Hiren J. Joshi
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View article: Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins
Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins Open
The mucus lining wet body surfaces forms the interphase and barrier for the microbiota and resident microbiomes. Large mucin proteins densely decorated with O-glycans make up the mucus lining to entrap, feed and shape the microbiota, and r…
View article: Antithrombin-binding heparan sulfate is ubiquitously expressed in epithelial cells and suppresses pancreatic tumorigenesis
Antithrombin-binding heparan sulfate is ubiquitously expressed in epithelial cells and suppresses pancreatic tumorigenesis Open
3-O-sulfation of heparan sulfate (HS) is the key determinant for binding and activation of antithrombin III (AT). This interaction is the basis of heparin treatment to prevent thrombotic events and excess coagulation. Antithrombin-binding …
View article: O-mannosylation and protein maturation check-points represent therapeutic opportunities in BRAF fusion protein oncogenesis
O-mannosylation and protein maturation check-points represent therapeutic opportunities in BRAF fusion protein oncogenesis Open
Fusions between protein-coding genes are common oncogenic drivers across cancers, typically pairing a proto-oncogene with partner that does not independently drive cancer. In all therapeutically actionable fusions, the proto-oncogene is th…
View article: Map of the neuronal O-glycoproteome reveals driver functions in the regulated secretory pathway
Map of the neuronal O-glycoproteome reveals driver functions in the regulated secretory pathway Open
Impairments in protein glycosylation, including O-GalNAc-type glycosylation, are linked to severe developmental disorders with prominent neurological involvement. However, the role of this glycosylation pathway at a cellular level is not y…
View article: Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics Open
View article: Mammalian cell-based production of glycans, glycopeptides and glycomodules
Mammalian cell-based production of glycans, glycopeptides and glycomodules Open
View article: Targeting host O-linked glycan biosynthesis affects Ebola virus replication efficiency and reveals differential GalNAc-T acceptor site preferences on the Ebola virus glycoprotein
Targeting host O-linked glycan biosynthesis affects Ebola virus replication efficiency and reveals differential GalNAc-T acceptor site preferences on the Ebola virus glycoprotein Open
Ebola virus glycoprotein (EBOV GP) is one of the most heavily O-glycosylated viral glycoproteins, yet we still lack a fundamental understanding of the structure of its large O-glycosylated mucin-like domain and to what degree the host O-gl…
View article: Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall
Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall Open
Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to …
View article: Functional implications of glycans and their curation: insights from the workshop held at the 16th Annual International Biocuration Conference in Padua, Italy
Functional implications of glycans and their curation: insights from the workshop held at the 16th Annual International Biocuration Conference in Padua, Italy Open
Dynamic changes in protein glycosylation impact human health and disease progression. However, current resources that capture disease and phenotype information focus primarily on the macromolecules within the central dogma of molecular bio…
View article: High-throughput and scalable single cell proteomics identifies over 5000 proteins per cell
High-throughput and scalable single cell proteomics identifies over 5000 proteins per cell Open
The emergence of mass spectrometry (MS)-based single-cell proteomics (SCP) promise to revolutionize the study of cellular biology and biomedicine by providing an unparalleled view of the proteome in individual cells. Despite its groundbrea…
View article: Dataset for "A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases"
Dataset for "A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases" Open
This dataset provides the classical and QM/MM MD simulation trajectory data to the manuscript: A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases The data set contains classical MD simulations of…
View article: Dataset for "A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases"
Dataset for "A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases" Open
This dataset provides the classical and QM/MM MD simulation trajectory data to the manuscript: A Family of Di-Glutamate Mucin Degrading Enzymes that Bridges Glycan Hydrolases and Peptidases The data set contains classical MD simulations of…
View article: The SHDRA syndrome-associated gene <i>TMEM260</i> encodes a protein-specific O-mannosyltransferase
The SHDRA syndrome-associated gene <i>TMEM260</i> encodes a protein-specific O-mannosyltransferase Open
Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome, but the function of the encoded protein remains unknown. We previously reported wide occurrence of O-mannose glycans on extracellular immunoglobulin…
View article: Tailoring Biocontrol: Selective Enrichment of Stress Tolerant Bacteriophages for Effective Pseudomonas Aeruginosa Contamination Management in Food
Tailoring Biocontrol: Selective Enrichment of Stress Tolerant Bacteriophages for Effective Pseudomonas Aeruginosa Contamination Management in Food Open
View article: Applying transcriptomics to study glycosylation at the cell type level
Applying transcriptomics to study glycosylation at the cell type level Open
View article: The SHDRA syndrome associated gene TMEM260 encodes a protein-specific O-mannosyltransferase
The SHDRA syndrome associated gene TMEM260 encodes a protein-specific O-mannosyltransferase Open
Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome (SHDRA), but the function of the encoded protein remains unknown. We report that TMEM260 is an ER-located protein O-mannosyltransferase that selectiv…
View article: Display of the human mucinome with defined O-glycans by gene engineered cells
Display of the human mucinome with defined O-glycans by gene engineered cells Open
View article: An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles
An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles Open
View article: Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome
Cell-Based Glycan Arrays—A Practical Guide to Dissect the Human Glycome Open
View article: O‐glycan initiation directs distinct biological pathways and controls epithelial differentiation
O‐glycan initiation directs distinct biological pathways and controls epithelial differentiation Open
View article: Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody
Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody Open
The molecular basis of an anti-MUC1 antibody that recognizes the entire GalNAc unit as a primary epitope is disclosed.
View article: An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells Open
View article: Exploring Regulation of Protein O-Glycosylation in Isogenic Human HEK293 Cells by Differential O-Glycoproteomics
Exploring Regulation of Protein O-Glycosylation in Isogenic Human HEK293 Cells by Differential O-Glycoproteomics Open
Most proteins trafficking the secretory pathway of metazoan cells will acquire GalNAc-type O-glycosylation. GalNAc-type O-glycosylation is differentially regulated in cells by the expression of a repertoire of up to twenty genes encoding p…
View article: A strategy for generating cancer-specific monoclonal antibodies to aberrant<i>O</i>-glycoproteins: identification of a novel dysadherin-Tn antibody
A strategy for generating cancer-specific monoclonal antibodies to aberrant<i>O</i>-glycoproteins: identification of a novel dysadherin-Tn antibody Open
Successful application of potent antibody-based T-cell engaging immunotherapeutic strategies is currently limited mainly to hematological cancers. One major reason is the lack of well-characterized antigens on solid tumors with sufficient …
View article: Program and Abstracts for 2018 Annual Meeting of the Society for Glycobiology
Program and Abstracts for 2018 Annual Meeting of the Society for Glycobiology Open
Protein glycosylation is fundamentally important to most biological processes and it is often important to regulate it in biopharmaceutical development. Thus, substantial efforts have been taken to engineer glycosylation of a variety of bi…
View article: Probing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines
Probing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines Open
The GalNAc-type O-glycoproteome is orchestrated by a large family of polypeptide GalNAc-transferase isoenzymes (GalNAc-Ts) with partially overlapping contributions to the O-glycoproteome besides distinct nonredundant function…
View article: Viral glycoproteomes: technologies for characterization and outlook for vaccine design
Viral glycoproteomes: technologies for characterization and outlook for vaccine design Open
It has long been known that surface proteins of most enveloped viruses are covered with glycans. It has furthermore been demonstrated that glycosylation is essential for propagation and immune evasion for many viruses. The recent developme…
View article: TAILS N-terminomics and proteomics reveal complex regulation of proteolytic cleavage by O-glycosylation
TAILS N-terminomics and proteomics reveal complex regulation of proteolytic cleavage by O-glycosylation Open
Proteolytic processing is an irreversible post-translational modification functioning as a ubiquitous regulator of cellular activity. Protease activity is tightly regulated via control of gene expression, enzyme and substrate compartmental…
View article: Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases
Glycosyltransferase genes that cause monogenic congenital disorders of glycosylation are distinct from glycosyltransferase genes associated with complex diseases Open
Glycosylation of proteins, lipids and proteoglycans in human cells involves at least 167 identified glycosyltransferases (GTfs), and these orchestrate the biosynthesis of diverse types of glycoconjugates and glycan structures. Mutations in…
View article: The cellular impact of glycoengineering
The cellular impact of glycoengineering Open