Edward J. A. Douglas
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View article: Polymyxin heteroresistance in <i>Klebsiella oxytoca</i>
Polymyxin heteroresistance in <i>Klebsiella oxytoca</i> Open
Antibiotic heteroresistance presents a growing public health concern since the phenotype is associated with treatment failure and is hard to detect using conventional diagnostic testing. In this study, we characterized polymyxin B heterore…
View article: Anti-staphylococcal fatty acids: mode of action, bacterial resistance and implications for therapeutic application
Anti-staphylococcal fatty acids: mode of action, bacterial resistance and implications for therapeutic application Open
Novel strategies to counter multidrug-resistant pathogens such as methicillin-resistant Staphylococcus aureus are urgently required. The antimicrobial properties of fatty acids (FAs) have long been recognized and offer significant promise …
View article: Polymyxin B lethality requires energy-dependent outer membrane disruption
Polymyxin B lethality requires energy-dependent outer membrane disruption Open
Polymyxin antibiotics target lipopolysaccharide (LPS) in both membranes of the bacterial cell envelope, leading to bacterial killing through a mechanism that remains poorly understood. Here, we demonstrate that metabolic activity is essent…
View article: Improved Antibacterial Activity of 1,3,4-Oxadiazole-Based Compounds That Restrict <i>Staphylococcus aureus</i> Growth Independent of LtaS Function
Improved Antibacterial Activity of 1,3,4-Oxadiazole-Based Compounds That Restrict <i>Staphylococcus aureus</i> Growth Independent of LtaS Function Open
The lipoteichoic acid (LTA) biosynthesis pathway has emerged as a promising antimicrobial therapeutic target. Previous studies identified the 1,3,4 oxadiazole compound 1771 as an LTA inhibitor with activity against Gram-positive pathogens.…
View article: Staph wars: the antibiotic pipeline strikes back
Staph wars: the antibiotic pipeline strikes back Open
Antibiotic chemotherapy is widely regarded as one of the most significant medical advancements in history. However, the continued misuse of antibiotics has contributed to the rapid rise of antimicrobial resistance (AMR) globally. Staphyloc…
View article: Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functional genomics approach to identify a …
View article: Author Response: Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Author Response: Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
Full text Figures and data Side by side Abstract eLife assessment Introduction Results Discussion Materials and methods Data availability References Peer review Author response Article and author information Metrics Abstract The bloodstrea…
View article: Author Response: Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Author Response: Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functional genomics approach to identify a …
View article: Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
Introductory Paragraph / Abstract The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functiona…
View article: Novel antimicrobial strategies to treat multi‐drug resistant <i>Staphylococcus aureus</i> infections
Novel antimicrobial strategies to treat multi‐drug resistant <i>Staphylococcus aureus</i> infections Open
Antimicrobial resistance is a major obstacle for the treatment of infectious diseases and currently represents one of the most significant threats to global health. Staphylococcus aureus remains a formidable human pathogen with high mortal…
View article: Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functional genomics approach to identify a …
View article: Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia
Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia Open
Introductory Paragraph / Abstract The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functiona…
View article: Extensive re-modelling of the cell wall during the development of <i>Staphylococcus aureus</i> bacteraemia
Extensive re-modelling of the cell wall during the development of <i>Staphylococcus aureus</i> bacteraemia Open
Introductory Paragraph / Abstract The bloodstream represents a hostile environment that bacteria must overcome to cause bacteraemia. To understand how the major human pathogen Staphylococcus aureus manages this we have utilised a functiona…
View article: LoCKAmp: lab-on-PCB technology for <3 minute virus genetic detection
LoCKAmp: lab-on-PCB technology for <3 minute virus genetic detection Open
LoCKAmp: integrated LAMP genetic amplification device demonstrating reliable detection and ultrafast sample-to-answer time.
View article: Antibacterial activity of novel linear polyamines against Staphylococcus aureus
Antibacterial activity of novel linear polyamines against Staphylococcus aureus Open
New therapeutic options are urgently required for the treatment of Staphylococcus aureus infections. Accordingly, we sought to exploit the vulnerability of S. aureus to naturally occurring polyamines. We have developed and tested the anti-…
View article: Wall Teichoic Acids Facilitate the Release of Toxins from the Surface of Staphylococcus aureus
Wall Teichoic Acids Facilitate the Release of Toxins from the Surface of Staphylococcus aureus Open
The production and release of cytolytic toxins is a critical aspect for the pathogenicity of many bacterial pathogens. In this study, we demonstrate a role for wall teichoic acids, molecules that are anchored to the peptidoglycan of the ba…
View article: Wall teichoic acids facilitate the release of toxins from the surface of <i>Staphylococcus aureus</i>
Wall teichoic acids facilitate the release of toxins from the surface of <i>Staphylococcus aureus</i> Open
A major feature of the pathogenicity of Staphylococcus aureus is its ability to secrete cytolytic toxins. This process involves the translocation of the toxins from the cytoplasm, through the bacterial membrane and the cell wall to the ext…
View article: The MpsB protein contributes to both the toxicity and immune evasion capacity of Staphylococcus aureus
The MpsB protein contributes to both the toxicity and immune evasion capacity of Staphylococcus aureus Open
Understanding the role specific bacterial factors play in the development of severe disease in humans is critical if new approaches to tackle such infections are to be developed. In this study we focus on genes we have found to be associat…
View article: Self-sensitisation of <i>Staphylococcus aureus</i> to the antimicrobial factors found in human blood
Self-sensitisation of <i>Staphylococcus aureus</i> to the antimicrobial factors found in human blood Open
Introductory Paragraph For opportunistic pathogens, the switch from a commensal to an invasive lifestyle is often considered an accidental event. But with plentiful opportunity, what leads one accidental event to result in an invasive infe…
View article: The MpsB protein contributes to both the toxicity and immune evasion capacity of <i>Staphylococcus aureus</i>
The MpsB protein contributes to both the toxicity and immune evasion capacity of <i>Staphylococcus aureus</i> Open
Understanding the role specific bacterial factors play in the development of severe disease in humans is critical if new approaches to tackle such infections are to be developed. In this study we focus on genes we have found to be associat…
View article: Resistance to Ceftazidime/Avibactam plus Meropenem/Vaborbactam When Both Are Used Together Is Achieved in Four Steps in Metallo-β-Lactamase-Negative Klebsiella pneumoniae
Resistance to Ceftazidime/Avibactam plus Meropenem/Vaborbactam When Both Are Used Together Is Achieved in Four Steps in Metallo-β-Lactamase-Negative Klebsiella pneumoniae Open
Serine β-lactamases are dominant causes of β-lactam resistance in Klebsiella pneumoniae isolates. Recently, this has driven clinical deployment of the β-lactam–β-lactamase inhibitor pairs ceftazidime/avibactam and meropenem/vaborbactam. We…
View article: Mutation of <i>kvrA</i> Causes OmpK35 and OmpK36 Porin Downregulation and Reduced Meropenem-Vaborbactam Susceptibility in KPC-Producing Klebsiella pneumoniae
Mutation of <i>kvrA</i> Causes OmpK35 and OmpK36 Porin Downregulation and Reduced Meropenem-Vaborbactam Susceptibility in KPC-Producing Klebsiella pneumoniae Open
Meropenem-vaborbactam resistance in Klebsiella pneumoniae isolates is associated with loss-of-function mutations in the OmpK35 and OmpK36 porins. We identify two previously unknown loss-of-function mutations that confer cefuroxime resistan…
View article: Resistance to Ceftazidime/Avibactam Plus Meropenem/Vaborbactam When Both are Used Together Achieved in Four Steps From Metallo-β-Lactamase Negative<i>Klebsiella pneumoniae</i>
Resistance to Ceftazidime/Avibactam Plus Meropenem/Vaborbactam When Both are Used Together Achieved in Four Steps From Metallo-β-Lactamase Negative<i>Klebsiella pneumoniae</i> Open
Serine cephalosporinases and carbapenemases are dominant causes of critically important β-lactam resistance in Klebsiella pneumoniae . This has led to the recent clinical deployment of new serine β-lactamase inhibitors used in combination …
View article: Mutation of <i>kvrA</i> causes OmpK35/36 porin downregulation and reduced meropenem/vaborbactam susceptibility in KPC-producing <i>Klebsiella pneumoniae</i>
Mutation of <i>kvrA</i> causes OmpK35/36 porin downregulation and reduced meropenem/vaborbactam susceptibility in KPC-producing <i>Klebsiella pneumoniae</i> Open
Meropenem/vaborbactam resistance in Klebsiella pneumoniae is associated with loss of function mutations in the OmpK36 porin. Here we identify two previously unknown loss of function mutations that confer cefuroxime resistance in K. pneumon…