Explanipedia

Abbreviations Open

J. Throck Watson, O. David Sparkman · 2007

Index Open

J. Throck Watson, O. David Sparkman · 2007

Characterization of peptide folding nuclei by hydrogen/deuterium exchange‐mass spectrometry Open

Xue Li, Robin J. Hood, William J. Wedemeyer, J. Throck Watson · 2005

Covalently linked pairs of well‐chosen peptides can be good model systems for protein folding studies because they can adopt stable secondary, side‐chain, and tertiary structure under certain conditions. We demonstrate a method for charact…

Hsp90/p50cdc37 Is Required for Mixed-lineage Kinase (MLK) 3 Signaling Open

Hua Zhang, Wei Wu, Yan Du, Sarah J. Santos, Susan E. Conrad , et al. · 2004

Mixed-lineage kinase 3 (MLK3) is a mitogen-activated protein kinase (MAPK) kinase kinase that activates MAPK pathways, including the c-Jun NH(2)-terminal kinase (JNK) and p38 pathways. MLK3 and its family members have been implicated in JN…

Recognition of cysteine‐containing peptides through prompt fragmentation of the 4‐dimethylaminophenylazophenyl‐4′‐maleimide derivative during analysis by MALDI‐MS Open

Chad R. Borges, J. Throck Watson · 2003

Under specified UV‐MALDI conditions, the 4‐dimethylaminophenylazophenyl‐4'‐maleimide (DABMI) derivative of cysteine‐containing peptides undergoes prompt fragmentation to produce a characteristic mass spectral pattern. As reported previousl…

Dopamine biosynthesis is regulated byS-glutathionylation. Potential mechanism of tyrosine hydroxylase inhibition under oxidative stress. Open

Chad R. Borges, Timothy J. Geddes, J. Throck Watson, Donald M. Kuhn · 2003

Page 48299, Fig. 6: The mass label for the largest peak on row C (upper row) was cut off. It should be labeled at 2464.23. Page 48300, Fig. 7: The lines of the spectra are not visible. Both figures are printed correctly below.

Dopamine Biosynthesis Is Regulated byS-Glutathionylation Open

Chad R. Borges, Timothy J. Geddes, J. Throck Watson, Donald M. Kuhn · 2002

Tyrosine hydroxylase (TH), the initial and rate-limiting enzyme in the biosynthesis of the neurotransmitter dopamine, is inhibited by the sulfhydryl oxidant diamide in a concentration-dependent manner. The inhibitory effect of diamide on T…

Peroxynitrite-induced Nitration of Tyrosine Hydroxylase Open

Donald M. Kuhn, Mahdieh Sadidi, Xiuli Liu, Christian W. Kreipke, Timothy J. Geddes , et al. · 2002

Tyrosine hydroxylase (TH), the initial and rate-limiting enzyme in the biosynthesis of the neurotransmitter dopamine, is inactivated by peroxynitrite. The sites of peroxynitrite-induced tyrosine nitration in TH have been identified by matr…

Vision 2020: 2000 Separations Roadmap Open

Stephen L. Adler, Earl Beaver, Paul Bryan, S.M. Robinson, J. Throck Watson · 2000

This report documents the results of four workshops on the technology barriers, research needs, and priorities of the chemical, agricultural, petroleum, and pharmaceutical industries as they relate to separation technologies utilizing adso…

Probing the Folding Pathways of Long R3Insulin-like Growth Factor-I (LR3IGF-I) and IGF-I via Capture and Identification of Disulfide Intermediates by Cyanylation Methodology and Mass Spectrometry Open

Ying Yang, Jiang Wu, J. Throck Watson · 1999

This report describes an integrated investigation of the refolding and reductive unfolding of insulin-like growth factor (IGF-I) and its variant, long R(3) IGF-I (LR(3)IGF-I), which has a Glu(3) to Arg(3) substitution and a hydrophobic 13-…

Trapping of intermediates during the refolding of recombinant human epidermal growth factor (hEGF) by cyanylation, and subsequent structural elucidation by mass spectrometry Open

Jiang Wu, YingYang, J. Throck Watson · 1998

Human epidermal growth factor (hEGF) contains 53 amino acids and three disulfide bonds. The unfolded, reduced hEGF is allowed to refold under mildly alkaline conditions. The folding is quenched at different time points by adjusting the pH …

A novel methodology for assignment of disulfide bond pairings in proteins Open

Jiang Wu, J. Throck Watson · 1997

A novel methodology is described for the assignment of disulfide bonds in proteins of known sequence. The denatured protein is subjected to limited reduction by tris(2‐carboxyethyl)phosphine (TCEP) in pH 3.0 citrate buffer to produce a mix…

Fatty Acid Substrate Specificities of Human Prostaglandin-endoperoxide H Synthase-1 and −2 Open

Odette Laneuville, Debra K. Breuer, Naxing Xu, Zhiheng Huang, Douglas A. Gage , et al. · 1995

Human prostaglandin-endoperoxide H synthase-1 and -2 (hPGHS-1 and hPGHS-2) were expressed by transient transfection of COS-1 cells. Microsomes prepared from the transfected cells were used to measure the rates of oxygenation of several 18-…

Highly resolving two-dimensional gels for protein sequencing. Open

Samir Hanash, John R. Strahler, James V. Neel, Nabil Hailat, R Melhem , et al. · 1991

Two-dimensional (2D) PAGE, using carrier ampholytes for the first-dimension separation, has provided a tool for the simultaneous analysis of cellular proteins. To extend the utility of 2D PAGE to the preparative level, we have investigated…

Erratum to: A study of the gas-phase reaction between protonated acetaldehyde and reaction between protonated acetaldehyde and methanol Open

Gregory G. Dolnikowski, Timothy G. Heath, J. Throck Watson, James H. Scrivens, Christian Rolando · 1991

riate that W. E

Subject Index Open

Clarence H. Suelter, J. Throck Watson · 1990

residue I I USSCS, table of, 184 struchucs of, 147 Ammoni.. as CI reagent gas.74.75

Cumulative Subject Index Open

Clarence H. Suelter, J. Throck Watson · 1990

Urinary prostaglandins. Identification and origin. Open

Jürgen C. Frölich, Tracey Wilson, Brian J. Sweetman, M D Smigel, Alan S. Nies , et al. · 1975

Human urine was analyzed by mass spectrometry for the presence of prostaglandins. Prostaglandin E2 and F2alpha were detected in urine from females by selected ion monitoring of the prostaglandin E2-methylester-methoxime bis-acetate and the…

Effects of a False Neurotransmitter, p-Hydroxynorephedrine, on the Function of Adrenergic Neurons in Hypertensive Patients Open

Robert E. Rangno, John S. Kaufmann, John H. Cavanaugh, Donald P. Island, J. Throck Watson , et al. · 1973

Previous studies have shown that amphetamine and p-hydroxyamphetamine impair adrenergic transmission, and it has been suggested that this effect is mediated by an active metabolite, p-hydroxynorephedrine (PHN). Studies in experimental anim…

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