James R. DeVries
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View article: A general method for the induction and screening of antisera for cDNA-encoded polypeptides: antibodies specific for a coronavirus putative polymerase-encoding gene
A general method for the induction and screening of antisera for cDNA-encoded polypeptides: antibodies specific for a coronavirus putative polymerase-encoding gene Open
A prokaryotic vector, pGE374, containing the recA and lacZ genes, out-of-frame, was used for the expression of cDNA derived from the putative polymerase-encoding gene of the coronavirus mouse hepatitis virus strain A59 (MHV-A59). The pGE37…
View article: Genetic analysis of murine hepatitis virus strain JHM
Genetic analysis of murine hepatitis virus strain JHM Open
We performed a genetic analysis of 37 temperature-sensitive mutants of murine hepatitis virus strain JHM. Of our mutants, 32 did not induce murine hepatitis virus-specific RNA synthesis in infected cells at the restrictive temperature, 39 …
View article: Criteria for Affinity Chromatography of Steroid-binding Macromolecules
Criteria for Affinity Chromatography of Steroid-binding Macromolecules Open
When soluble fractions of rat kidney homogenates were exposed to affinity chromatography columns containing deoxycorticosterone covalently linked to an insoluble agarose matrix, aldosterone-binding activity was reduced by 92%. Similarly, w…
View article: <i>In Vitro</i> Formation of Assimilatory Reduced Nicotinamide Adenine Dinucleotide Phosphate: Nitrate Reductase from a <i>Neurospora</i> Mutant and a Component of Molybdenum-Enzymes
<i>In Vitro</i> Formation of Assimilatory Reduced Nicotinamide Adenine Dinucleotide Phosphate: Nitrate Reductase from a <i>Neurospora</i> Mutant and a Component of Molybdenum-Enzymes Open
An active Neurospora -like assimilatory NADPH-nitrate reductase (EC 1.6.6.2), which can be formed in vitro by incubation of extracts of nitrate-induced Neurospora crassa mutant nit-1 with extracts of ( a ) certain other nonallelic nitrate …