J. Monty Watkins
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View article: Outer Membrane Vesicles From <i>Bacteroides fragilis</i> Contain Coding and Non‐Coding Small RNA Species That Modulate Inflammatory Signalling in Intestinal Epithelial Cells
Outer Membrane Vesicles From <i>Bacteroides fragilis</i> Contain Coding and Non‐Coding Small RNA Species That Modulate Inflammatory Signalling in Intestinal Epithelial Cells Open
Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe‐derived RNAs carried by outer membrane vesicles (OMVs) t…
View article: A Live-Cell NanoBRET Assay to Monitor RNA–Protein Interactions and Their Inhibition by Small Molecules
A Live-Cell NanoBRET Assay to Monitor RNA–Protein Interactions and Their Inhibition by Small Molecules Open
View article: MERS-CoV antagonizes PKR activation by inhibiting its condensation at viral replication complexes
MERS-CoV antagonizes PKR activation by inhibiting its condensation at viral replication complexes Open
SUMMARY Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic virus that antagonizes innate immune responses, including the protein kinase R (PKR) pathway. Here, we examine the process of PKR activation in response…
View article: RNase L regulates the antiviral proteome by accelerating mRNA decay, inhibiting nuclear mRNA export, and repressing RNAPII-mediated transcription
RNase L regulates the antiviral proteome by accelerating mRNA decay, inhibiting nuclear mRNA export, and repressing RNAPII-mediated transcription Open
SUMMARY Ribonuclease L (RNase L) is an antiviral endoribonuclease that triggers widespread degradation of cellular mRNAs. Here, we show that the degradation of cellular mRNA by RNase L is a conserved response to flaviviruses, including Zik…
View article: Outer membrane vesicles from<i>Bacteroides fragilis</i>contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells
Outer membrane vesicles from<i>Bacteroides fragilis</i>contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells Open
Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe-derived RNAs carried by outer membrane vesicles (OMVs) t…
View article: Assessing the Suitability of Deubiquitylases As Substrates For Targeted Protein Degradation
Assessing the Suitability of Deubiquitylases As Substrates For Targeted Protein Degradation Open
Summary Deubiquitylases (DUBs) are a family of specialized proteases that hydrolyze the isopeptide bond between a lysine and the C-terminal carboxylate of Ubiquitin. DUBs are involved in a myriad of cellular processes and many are attracti…
View article: Twist-Induced Epithelial-to-Mesenchymal Transition Confers Specific Metabolic and Mitochondrial Alterations
Twist-Induced Epithelial-to-Mesenchymal Transition Confers Specific Metabolic and Mitochondrial Alterations Open
Cells undergo significant epigenetic and phenotypic change during the epithelial-to-mesenchymal transition (EMT), a process observed in development, wound healing, and cancer metastasis. EMT confers several advantageous characteristics, in…
View article: RNase L-induced bodies sequester subgenomic flavivirus RNAs to promote viral RNA decay
RNase L-induced bodies sequester subgenomic flavivirus RNAs to promote viral RNA decay Open
Subgenomic flavivirus RNAs (sfRNAs) are structured RNAs encoded by flaviviruses that promote viral infection by inhibiting cellular RNA decay machinery. Herein, we analyze sfRNA production and localization using single-molecule RNA fluores…
View article: A closer look at mammalian antiviral condensates
A closer look at mammalian antiviral condensates Open
Several biomolecular condensates assemble in mammalian cells in response to viral infection. The most studied of these are stress granules (SGs), which have been proposed to promote antiviral innate immune signaling pathways, including the…
View article: RNase L-induced bodies sequester subgenomic flavivirus RNAs and re-establish host RNA decay
RNase L-induced bodies sequester subgenomic flavivirus RNAs and re-establish host RNA decay Open
SUMMARY Subgenomic flavivirus RNAs (sfRNAs) are structured RNA elements encoded in the 3’-UTR of flaviviruses that promote viral infection by inhibiting cellular RNA decay machinery. Herein, we analyze the production of sfRNAs using single…
View article: Mechanisms and consequences of mRNA destabilization during viral infections
Mechanisms and consequences of mRNA destabilization during viral infections Open
View article: G3BP1-dependent condensation of translationally inactive viral RNAs antagonizes infection
G3BP1-dependent condensation of translationally inactive viral RNAs antagonizes infection Open
G3BP1 is an RNA binding protein that condenses untranslating messenger RNAs into stress granules (SGs). G3BP1 is inactivated by multiple viruses and is thought to antagonize viral replication by SG-enhanced antiviral signaling. Here, we sh…
View article: An Engineered Monovalent Anti–TNF-α Antibody with pH-Sensitive Binding Abrogates Immunogenicity in Mice following a Single Intravenous Dose
An Engineered Monovalent Anti–TNF-α Antibody with pH-Sensitive Binding Abrogates Immunogenicity in Mice following a Single Intravenous Dose Open
Therapeutic Abs directed toward TNF-α display significant immunogenicity in humans, frequently leading to lower serum concentrations of the Ab that are associated with lower treatment efficacy. The enhanced incidence of immunogenicity obse…