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View article: Presenilin mutations disrupt iron homeostasis to promote ferroptosis mediated neurodegeneration in Caenorhabditis elegans
Presenilin mutations disrupt iron homeostasis to promote ferroptosis mediated neurodegeneration in Caenorhabditis elegans Open
Iron is a vital trace element involved in numerous physiological processes, but it becomes toxic when present in excess. Disruption of iron balance in the brain has been linked to the development of neurodegenerative diseases such as Alzhe…
View article: A detailed protocol for expansion microscopy of <i>Paracentrotus lividus</i> embryos and larvae: Incorporating decalcification for improved imaging
A detailed protocol for expansion microscopy of <i>Paracentrotus lividus</i> embryos and larvae: Incorporating decalcification for improved imaging Open
Expansion microscopy (ExM) enables superresolution imaging by embedding biological specimens in a swellable hydrogel, followed by optical clearing and physical expansion. Here we present a detailed protocol for applying ExM to embryonic st…
View article: Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit
Mapping EGFR1 sorting domains in endosomes with a calibrated 3D expansion microscopy toolkit Open
Endosomes are nanoscale intracellular compartments that sort and recycle cell surface receptors such as epidermal growth factor receptor 1 (EGFR1). Nanometre-scale interactions and co-clustering of signalling proteins, cargo, and the membr…
View article: The evolving landscape of procurement methods in the construction industry: A literature review
The evolving landscape of procurement methods in the construction industry: A literature review Open
The construction industry has acknowledged that novel procurement methods could improve project deliverables quality, reduce costs, and speed up construction. It is vital to examine the available procurement methods for decision-making reg…
View article: Expansion microscopy reveals nano-scale insights into the human neuromuscular junction
Expansion microscopy reveals nano-scale insights into the human neuromuscular junction Open
The neuromuscular junction (NMJ) is a specialized synapse that relays signals from the lower motor neuron to the skeletal muscle. Here, we detail the development and application of expansion microscopy (ExM) as a highly accessible, relativ…
View article: Quantitative sub-cellular imaging with expansion microscopy
Quantitative sub-cellular imaging with expansion microscopy Open
View article: Presenilin mutations disrupt iron homeostasis to promote ferroptosis mediated neurodegeneration in Caenorhabditis elegans
Presenilin mutations disrupt iron homeostasis to promote ferroptosis mediated neurodegeneration in Caenorhabditis elegans Open
View article: Cysteine post‐translational modifications regulate protein interactions of caveolin‐3
Cysteine post‐translational modifications regulate protein interactions of caveolin‐3 Open
Caveolae are small flask‐shaped invaginations of the surface membrane which are proposed to recruit and co‐localize signaling molecules. The distinctive caveolar shape is achieved by the oligomeric structural protein caveolin, of which thr…
View article: Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons
Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons Open
Coordinated events of calcium (Ca2+) released from the endoplasmic reticulum (ER) are key second messengers in excitable cells. In pain-sensing dorsal root ganglion (DRG) neurons, these events can be observed as Ca2+ sparks, produced by a …
View article: Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping
Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping Open
Expansion microscopy (ExM) is a versatile super-resolution microscopy pipeline, leveraging nanoscale biomolecular crosslinking and osmotically driven swelling of hydrogels. Currently, ExM is a laborious and skill-intensive technique, invol…
View article: Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons
Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons Open
This is the data supplement for the paper entitled, "Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons"There are two principal subdirectories within the enclosed zip fil…
View article: Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons
Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons Open
This is the data supplement for the paper entitled, "Super-resolution analysis of the origins of the elementary events of ER calcium release in dorsal root ganglion neurons"There are two principal subdirectories within the enclosed zip fil…
View article: Correction: Expansion microscopy reveals subdomains in<i>C. elegans</i>germ granules
Correction: Expansion microscopy reveals subdomains in<i>C. elegans</i>germ granules Open
Correction to Materials and Methods.
View article: Supplementary data: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping
Supplementary data: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping Open
--- Data supplement--- --- Title: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping The zip file contains the following directories and subdirectories. The files, recommended software, c…
View article: Supplementary data: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping
Supplementary data: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping Open
--- Data supplement--- --- Title: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping The zip file contains the following directories and subdirectories. The files, recommended software, c…
View article: A Systematic review of vertical construction project characteristics
A Systematic review of vertical construction project characteristics Open
The construction industry is one of the key contributors to the development of any country. Depending on the orientation of the construction, it divides into two types; vertical and horizontal constructions. However, different project char…
View article: Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease Open
Rapid release of calcium from internal stores via ryanodine receptors (RyRs) is one of the fastest types of cytoplasmic second messenger signalling in excitable cells. In the heart, rapid summation of the elementary events of calcium relea…
View article: Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy Open
Amine-reactive esters of aromatic fluorescent dyes are emerging as imaging probes for nondescript staining of cellular and tissue architectures. We characterised the differential staining patterns of 14 fluorescent dye ester species with v…
View article: Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping
Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping Open
Expansion microscopy (ExM) is a versatile super-resolution microscopy pipeline, leveraging nanoscale biomolecular cross- linking and osmotically driven swelling of hydrogels. In its current implementation, ExM remains a laborious and skill…
View article: Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease Open
Rapid release of calcium from internal stores via ryanodine receptors (RyRs) is one of the fastest types of cytoplasmic second messenger signalling in excitable cells. In the heart, rapid summation of the elementary events of calcium relea…
View article: Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules
Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules Open
Light and electron microscopy techniques have been indispensable in the identification and characterization of liquid–liquid phase separation membraneless organelles. However, for complex membraneless organelles such as the perinuclear ger…
View article: Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy Open
14 fluorescent NHS esters of different hydrophobicity and charges were shown to differentially label a range of subcellular compartments in HeLa cells using the super-resolution imaging technique expansion microscopy.
View article: Data supplement from Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Data supplement from Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease Open
This file contains correlative calcium and DNA-PAINT datasets acquired from both Ctrl-RV and MCT-RV groups, custom-written analysis code and output files.
View article: Dismantling barriers faced by women in STEM
Dismantling barriers faced by women in STEM Open
View article: Increased SERCA2a sub-cellular heterogeneity in right-ventricular heart failure inhibits excitation-contraction coupling and modulates arrhythmogenic dynamics
Increased SERCA2a sub-cellular heterogeneity in right-ventricular heart failure inhibits excitation-contraction coupling and modulates arrhythmogenic dynamics Open
The intracellular calcium handling system of cardiomyocytes is responsible for controlling excitation-contraction coupling (ECC) and has been linked to pro-arrhythmogenic cellular phenomena in conditions such as heart failure (HF). SERCA2a…
View article: Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads Open
Clusters of ryanodine receptor calcium channels (RyRs) form the primary molecular machinery of intracellular calcium signalling in cardiomyocytes. While a range of optical super-resolution microscopy techniques have revealed the nanoscale …
View article: Cysteine post-translational modifications regulate protein interactions of caveolin-3
Cysteine post-translational modifications regulate protein interactions of caveolin-3 Open
Caveolae are small flask-shaped invaginations of the surface membrane which are proposed to recruit and co-localise signalling molecules. The distinctive caveolar shape is achieved by the oligomeric structural protein caveolin, of which th…
View article: Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules
Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules Open
Many membraneless organelles (MLOs) have been shown to form via liquid-liquid phase separation (LLPS). Light and electron microscopy techniques have been indispensable in the identification and characterization of LLPS MLOs. However, for c…
View article: Supplementary data from Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Supplementary data from Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads Open
Clusters of ryanodine receptor calcium channels (RyRs) form the primary molecular machinery of intracellular calcium signalling in cardiomyocytes. While a range of optical super-resolution microscopy techniques have revealed the nanoscale …
View article: STEM Pride: Perspectives from transgender, nonbinary, and genderqueer scientists
STEM Pride: Perspectives from transgender, nonbinary, and genderqueer scientists Open