Joachim Schulz
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View article: Standard Sample Preparation for Serial Femtosecond Crystallography
Standard Sample Preparation for Serial Femtosecond Crystallography Open
The development of serial crystallography (SX), including serial synchrotron crystallography (SSX) at synchrotron sources and serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs), has facilitated the collection of…
View article: Pr and Pfr structures of plant phytochrome A
Pr and Pfr structures of plant phytochrome A Open
Phytochromes are biliprotein photoreceptors widespread amongst microorganisms and ubiquitous in plants where they control developmental processes as diverse as germination, stem elongation and floral induction through the photoconversion o…
View article: Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers
Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers Open
Single-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature. A coaxial liquid-sheet strategy considerably extended the range of …
View article: Crystal optics select weak response of ultrafast crystallography of the Photoactive Yellow Protein
Crystal optics select weak response of ultrafast crystallography of the Photoactive Yellow Protein Open
Ultrafast crystallography of light-induced processes can directly capture structural differences driven by short-pulse laser illumination. With short pump-probe observation time and short laser pulse durations, the electronic and nuclear d…
View article: A liquid jet sample environment for experiments on liquids at MID (EuXFEL)
A liquid jet sample environment for experiments on liquids at MID (EuXFEL) Open
An experimental setup for liquid jet applications was implemented at the Materials Imaging and Dynamics Instrument (MID) of the European XFEL. This setup is operated in the multi-purpose experimental chamber of MID at pressures down to 10 …
View article: Femtosecond pulse pair distribution functions at MID at the European XFEL
Femtosecond pulse pair distribution functions at MID at the European XFEL Open
Pair distribution functions from individual femtosecond X-ray pulses have the potential to elucidate the structure of transient states in matter, such as those found in liquid systems. To demonstrate this possibility an experiment was cond…
View article: Ambient temperature structural studies of Penicillin-binding Protein 2a of<i>Methicillin-Resistant Staphylococcus aureus</i>with XFELs and synchrotrons
Ambient temperature structural studies of Penicillin-binding Protein 2a of<i>Methicillin-Resistant Staphylococcus aureus</i>with XFELs and synchrotrons Open
Penicillin-binding protein 2a (PBP2a) is a transpeptidase responsible for the β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA), posing significant challenges to antibiotic therapy. PBP2a’s unique structural feature…
View article: High Incidence and Impact of Suspected Exocrine Pancreatic Insufficiency in Patients Post‐Hematopoietic Stem Cell Transplantation: A Single‐Center Prospective Observational Study
High Incidence and Impact of Suspected Exocrine Pancreatic Insufficiency in Patients Post‐Hematopoietic Stem Cell Transplantation: A Single‐Center Prospective Observational Study Open
Exocrine pancreatic insufficiency (EPI) is suspected but remains understudied in immunosuppressed conditions such as post‐hematopoietic stem cell transplantation (HSCT). This prospective observational study aimed to investigate the inciden…
View article: Ophthalmoplegia and vision loss in extrapulmonary tuberculosis with bilateral cavernous sinus involvement
Ophthalmoplegia and vision loss in extrapulmonary tuberculosis with bilateral cavernous sinus involvement Open
View article: Pr and Pfr structures of plant phytochrome A
Pr and Pfr structures of plant phytochrome A Open
Phytochromes are biliprotein photoreceptors widespread amongst microorganisms and ubiquitous in plants where they control developmental processes as diverse as germination, stem elongation and floral induction through the photoconversion o…
View article: New insights into the function and molecular mechanisms of Ferredoxin-NADP+ reductase from Brucella ovis
New insights into the function and molecular mechanisms of Ferredoxin-NADP+ reductase from Brucella ovis Open
Bacterial ferredoxin(flavodoxin)-NADP+ reductases (FPR) primarily catalyze the transfer of reducing equivalents from NADPH to ferredoxin (or flavodoxin) to provide low potential reducing equivalents for the oxidoreductive metabo…
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography Open
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the …
View article: Development of a flat jet delivery system for soft X-ray spectroscopy at MAX IV
Development of a flat jet delivery system for soft X-ray spectroscopy at MAX IV Open
One of the most challenging aspects of X-ray research is the delivery of liquid sample flows into the soft X-ray beam. Currently, cylindrical microjets are the most commonly used sample injection systems for soft X-ray liquid spectroscopy.…
View article: G2019S Mutation of Leucine-Rich Repeat Kinase 2 Is a Cause of Lewy Body Dementia in Patients With North African Ancestors
G2019S Mutation of Leucine-Rich Repeat Kinase 2 Is a Cause of Lewy Body Dementia in Patients With North African Ancestors Open
Background: Mutations in the LRRK2 gene are the most common genetic cause of Parkinson disease but are believed to play no significant role in Lewy body disease (LBD). Objectives: As the frequency of G2019S LRRK2 mutation is extremely high…
View article: Aqueous Ammonium Nitrate Investigated Using Photoelectron Spectroscopy of Cylindrical and Flat Liquid Jets
Aqueous Ammonium Nitrate Investigated Using Photoelectron Spectroscopy of Cylindrical and Flat Liquid Jets Open
Ammonium nitrate in aqueous solution was investigated with synchrotron radiation based photoelectron spectroscopy using two types of liquid jet nozzles. Electron emission from a cylindrical microjet of aqueous ammonium nitrate solution was…
View article: SARS-CoV-2 Mpro responds to oxidation by forming disulfide and NOS/SONOS bonds
SARS-CoV-2 Mpro responds to oxidation by forming disulfide and NOS/SONOS bonds Open
View article: Minimized Sample Consumption for Time-Resolved Serial Crystallography Applied to the Redox Cycle of Human NQO1
Minimized Sample Consumption for Time-Resolved Serial Crystallography Applied to the Redox Cycle of Human NQO1 Open
Sample consumption for serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) remains a major limitation preventing broader use of this powerful technology in macromolecular crystallography. This drawback is exace…
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography Open
OaPAC is a recently discovered b lue-light u sing flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into th…
View article: Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE
Kilohertz droplet-on-demand serial femtosecond crystallography at the European XFEL station FXE Open
X-ray Free Electron Lasers (XFELs) allow the collection of high-quality serial femtosecond crystallography data. The next generation of megahertz superconducting FELs promises to drastically reduce data collection times, enabling the captu…
View article: Convolutional neural network approach for the automated identification of <i>in cellulo</i> crystals
Convolutional neural network approach for the automated identification of <i>in cellulo</i> crystals Open
In cellulo crystallization is a rare event in nature. Recent advances that have made use of heterologous overexpression can promote the intracellular formation of protein crystals, but new tools are required to detect and characterize thes…
View article: Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments
Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments Open
Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated …
View article: Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack
Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack Open
This work investigates the performance of the electrospray aerosol generator at the European X-ray Free Electron Laser (EuXFEL). This generator is, together with an aerodynamic lens stack that transports the particles into the X-ray intera…
View article: Implementing dark-field contrast imaging in computer tomography with simple setup Talbot-Lau-Interferometer
Implementing dark-field contrast imaging in computer tomography with simple setup Talbot-Lau-Interferometer Open
View article: Light-induced Trpin/Metout Switching During BLUF Domain Activation in ATP-bound Photoactivatable Adenylate Cyclase OaPAC
Light-induced Trpin/Metout Switching During BLUF Domain Activation in ATP-bound Photoactivatable Adenylate Cyclase OaPAC Open
The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and …
View article: Experimental capabilities for liquid jet samples at sub-MHz rates at the FXE Instrument at European XFEL
Experimental capabilities for liquid jet samples at sub-MHz rates at the FXE Instrument at European XFEL Open
The Femtosecond X-ray Experiments (FXE) instrument at the European X-ray Free-Electron Laser (EuXFEL) provides an optimized platform for investigations of ultrafast physical, chemical and biological processes. It operates in the energy ran…
View article: Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS)
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS) Open
Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules are not a…
View article: Helium-Electrospray: an improved sample delivery system for single-particle imaging with X-ray lasers
Helium-Electrospray: an improved sample delivery system for single-particle imaging with X-ray lasers Open
Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated …
View article: Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC
Light-induced Trpin/Metout switching during BLUF domain activation in ATP-bound photoactivatable adenylate cyclase OaPAC Open
The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and …
View article: Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography
Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography Open
Time-resolved crystallography enables the visualization of protein molecular motion during a reaction. Although light is often used to initiate reactions in time-resolved crystallography, only a small number of proteins can be activated by…
View article: Convolutional neural network approach for the automated identification of<i>in cellulo</i>crystals
Convolutional neural network approach for the automated identification of<i>in cellulo</i>crystals Open
In cellulo crystallization is a rarely occurring event in nature. Recent advances, making use of heterologous overexpression, can promote the intracellular formation of protein crystals, but new tools are required to detect and to characte…