Jack E. Bramham
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View article: Assessing Photostability of mAb Formulations In Situ Using Light-Coupled NMR Spectroscopy
Assessing Photostability of mAb Formulations In Situ Using Light-Coupled NMR Spectroscopy Open
Biopharmaceuticals, such as monoclonal antibodies (mAbs), need to maintain their chemical and physical stability in formulations throughout their lifecycle. It is known that exposure of mAbs to light, particularly UV, triggers chemical and…
View article: Photochemistry of Ru(II) Triazole Complexes with 6-Membered Chelate Ligands: Detection and Reactivity of Ligand-Loss Intermediates
Photochemistry of Ru(II) Triazole Complexes with 6-Membered Chelate Ligands: Detection and Reactivity of Ligand-Loss Intermediates Open
Photochemical ligand release from metal complexes may be exploited in the development of novel photoactivated chemotherapy agents for the treatment of cancer and other diseases. Highly intriguing photochemical behavior is reported for two …
View article: Simple and effective <i>in situ</i> sample illumination for electron paramagnetic resonance
Simple and effective <i>in situ</i> sample illumination for electron paramagnetic resonance Open
A novel sample illumination approach for electron paramagnetic resonance, using the walls of the EPR tube as a light guide.
View article: Lighting up spin systems: enhancing characteristic <sup>1</sup>H signal patterns of fluorinated molecules
Lighting up spin systems: enhancing characteristic <sup>1</sup>H signal patterns of fluorinated molecules Open
Combining sample illumination and fluorine-edited selective TOCSY acquisition allows significant enhancements of characteristic 1 H signal fingerprints of fluorinated molecules.
View article: Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy
Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Open
Liquid-liquid phase separation (LLPS) of protein solutions is increasingly recognised as an important phenomenon in cell biology and biotechnology. However, opalescence and concentration fluctuations render LLPS difficult to study, particu…
View article: Controlled release and characterisation of photocaged molecules using <i>in situ</i> LED illumination in solution NMR spectroscopy
Controlled release and characterisation of photocaged molecules using <i>in situ</i> LED illumination in solution NMR spectroscopy Open
We demonstrate that photo-uncaging reactions triggered by LED illumination can be conveniently monitored in situ by solution NMR, offering new ways to characterise and optimise photocages.
View article: Universal approach to in situ light-coupled NMR spectroscopy without fibre optics
Universal approach to in situ light-coupled NMR spectroscopy without fibre optics Open
In situ illumination of liquid-state nuclear magnetic resonance (NMR) samples makes it possible for a wide range of light-dependent chemical and biological phenomena to be studied by the powerful analytical technique. However, the position…
View article: Stability of a high-concentration monoclonal antibody solution produced by liquid–liquid phase separation
Stability of a high-concentration monoclonal antibody solution produced by liquid–liquid phase separation Open
Subcutaneous injection of a low volume (100 mg/mL) formulation is an attractive administration strategy for monoclonal antibodies (mAbs) and other biopharmaceutical proteins. Using concentrated solutions may also be beneficial at various s…
View article: Comprehensive Assessment of Protein and Excipient Stability in Biopharmaceutical Formulations Using <sup>1</sup>H NMR Spectroscopy
Comprehensive Assessment of Protein and Excipient Stability in Biopharmaceutical Formulations Using <sup>1</sup>H NMR Spectroscopy Open
Biopharmaceutical proteins are important drug therapies in the treatment of a range of diseases. Proteins, such as antibodies (Abs) and peptides, are prone to chemical and physical degradation, particularly at the high concentrations curre…
View article: <sup>19</sup>F Dark-State Exchange Saturation Transfer NMR Reveals Reversible Formation of Protein-Specific Large Clusters in High-Concentration Protein Mixtures
<sup>19</sup>F Dark-State Exchange Saturation Transfer NMR Reveals Reversible Formation of Protein-Specific Large Clusters in High-Concentration Protein Mixtures Open
Proteins frequently exist as high-concentration mixtures, both in biological environments and increasingly in biopharmaceutical co-formulations. Such crowded conditions promote protein-protein interactions, potentially leading to formation…
View article: Investigating Liquid–Liquid Phase Separation of a Monoclonal Antibody Using Solution-State NMR Spectroscopy: Effect of Arg·Glu and Arg·HCl
Investigating Liquid–Liquid Phase Separation of a Monoclonal Antibody Using Solution-State NMR Spectroscopy: Effect of Arg·Glu and Arg·HCl Open
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense (protein-rich) and diluted (protein-lean) phases and should be avoided in the final drug product. Understanding the …