Jack R. Harkema
YOU?
Author Swipe
View article: Mechanisms of sex differences in acute and long COVID sequelae in mice
Mechanisms of sex differences in acute and long COVID sequelae in mice Open
While males are more likely to suffer severe outcomes during acute COVID-19, a greater proportion of females develop post-acute sequalae of COVID-19 (PASC) despite similar rates of infection. To identify mechanisms of PASC, mice were infec…
View article: Preferential apical infection and spread of human metapneumovirus highlights the importance of inhaled delivery of neutralizing monoclonal antibody to treat established infections
Preferential apical infection and spread of human metapneumovirus highlights the importance of inhaled delivery of neutralizing monoclonal antibody to treat established infections Open
Human metapneumovirus (hMPV) is a frequent cause of acute respiratory infections in infants, the elderly, and the immunocompromised. No vaccines or treatments are currently available beyond supportive care. Building on our previous work wi…
View article: Evaluating the role of alveolar macrophages in tolerance to ozone
Evaluating the role of alveolar macrophages in tolerance to ozone Open
Acute exposure to ozone (O3) causes pulmonary inflammation and injury in humans and animal models. In rodents, acute O3-induced inflammation and injury can be mitigated by pre-exposure to relatively low concentrations of O3, a phenomenon r…
View article: Shift in the urinary metabolome associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin activation of the hepatic aryl hydrocarbon receptor
Shift in the urinary metabolome associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin activation of the hepatic aryl hydrocarbon receptor Open
View article: Mouse Adapted Omicron BA.5 Induces A Fibrotic Lung Disease Phenotype in BALB/c Mice.
Mouse Adapted Omicron BA.5 Induces A Fibrotic Lung Disease Phenotype in BALB/c Mice. Open
Following SARS-CoV-2 Omicron BA.1, subsequent Omicron sub-lineages have continued to emerge, challenging the development of intervention and prevention strategies, including monoclonal antibodies and vaccines. To better understand the path…
View article: Vertical Transmission of Bovine H5N1 Influenza Virus during Pregnancy and Lactation in Mice
Vertical Transmission of Bovine H5N1 Influenza Virus during Pregnancy and Lactation in Mice Open
Summary Panzootic spillover of H5N1 virus clade 2.3.4.4b has resulted in expanded host range among placental mammals, with lactation transmission via milk documented. Whether infection during pregnancy leads to in utero or lactational vert…
View article: Omega-3 Fatty Acid Synergy with Glucocorticoid in Lupus Macrophages: Targeting Pathogenic Pathways to Reduce Steroid Dependence
Omega-3 Fatty Acid Synergy with Glucocorticoid in Lupus Macrophages: Targeting Pathogenic Pathways to Reduce Steroid Dependence Open
Introduction Systemic lupus erythematosus (SLE) is a complex autoimmune disorder characterized by aberrant inflammation, type I IFN-stimulated gene (ISG) expression, and autoantibody production. Glucocorticoids (GCs) like dexamethasone (DE…
View article: Designed miniproteins potently inhibit and protect against MERS-CoV
Designed miniproteins potently inhibit and protect against MERS-CoV Open
View article: A new mouse model for ozone health effects research
A new mouse model for ozone health effects research Open
The CC002/Unc strain provides an improved model to study the effects of acute and repeated O3 exposure due to its enhanced sensitivity vs. C57BL/6J and more human-like thermoregulatory response. Further genetic analysis to pinpo…
View article: Designed miniproteins potently inhibit and protect against MERS-CoV
Designed miniproteins potently inhibit and protect against MERS-CoV Open
View article: Correction: Central IKKβ Inhibition prevents air pollution mediated peripheral inflammation and exaggeration of type II diabetes
Correction: Central IKKβ Inhibition prevents air pollution mediated peripheral inflammation and exaggeration of type II diabetes Open
View article: 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) elicited dose-dependent shifts in the murine urinary metabolome associated with hepatic AHR-mediated differential gene expression
2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin (TCDD) elicited dose-dependent shifts in the murine urinary metabolome associated with hepatic AHR-mediated differential gene expression Open
Epidemiological evidence suggests an association between dioxin and dioxin-like compound (DLC) exposure and human liver disease. The prototypical DLC, 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD), has been shown to induce the progression o…
View article: Disruption of canonical AHR-mediated induction of hepatocyte PKM2 expression compromises antioxidant defenses and increases TCDD-induced hepatotoxicity
Disruption of canonical AHR-mediated induction of hepatocyte PKM2 expression compromises antioxidant defenses and increases TCDD-induced hepatotoxicity Open
Metabolic reprogramming by the pyruvate kinase M2 isoform is associated with cell proliferation and reactive oxygen species (ROS) defenses. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an environmental contaminant that induces ROS and hepat…
View article: Impact of soluble epoxide hydrolase inhibition on silica-induced pulmonary fibrosis, ectopic lymphoid neogenesis, and autoantibody production in lupus-prone mice
Impact of soluble epoxide hydrolase inhibition on silica-induced pulmonary fibrosis, ectopic lymphoid neogenesis, and autoantibody production in lupus-prone mice Open
cSiO2 evoked robust pulmonary inflammation and fibrosis in NZBWF1 mice that was evident at 7 days PI and progressed to ELS development and autoimmunity by 28 days PI. sEH inhibition by TPPU modestly suppressed cSiO2-i…
View article: Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice
Subchronic intranasal lipopolysaccharide exposure induces pulmonary autoimmunity and glomerulonephritis in NZBWF1 mice Open
Lupus, a systemic autoimmune disease shaped by gene-environment interplay, often progresses to endstage renal failure. While subchronic systemic exposure to bacterial lipopolysaccharide (LPS) triggers autoimmunity and glomerulonephritis in…
View article: Alveolar macrophages play a key role in tolerance to ozone
Alveolar macrophages play a key role in tolerance to ozone Open
Acute exposure to ozone (O 3 ) causes pulmonary inflammation and injury in humans and animal models. In rodents, acute O 3 -induced inflammation and injury can be mitigated by pre-exposure to relatively low concentration O 3 , a phenomenon…
View article: Crystalline silica-induced pulmonary inflammation and autoimmunity in mature adult NZBW/f1 mice: age-related sensitivity and impact of omega-3 fatty acid intervention
Crystalline silica-induced pulmonary inflammation and autoimmunity in mature adult NZBW/f1 mice: age-related sensitivity and impact of omega-3 fatty acid intervention Open
These results highlight life-stage significance in this preclinical lupus model and underscore omega-3 fatty acids' therapeutic potential against toxicant-triggered autoimmune responses.
View article: Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice
Dietary docosahexaenoic acid supplementation inhibits acute pulmonary transcriptional and autoantibody responses to a single crystalline silica exposure in lupus-prone mice Open
Introduction Workplace exposure to respirable crystalline silica (cSiO 2 ) has been epidemiologically linked to lupus. Consistent with this, repeated subchronic intranasal cSiO 2 instillation in lupus-prone NZBWF1 mice induces inflammation…
View article: Divergent pathogenetic outcomes in BALB/c mice following Omicron subvariant infection
Divergent pathogenetic outcomes in BALB/c mice following Omicron subvariant infection Open
Following the emergence of B.1.1.529 Omicron, the SARS-CoV-2 virus evolved into a significant number of sublineage variants that possessed numerous mutations throughout the genome, but particularly within the spike glycoprotein (S) gene. F…
View article: Characterizing the impact of simvastatin co-treatment of cell specific TCDD-induced gene expression and systemic toxicity
Characterizing the impact of simvastatin co-treatment of cell specific TCDD-induced gene expression and systemic toxicity Open
2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) is associated with metabolic syndrome (MetS) in humans and elicits pathologies in rodents that resemble non-alcoholic fatty liver disease (NAFLD) in humans through activation of the aryl hydroca…
View article: Lipidome modulation by dietary omega-3 polyunsaturated fatty acid supplementation or selective soluble epoxide hydrolase inhibition suppresses rough LPS-accelerated glomerulonephritis in lupus-prone mice
Lipidome modulation by dietary omega-3 polyunsaturated fatty acid supplementation or selective soluble epoxide hydrolase inhibition suppresses rough LPS-accelerated glomerulonephritis in lupus-prone mice Open
Introduction Lipopolysaccharide (LPS)-accelerated autoimmune glomerulonephritis (GN) in NZBWF1 mice is a preclinical model potentially applicable for investigating lipidome-modulating interventions against lupus. LPS can be expressed as on…
View article: Prevention of bleomycin‐induced lung fibrosis via inhibition of the MRTF/SRF transcription pathway
Prevention of bleomycin‐induced lung fibrosis via inhibition of the MRTF/SRF transcription pathway Open
Bleomycin‐induced lung fibrosis is a debilitating disease, linked to high morbidity and mortality in chemotherapy patients. The MRTF/SRF transcription pathway has been proposed as a potential therapeutic target, as it is critical for myofi…
View article: Single cell analysis of docosahexaenoic acid suppression of sequential LPS-induced proinflammatory and interferon-regulated gene expression in the macrophage
Single cell analysis of docosahexaenoic acid suppression of sequential LPS-induced proinflammatory and interferon-regulated gene expression in the macrophage Open
Preclinical and clinical studies suggest that consumption of long chain omega-3 polyunsaturated fatty acids (PUFAs) reduces severity of chronic inflammatory and autoimmune diseases. While these ameliorative effects are conventionally assoc…
View article: Aerosol physicochemical determinants of carbon black and ozone inhalation co-exposure induced pulmonary toxicity
Aerosol physicochemical determinants of carbon black and ozone inhalation co-exposure induced pulmonary toxicity Open
Air pollution accounts for more than 7 million premature deaths worldwide. Using ultrafine carbon black (CB) and ozone (O3) as a model for an environmental co-exposure scenario, the dose response relationships in acute pulmonary injury and…
View article: Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus
Comparative effects of human-equivalent low, moderate, and high dose oral prednisone intake on autoimmunity and glucocorticoid-related toxicity in a murine model of environmental-triggered lupus Open
Autoimmune diseases can be triggered by environmental toxicants such as crystalline silica dust (cSiO 2 ). Here, we characterized the dose-dependent immunomodulation and toxicity of the glucocorticoid (GC) prednisone in a preclinical model…
View article: Science-based Policy Recommendations for Fine Particulate Matter in the United States
Science-based Policy Recommendations for Fine Particulate Matter in the United States Open
International audience
View article: A Locus on Chromosome 15 Contributes to Acute Ozone-induced Lung Injury in Collaborative Cross Mice
A Locus on Chromosome 15 Contributes to Acute Ozone-induced Lung Injury in Collaborative Cross Mice Open
Ozone (O3)-induced respiratory toxicity varies considerably within the human population and across inbred mouse strains, indicative of gene-environment interactions (GxE). Though previous studies have identified several quantita…
View article: Inflammation as a Key Outcome Pathway in Particle Induced Effects in the Lung
Inflammation as a Key Outcome Pathway in Particle Induced Effects in the Lung Open
Inflammation is considered a key event in the pathology of many chronic diseases, including pulmonary and systemic particle induced effects. In addition, inflammation is now considered as the key response in standard setting for poorly-sol…
View article: In Vitro and In Vivo Analysis of Extracellular Vesicle‐Mediated Metastasis Using a Bright, Red‐Shifted Bioluminescent Reporter Protein (Advanced Genetics 1/03)
In Vitro and In Vivo Analysis of Extracellular Vesicle‐Mediated Metastasis Using a Bright, Red‐Shifted Bioluminescent Reporter Protein (Advanced Genetics 1/03) Open
Extracellular Vesicle Imaging Cancer cell‐derived extracellular vesicles (EVs) promote tumor growth and spread. Studying the distribution of EVs in the body is key to understanding cancer progression and developing therapies. In article nu…
View article: Silica Induction of Diverse Inflammatory Proteome in Lungs of Lupus-Prone Mice Quelled by Dietary Docosahexaenoic Acid Supplementation
Silica Induction of Diverse Inflammatory Proteome in Lungs of Lupus-Prone Mice Quelled by Dietary Docosahexaenoic Acid Supplementation Open
Repeated short-term intranasal instillation of lupus-prone mice with crystalline silica (cSiO 2 ) induces inflammatory gene expression and ectopic lymphoid neogenesis in the lung, leading to early onset of systemic autoimmunity and rapid p…