Justin L. P. Benesch
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View article: Best practice mass photometry: a guide to optimal single-molecule mass measurement
Best practice mass photometry: a guide to optimal single-molecule mass measurement Open
Mass photometry (MP) has emerged as a powerful approach to study quaternary biomolecular structure, dynamics and interactions. The capabilities of the method ultimately hinge on the ability to accurately measure the tiny optical contrast g…
View article: Single-molecule mass measurements reveal distinct effects of sodium and potassium on mini-spidroin assembly
Single-molecule mass measurements reveal distinct effects of sodium and potassium on mini-spidroin assembly Open
Spider silk formation involves tightly regulated protein assembly influenced by pH and the presence of ions. Kosmotropic salts induce phase separation of spidroins, however, their exact role in assembly is not clear. Here, we investigate h…
View article: Evidence of an activity-enhancing conformational shift in Arabidopsis thaliana plant cysteine oxidase 4 induced by binding of substrate or substrate-mimics
Evidence of an activity-enhancing conformational shift in Arabidopsis thaliana plant cysteine oxidase 4 induced by binding of substrate or substrate-mimics Open
Plant cysteine oxidases (PCOs) are a family of O2-dependent, thiol dioxygenase enzymes that help to coordinate plant responses to flooding by determining the stability of hypoxia-related transcription factors, group VII ethylene…
View article: Uncovering hidden protein modifications with native top-down mass spectrometry
Uncovering hidden protein modifications with native top-down mass spectrometry Open
View article: Light-induced conformational switching and magnetic sensitivity of Drosophila cryptochrome
Light-induced conformational switching and magnetic sensitivity of Drosophila cryptochrome Open
Cryptochromes are light-sensitive flavoproteins with various biological roles, including a proposed function in magnetoreception. This mechanism rests on a magnetically sensitive photochemical reaction of the flavin chromophore with a chai…
View article: Deep learning-based event classification of mass photometry data for optimal mass measurement at the single-molecule level
Deep learning-based event classification of mass photometry data for optimal mass measurement at the single-molecule level Open
Mass photometry (MP) is a powerful technique for studying biomolecular structure, interactions, and dynamics in solution. It detects and quantifies small reflectivity changes at a glass-water interface during protein (un)binding, with sign…
View article: Measurements of molecular size and shape on a chip
Measurements of molecular size and shape on a chip Open
Size and shape are critical discriminators between molecular species and states. We describe a microchip-based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution conditio…
View article: Mass Photometry
Mass Photometry Open
Mass photometry (MP) is a technology for the mass measurement of biological macromolecules in solution. Its mass accuracy and resolution have transformed label-free optical detection into a quantitative measurement, enabling the identifica…
View article: Filamin C dimerisation is regulated by HSPB7
Filamin C dimerisation is regulated by HSPB7 Open
View article: Following phospholipid transfer through the OmpF <sub>3</sub> –MlaA–MlaC lipid shuttle with native mass spectrometry
Following phospholipid transfer through the OmpF <sub>3</sub> –MlaA–MlaC lipid shuttle with native mass spectrometry Open
The maintenance of lipid asymmetry (Mla) system in gram-negative bacteria transfers phospholipids between the outer and inner membrane to maintain the outer membrane asymmetry. Misplaced phospholipids are extracted from the outer leaflet o…
View article: Light-induced conformational switching and magnetic sensitivity of Drosophila cryptochrome
Light-induced conformational switching and magnetic sensitivity of Drosophila cryptochrome Open
SUMMARY Cryptochromes are flavoproteins with a number of established and proposed biological functions based on their sensitivity to light. Amongst the latter is the possibility that cryptochromes mediate the geomagnetic compass sense used…
View article: Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition
Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition Open
Cellular processes are controlled by the thermodynamics of the underlying biomolecular interactions. Frequently, structural investigations use one monomeric binding partner, while ensemble measurements of binding affinities generally yield…
View article: Quantifying the heterogeneity of macromolecular machines by mass photometry
Quantifying the heterogeneity of macromolecular machines by mass photometry Open
View article: Author response: Rapid, DNA-induced interface swapping by DNA gyrase
Author response: Rapid, DNA-induced interface swapping by DNA gyrase Open
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. DNA gyrase can loop DNA around the C-terminal domains (CTDs) of GyrA…
View article: Emergence of fractal geometries in the evolution of a metabolic enzyme
Emergence of fractal geometries in the evolution of a metabolic enzyme Open
View article: Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar
Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar Open
Native mass spectrometry (MS) has become widely accepted in structural biology, providing information on stoichiometry, interactions, homogeneity, and shape of protein complexes. Yet, the fundamental assumption that proteins inside the mas…
View article: Rapid, DNA-induced interface swapping by DNA gyrase
Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: Author Response: Rapid, DNA-induced interface swapping by DNA gyrase
Author Response: Rapid, DNA-induced interface swapping by DNA gyrase Open
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex. GyrA is usually found as a dimer in solution, whereas GyrB can exist…
View article: Regulation of inositol 5-phosphatase activity by the C2 domain of SHIP1 and SHIP2
Regulation of inositol 5-phosphatase activity by the C2 domain of SHIP1 and SHIP2 Open
SHIP1, an inositol 5-phosphatase, plays a central role in cellular signaling. As such, it has been implicated in many conditions. Exploiting SHIP1 as a drug target will require structural knowledge and the design of selective small molecul…
View article: From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y
From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y Open
View article: Cardiac stress leads to regulation of Filamin C dimerisation via an ancient phosphorylation-modulated interaction with HSPB7
Cardiac stress leads to regulation of Filamin C dimerisation via an ancient phosphorylation-modulated interaction with HSPB7 Open
The biomechanical properties and responses of tissues underpin a variety of physiological functions and pathologies. In striated muscle, the actin-binding protein filamin C (FLNC) is a key protein whose variants causative for a wide range …
View article: The adaptability of the ion-binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF
The adaptability of the ion-binding site by the Ag(I)/Cu(I) periplasmic chaperone SilF Open
View article: Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS
Development of a PNGase Rc Column for Online Deglycosylation of Complex Glycoproteins during HDX-MS Open
Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins, and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by diff…
View article: Cooperativity and induced oligomerisation control the interaction of SARS-CoV-2 with its cellular receptor and patient-derived antibodies
Cooperativity and induced oligomerisation control the interaction of SARS-CoV-2 with its cellular receptor and patient-derived antibodies Open
Viral entry is mediated by oligomeric proteins on the virus and cell surfaces. The association is therefore open to multivalent interactions between these proteins, yet such recognition is typically rationalised as affinity between monomer…
View article: Cryo-EM of soft-landed<i>β</i>-galactosidase: Gas-phase and native structures are remarkably similar
Cryo-EM of soft-landed<i>β</i>-galactosidase: Gas-phase and native structures are remarkably similar Open
Native mass spectrometry (native MS) is a powerful technique that provides information on stoichiometry, interactions, homogeneity and shape of protein complexes. However, the extent of deviation between protein structures in the mass spec…
View article: Regulation of inositol 5-phosphatase activity by the C2 domain of SHIP1 and SHIP2
Regulation of inositol 5-phosphatase activity by the C2 domain of SHIP1 and SHIP2 Open
SHIP1, an inositol 5-phosphatase, plays a central role in cellular signalling. As such, it has been implicated in many conditions. Exploiting SHIP1 as a drug target will require structural knowledge and the design of selective small molecu…
View article: Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS
Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS Open
Protein glycosylation is one of the most common PTMs and many cell surface receptors, extracellular proteins and biopharmaceuticals are glycosylated. However, HDX-MS analysis of such important glycoproteins has so far been limited by diffi…
View article: The beauty and complexity of the small heat shock proteins: a report on the proceedings of the fourth workshop on small heat shock proteins
The beauty and complexity of the small heat shock proteins: a report on the proceedings of the fourth workshop on small heat shock proteins Open
The Fourth Cell Stress Society International workshop on small heat shock proteins (sHSPs), a follow-up to successful workshops held in 2014, 2016 and 2018, took place as a virtual meeting on the 17-18 November 2022. The meeting was design…
View article: Dimerization of European Robin Cryptochrome 4a
Dimerization of European Robin Cryptochrome 4a Open
Homo-dimer formation is important for the function of many proteins. Although dimeric forms of cryptochromes (Cry) have been found by crystallography and were recently observed in vitro for European robin Cry4a, little is known about the d…