinal pigment epithelial cell differentiation for therapeutic use
Molecular profiling of retinal pigment epithelial cell differentiation for therapeutic use Open
Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) are a promising cell source to treat age-related macular degeneration (AMD). Despite several ongoing clinical studies, detailed single cell mapping of the transi…
View article: Risk awareness during operation of analytical flow cytometers and implications throughout the <scp>COVID</scp>‐19 pandemic
Risk awareness during operation of analytical flow cytometers and implications throughout the <span>COVID</span>‐19 pandemic Open
The COVID‐19 pandemic has brought biosafety to the forefront of many life sciences. The outbreak has compelled research institutions to re‐evaluate biosafety practices and potential at‐risk areas within research laboratories and more speci…
View article: Biosafety during a pandemic: shared resource laboratories rise to the challenge
Biosafety during a pandemic: shared resource laboratories rise to the challenge Open
Biosafety has always been an important aspect of daily work in any research institution, particularly for cytometry Shared Resources Laboratories (SRLs). SRLs are common‐use spaces that facilitate the sharing of knowledge, expertise, and i…
View article: Absence of a common founder mutation in patients with cooccurring myelodysplastic syndrome and plasma cell disorder
Absence of a common founder mutation in patients with cooccurring myelodysplastic syndrome and plasma cell disorder Open
Epidemiological studies have demonstrated an increased incidence of MDS in patients with plasma cell disorder (PCD) i.e. multiple myeloma (MM) or MGUS and several case reports / series of co-occurring MDS and PCD have been published. The u…
View article: Publisher Correction: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells
Publisher Correction: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells Open
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View article: Publisher Correction: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells
Publisher Correction: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells Open
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
View article: OR31-03 Single-Cell Profiling of Adult Human Ovarian Cortex Reveals Six Main Cell Types but No Germline Stem Cells
OR31-03 Single-Cell Profiling of Adult Human Ovarian Cortex Reveals Six Main Cell Types but No Germline Stem Cells Open
The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve.…
View article: Xeno-free, chemically defined and scalable monolayer differentiation protocol for retinal pigment epithelial cells
Xeno-free, chemically defined and scalable monolayer differentiation protocol for retinal pigment epithelial cells Open
With cell therapies advancing towards the patients’ bedside, there is an increased need for robust and up-scalable protocols to generate clinical grade stem cell-derived products.The protocol we present in the study provides a xeno-free, c…
View article: Xeno-free, chemically defined and scalable monolayer differentiation protocol for retinal pigment epithelial cells
Xeno-free, chemically defined and scalable monolayer differentiation protocol for retinal pigment epithelial cells Open
With cell therapies advancing towards the patients’ bedside, there is an increased need for robust and up-scalable protocols to generate clinical grade stem cell-derived products.The protocol we present in the study provides a xeno-free, c…