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View article: Unique double-helical packing of protein molecules in the crystal of potassium-independent <scp>L</scp>-asparaginase from common bean
Unique double-helical packing of protein molecules in the crystal of potassium-independent <span>L</span>-asparaginase from common bean Open
Common bean ( Phaseolus vulgaris ) encodes three class 2 L-asparaginase enzymes: two potassium-dependent enzymes [PvAIII(K)-1 and PvAIII(K)-2] and a potassium-independent enzyme (PvAIII). Here, we present the crystal structure of PvAIII, w…
View article: Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis
Probing the enzymatic activity and maturation process of the EcAIII Ntn-amidohydrolase using local random mutagenesis Open
This report describes a comprehensive approach to local random mutagenesis of the E. coli Ntn-amidohydrolase EcAIII, and supplements the results published earlier for the randomization series RDM1. Here, random mutagenesis was applied in t…