Explanipedia

Cloning and dependence pattern of the sporulation operon spoVH Open

Simon M. Cutting, J. Mandelstam · 1988

The spoVH locus, involved in the sporulation of Bacillus subtilis, was cloned in derivatives of the temperate bacteriophage luminal diameter 105. Two recombinant phages were obtained which contained 4.2 kilobases of chromosomal DNA. Both p…

Branched pattern of regulatory interactions between late sporulation genes in Bacillus subtilis Open

Jeff Errington, Simon M. Cutting, J. Mandelstam · 1988

We measured the synthesis of dipicolinic acid (DPA) during sporulation in spo mutants of Bacillus subtilis by a sensitive biological assay based on cross-feeding of a spoVF mutant strain and also chemically. Many spo mutations, including s…

Use of a lacZ Gene Fusion to Determine the Dependence Pattern and the Spore Compartment Expression of Sporulation Operon spoVA in spo Mutants of Bacillus subtilis Open

Jeff Errington, J. Mandelstam · 1986

A spoVAA::lacZ gene fusion has been used to study expression of the spoVA operon during sporulation in Bacillus subtilis. beta-Galactosidase activity, encoded by the fusion gene, begins to be produced about 2.5 h after the induction of spo…

The Nucleotide Sequence and the Transcription During Sporulation of the gerE Gene of Bacillus subtilis Open

Simon M. Cutting, J. Mandelstam · 1986

We have determined the nucleotide sequence of a 1496 bp stretch of Bacillus subtilis chromosome that complements the gerE36 mutation. The sequence contains three open reading frames. One of these is part of the sdhC gene, the other two, wh…

Duplicated sporulation genes in bacteria Open

Jeff Errington, Philippe Fort, J. Mandelstam · 1985

Statistical analysis indicates that about 150 genes might be needed for spore formation in Bacillus subtilis [1]. We have sequenced 11 of these and examined the predicted amino acid sequences for the possibility that present‐day sporulatin…

Identification of a New Sporulation Locus, spoIIIF, in Bacillus subtilis Open

Iain L. Lamont, J. Mandelstam · 1984

We have isolated a mutant of Bacillus subtilis, strain 590, which is blocked at stage III of sporulation. The spo mutation which is carried by this strain is linked to pheA by transformation and defines a previously unidentified locus, spo…

Cloning of the Bacillus subtilis lys and spoIIIB Genes in Phage 105 Open

Howard F. Jenkinson, J. Mandelstam · 1983

The lys gene of Bacillus subtilis was inserted into prophage phi 105. The recombinant phage (phi 105dlys) contained DNA which was about 2 MDal smaller than the wild-type phage DNA, and the phage particles had no tails. The phage did not pl…

Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation Open

Patrick Dion, J. Mandelstam · 1980

At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; …

Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them Open

Howard F. Jenkinson, D. Kay, J. Mandelstam · 1980

During sporulation in replacement medium, resistance to toluene to heating at 65 degrees C, to lysozyme, and to heating at 80 degrees C appeared in sequence between 4 and 8 h after the induction of sporulation (i.e., between t4 and t8). Th…

Extracellular manganese-stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis Open

A. Akrigg, J. Mandelstam · 1978

A considerable amount of Mn2+-stimulated DNAase (deoxyribonuclease) activity is released by Bacillus subtilis 168 during sporulation in a glucose-deficient medium; much smaller amounts are released during starvation for phosphate or nitrog…

Resporulation of outgrowing Bacillus subtilis spores Open

A. Keynan, ADRIAN A. BERNS, Gary R. Dunn, Marian F. Young, J. Mandelstam · 1976

Germinated spores of Bacillus subtilis were incubated in outgrowth medium and tested periodically for capacity to sporulate when suspended in sporulation medium. Concurrent measurements were made of deoxyribonucleic acid (DNA) content and …

Order of expression of genes affecting septum location during sporulation of Bacillus subtilis Open

Gary R. Dunn, Dawn Torgersen, J. Mandelstam · 1976

The mean volumes of stationary-phase cells of wild-type and asporogenous mutants of Bacillus subtilis have been measured. Mutants blocked at stage 0 of sporulation either produced cells that had the same volume as the developing sporangium…

Production and possible function of serine protease during sporulation of Bacillus subtilis Open

Brian N. Dancer, J. Mandelstam · 1975

The production of extracellular protease during sporulation in Bacillus subtilis 168 was investigated. Two proteases are produced, an alkaline serine protease and a neutral metalloprotease. In vivo inhibition of the serine protease with ph…

Criteria for categorizing early biochemical events occurring during sporulation of Bacillus subtilis Open

Brian N. Dancer, J. Mandelstam · 1975

Two criteria are suggested for assessing the relevance of biochemical events occurring early in sporulation. The first is thymidine starvation, a condition known to inhibit sporulation. This also inhibits the production of metalloprotease,…

Induction of Sporulation During Synchronized Chromosome Replication in Bacillus subtilis Open

J. Mandelstam, Sonia A. Higgs · 1974

Synchronous chromosome replication was obtained in a culture of Bacillus subtilis temperature-sensitive mutants growing in a rich medium. At intervals during this replication, samples of cells were transferred to a poor medium to induce sp…

Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporulation Open

Daslav Hranueli, P. J. Piggot, J. Mandelstam · 1974

As an alternative to exhaustive mapping, an attempt has been made to obtain a rough estimate of the total number of sporulation operons by statistical treatment. Sixteen sporulation mutants taken at random were characterized biochemically …

Lethal effect of rifampicin in Bacillus subtilis as a complicating factor in the assessment of the lifetime of messenger ribonucleic acid Open

J. G. Coote, David A. Wood, J. Mandelstam · 1973

The bactericidal action of rifampicin was compared with that of chloramphenicol in growing and in sporulating cultures of Bacillus subtilis 168. Chloramphenicol kills cells only very slowly, but exposure to rifampicin kills over 95% of cel…

Use of Constructed Double Mutants for Determining the Temporal Order of Expression of Sporulation Genes in Bacillus subtilis Open

J. G. Coote, J. Mandelstam · 1973

Double mutants containing two Spo mutations concerned with different stages of sporulation were constructed. In these, the phenotype that is exhibited is that of the earlier sporulation block. The same procedure was applied to sporulation …

Evidence for induced synthesis of an active transport factor for mandelate in Pseudomonas putida Open

S. J. Higgins, J. Mandelstam · 1972

1. At low concentrations of mandelate there is a lag before induction of the mandelate regulon begins at a sub-maximum rate. Cells preinduced with a saturating concentration of inducer do not exhibit this lag when they are transferred to s…

Regulation of pathways degrading aromatic substrates in Pseudomonas putida. Enzymic response to binary mixtures of substrates Open

S. J. Higgins, J. Mandelstam · 1972

1. Induction constants (Kind) and repression constants (Krep), which are a measure of the affinity of the inducers or repressors for the induction systems, were measured for mandelate, benzoate and p-hydroxybenzoate in Pseudomonas putida. …

Sporulation in Bacillus subtilis. Effect of medium on the form of chromosome replication and on initiation to sporulation in Bacillus subtilis Open

J. Mandelstam, Janet M. Sterlini, D. Kay · 1971

Thymine-requiring mutants of Bacillus subtilis and mutants that are temperature-sensitive for initiation of chromosome replication have been used to study the relationship between sporulation and chromosome formation. The DNA synthesis tha…

Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells Open

A. R. Glenn, J. Mandelstam · 1971

1. The purification of the `vegetative' alkaline phosphatase of Bacillus subtilis 168 was simplified by ionic elution of the enzyme from intact cells. 2. The enzyme has a molecular weight of about 70000 and treatment of the enzyme with 10m…

Sporulation of Bacillus subtilis in Continuous Culture Open

Ian W. Dawes, J. Mandelstam · 1970

Sporulation of Bacillus subtilis 168 was studied in chemostat cultures. Sporulation occurred at high frequency under limitation of growth by glucose or the nitrogen source in minimal medium, whereas rates of sporulation were low for Mg 2+ …

Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogeneous mutants Open

W.M. Waites, D. Kay, Ian W. Dawes, David A. Wood, S. C. Warren , et al. · 1970

A comparison was made of morphological changes and successive, mainly biochemical, marker events for sporulation in 14 asporogenous mutants. The morphological and biochemical sequences are linked so that arrested development in one is acco…

Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance Open

Janet M. Sterlini, J. Mandelstam · 1969

1. Experiments to determine the point of commitment to sporulation were carried out by restoring nutrients at different times to suspensions of sporulating Bacillus subtilis. 2. No single point of commitment to the process as a whole was f…

Sporulation in Bacillus subtilis. The role of exoprotease Open

J. Mandelstam, W.M. Waites · 1968

1. Intracellular turnover of protein was measured in wild-type Bacillus subtilis, which produces exoprotease at stage I in the sporulation process. Protein is degraded at a rate of 8–10%/hr. 2. As a result of this turnover, the proteins of…

Induction and multi-sensitive end-product repression in two converging pathways degrading aromatic substances in Pseudomonas fluorescens Open

I.L. Stevenson, J. Mandelstam · 1965

1. Control of enzyme formation has been examined in the pathways degrading mandelate and p-hydroxymandelate in Pseudomonas fluorescens. 2. The first three enzymes form a group which is common to both pathways and which is co-ordinately ind…

INDUCTION AND MULTI-SENSITIVE END-PRODUCT REPRESSION IN THE ENZYMIC PATHWAY DEGRADING MANDELATE IN PSEUDOMONAS FLUORESCENS Open

J. Mandelstam, GA Jacoby · 1965

1. The first five enzymes involved in the degradation of mandelate in Pseudomonas fluorescens have been examined. 2. Induction is not significantly affected by glucose. 3. The first three enzymes form a group inducible by mandelate and rep…

SPECIFIC METABOLIC REPRESSION OF THREE INDUCED ENZYMES IN ESCHERICHIA COLI Open

Elizabeth McFall, J. Mandelstam · 1963

Research Article| November 01 1963 SPECIFIC METABOLIC REPRESSION OF THREE INDUCED ENZYMES IN ESCHERICHIA COLI E MCFALL; E MCFALL Search for other works by this author on: This Site PubMed Google Scholar J MANDELSTAM J MANDELSTAM Search for…

Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria Open

J. Mandelstam · 1962

Research Article| August 01 1962 Preparation and properties of the mucopeptides of cell walls of gram-negative bacteria J MANDELSTAM J MANDELSTAM Search for other works by this author on: This Site PubMed Google Scholar Author and article …

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