Paul Garside
YOU?
Author Swipe
View article: The effect of abatacept on T-cell activation is not long-lived <i>in vivo</i>
The effect of abatacept on T-cell activation is not long-lived <i>in vivo</i> Open
Abatacept, a co-stimulatory blocker comprising the extracellular portion of human CTLA-4 linked to the Fc region of IgG1, is approved for the treatment of rheumatoid arthritis. By impairing the interaction between CD28 on T cells and CD80/…
View article: A discovery pipeline for identification and <i>in vivo</i> validation of drugs that alter T cell/ dendritic cell interaction
A discovery pipeline for identification and <i>in vivo</i> validation of drugs that alter T cell/ dendritic cell interaction Open
The interaction between T cells and dendritic cells (DC) is central to determining whether or not an adaptive immune response develops. Autoimmune diseases like rheumatoid arthritis result from inappropriate activation of self-antigen spec…
View article: Dissecting the molecular control of immune cell accumulation in the inflamed joint
Dissecting the molecular control of immune cell accumulation in the inflamed joint Open
Mechanisms governing entry and exit of immune cells into and out of inflamed joints remain poorly understood. We sought herein to identify the key molecular pathways regulating such migration. Using murine models of inflammation in conjunc…
View article: Orally administered antigen can reduce or exacerbate pathology in an animal model of inflammatory arthritis dependent upon the timing of administration
Orally administered antigen can reduce or exacerbate pathology in an animal model of inflammatory arthritis dependent upon the timing of administration Open
Currently, treatments for rheumatoid arthritis (RA) are focussed on treatment of disease symptoms rather than addressing the cause of disease, which could lead to remission and cure. Central to disease development is the induction of autoi…
View article: Revealing stromal and lymphoid sources of <i>Col3a1</i>-expression during inflammation using a novel reporter mouse
Revealing stromal and lymphoid sources of <i>Col3a1</i>-expression during inflammation using a novel reporter mouse Open
One of the earliest signs of dysregulation of the homeostatic process of fibrosis, associated with pathology in chronic conditions such as rheumatoid arthritis, is the overexpression of collagen type III (COL-3). Critically, there is still…
View article: Orally administered antigen can reduce or exacerbate pathology in an animal model of inflammatory arthritis dependent upon the timing of administration
Orally administered antigen can reduce or exacerbate pathology in an animal model of inflammatory arthritis dependent upon the timing of administration Open
Currently, treatments for rheumatoid arthritis (RA) are focussed on management of disease symptoms rather than addressing the cause of disease, which could lead to remission and cure. Central to disease development is the induction of auto…
View article: Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis
Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis Open
Human African Trypanosomiasis (HAT) has been responsible for several deadly epidemics throughout the 20th century, but a renewed commitment to disease control has significantly reduced new cases and motivated a target for the elimination o…
View article: Corrigendum: To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host
Corrigendum: To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host Open
[This corrects the article DOI: 10.3389/fimmu.2020.01250.].
View article: Preclinical models of arthritis for studying immunotherapy and immune tolerance
Preclinical models of arthritis for studying immunotherapy and immune tolerance Open
Increasingly earlier identification of individuals at high risk of rheumatoid arthritis (RA) (eg, with autoantibodies and mild symptoms) improves the feasibility of preventing or curing disease. The use of antigen-specific immunotherapies …
View article: Nanoalum adjuvanted vaccines: small details make a big difference
Nanoalum adjuvanted vaccines: small details make a big difference Open
Purified vaccine antigens offer important safety and reactogenicity advantages compared with live attenuated or whole killed virus and bacterial vaccines. However, they require the addition of adjuvants to induce the magnitude, duration an…
View article: TCRβ Sequencing Reveals Spatial and Temporal Evolution of Clonal CD4 T Cell Responses in a Breach of Tolerance Model of Inflammatory Arthritis
TCRβ Sequencing Reveals Spatial and Temporal Evolution of Clonal CD4 T Cell Responses in a Breach of Tolerance Model of Inflammatory Arthritis Open
Effective tolerogenic intervention in Rheumatoid Arthritis (RA) will rely upon understanding the evolution of articular antigen specific CD4 T cell responses. TCR clonality of endogenous CD4 T cell infiltrates in early inflammatory arthrit…
View article: Targeting Opposing Immunological Roles of the Junctional Adhesion Molecule-A in Autoimmunity and Cancer
Targeting Opposing Immunological Roles of the Junctional Adhesion Molecule-A in Autoimmunity and Cancer Open
The junctional adhesion molecule-A (JAM-A) is a cell surface adhesion molecule expressed on platelets, epithelial cells, endothelial cells and leukocytes (e. g. monocytes and dendritic cells). JAM-A plays a relevant role in leukocyte traff…
View article: TCRβ Sequencing Reveals Spatial and Temporal Evolution of Clonal CD4 T cell Responses in a Breach of Tolerance Model of Inflammatory Arthritis
TCRβ Sequencing Reveals Spatial and Temporal Evolution of Clonal CD4 T cell Responses in a Breach of Tolerance Model of Inflammatory Arthritis Open
Effective tolerogenic intervention in Rheumatoid Arthritis (RA) will rely upon understanding the evolution of articular antigen specific CD4 T cell responses. TCR clonality of endogenous CD4 T cell infiltrates in early inflammatory arthrit…
View article: Tolerance induction in memory CD4 T cells is partial and reversible
Tolerance induction in memory CD4 T cells is partial and reversible Open
Summary Memory T cells respond rapidly in part because they are less reliant on a heightened levels of costimulatory molecules. This enables rapid control of secondary infecting pathogens but presents challenges to efforts to control or si…
View article: The Impact of Malaria Parasites on Dendritic Cell–T Cell Interaction
The Impact of Malaria Parasites on Dendritic Cell–T Cell Interaction Open
Malaria is caused by apicomplexan parasites of the genus Plasmodium. While infection continues to pose a risk for the majority of the global population, the burden of disease mainly resides in Sub-Saharan Africa. Although immunity develops…
View article: To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host
To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host Open
African trypanosomes are single-celled extracellular protozoan parasites transmitted by tsetse fly vectors across sub-Saharan Africa, causing serious disease in both humans and animals. Mammalian infections begin when the tsetse fly penetr…
View article: Tolerance induction in memory CD4 T cells is partial and reversible
Tolerance induction in memory CD4 T cells is partial and reversible Open
Memory T cells respond rapidly in part because they are less reliant on heightened levels of costimulatory molecules. This presents challenges to silencing memory T cells in tolerance strategies for autoimmunity or allergy. We find that me…
View article: Developing a xenograft model of human vasculature in the mouse ear pinna
Developing a xenograft model of human vasculature in the mouse ear pinna Open
Humanised xenograft models allow for the analysis of human tissue within a physiological environment in vivo . However, current models often rely on the angiogenesis and ingrowth of recipient vasculature to perfuse tissues, preventing anal…
View article: Developing a xenograft model of human vasculature in the mouse ear pinna
Developing a xenograft model of human vasculature in the mouse ear pinna Open
Humanised xenograft models allow for the analysis of human tissue within a physiological environment in vivo . However, current models often rely on the angiogenesis and ingrowth of recipient vasculature to perfuse tissues, preventing anal…
View article: A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis
A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis Open
Atherosclerosis is a complex inflammatory pathology underpinning cardiovascular diseases (CVD), which are the leading cause of death worldwide. The interplay between vascular stromal cells and immune cells is fundamental to the progression…
View article: The aorta can act as a site of naïve CD4+ T-cell priming
The aorta can act as a site of naïve CD4+ T-cell priming Open
Our results demonstrate that the aorta can support T-cell priming and that naïve T cells traffic between the circulation and vessel wall. These data underpin the paradigm that local priming of T cells specific for plaque antigens contribut…
View article: Spatiotemporal Modeling of the Key Migratory Events During the Initiation of Adaptive Immunity
Spatiotemporal Modeling of the Key Migratory Events During the Initiation of Adaptive Immunity Open
Initiation of adaptive immunity involves distinct migratory cell populations coming together in a highly dynamic and spatially organized process. However, we lack a detailed spatiotemporal map of these events due to our inability to track …
View article: Resolving the apparent transmission paradox of African sleeping sickness
Resolving the apparent transmission paradox of African sleeping sickness Open
Human African trypanosomiasis (HAT), or African sleeping sickness, is a fatal disease found throughout sub-Saharan Africa. The disease is close to elimination in many areas, although it was similarly close to elimination once before and su…
View article: Effects of host‐derived chemokines on the motility and viability of <i>Trypanosoma brucei</i>
Effects of host‐derived chemokines on the motility and viability of <i>Trypanosoma brucei</i> Open
Summary African trypanosomes ( Trypanosoma brucei spp.) are extracellular, hemoflagellate, protozoan parasites. Mammalian infection begins when the tsetse fly vector injects trypanosomes into the skin during blood feeding. The trypanosomes…
View article: A Novel Cellular Pathway of Antigen Presentation and CD4 T Cell Activation in vivo
A Novel Cellular Pathway of Antigen Presentation and CD4 T Cell Activation in vivo Open
Dendritic cell activation of CD4 T cells in the lymph node draining a site of infection or vaccination is widely considered the central event in initiating adaptive immunity. The accepted dogma is that this occurs by stimulating local acti…
View article: Non-Invasive Multiphoton Imaging of Islets Transplanted Into the Pinna of the NOD Mouse Ear Reveals the Immediate Effect of Anti-CD3 Treatment in Autoimmune Diabetes
Non-Invasive Multiphoton Imaging of Islets Transplanted Into the Pinna of the NOD Mouse Ear Reveals the Immediate Effect of Anti-CD3 Treatment in Autoimmune Diabetes Open
We present a novel and readily accessible method facilitating cellular time-resolved imaging of transplanted pancreatic islets. Grafting of islets to the mouse ear pinna allows non-invasive, in vivo longitudinal imaging of events in the is…