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View article: Beyond the Host: Evaluating Methods for Environmental Biosurveillance Ofviruses Using Avian Influenza Virus as a Case Study
Beyond the Host: Evaluating Methods for Environmental Biosurveillance Ofviruses Using Avian Influenza Virus as a Case Study Open
Environmental DNA and RNA-based virus biosurveillance holds promise as an early warningsystem for detecting pathogen incursions and tracking their evolution across fields. Unliketraditional pathogen monitoring, which typically relies on di…
View article: <scp>CRISPR</scp>‐Based Environmental Biosurveillance Assisted via Artificial Intelligence Design of Guide‐<scp>RNAs</scp>
<span>CRISPR</span>‐Based Environmental Biosurveillance Assisted via Artificial Intelligence Design of Guide‐<span>RNAs</span> Open
Environmental biosecurity challenges are intensifying as climate change and human activities accelerate the spread of invasive species, disrupting ecosystem composition, function, and essential services. Environmental DNA (eDNA) has transf…
View article: Whole Transcriptome RNA-Seq Reveals Drivers of Pathological Dysfunction in a Transgenic Model of Alzheimer’s Disease
Whole Transcriptome RNA-Seq Reveals Drivers of Pathological Dysfunction in a Transgenic Model of Alzheimer’s Disease Open
Alzheimer’s disease (AD) affects more than 55 million people worldwide, yet current theories cannot fully explain its aetiology. Accordingly, gene expression profiling has been used to provide a holistic view of the biology underpinning AD…
View article: A cry for kelp: Evidence for polyphenolic inhibition of Oxford Nanopore sequencing of brown algae
A cry for kelp: Evidence for polyphenolic inhibition of Oxford Nanopore sequencing of brown algae Open
Genomic resources have yielded unprecedented insights into ecological and evolutionary processes, not to mention their importance in economic and conservation management of specific organisms. However, the field of macroalgal genomics is h…
View article: Unlocking Antarctic molecular time-capsules -- recovering historical environmental DNA from museum-preserved sponges
Unlocking Antarctic molecular time-capsules -- recovering historical environmental DNA from museum-preserved sponges Open
Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high tempor…
View article: Unlocking Antarctic molecular time‐capsules – Recovering historical environmental <scp>DNA</scp> from museum‐preserved sponges
Unlocking Antarctic molecular time‐capsules – Recovering historical environmental <span>DNA</span> from museum‐preserved sponges Open
Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high tempor…
View article: Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems
Streamlining large-scale oceanic biomonitoring using passive eDNA samplers integrated into vessel's continuous pump underway seawater systems Open
Passive samplers are enabling the scaling of environmental DNA (eDNA) biomonitoring in our oceans, by circumventing the time-consuming process of water filtration. Designing a novel passive sampler that does not require extensive sample ha…
View article: A Cry For Kelp: Evidence for Polyphenolic Inhibition of Oxford Nanopore Sequencing of Brown Algae
A Cry For Kelp: Evidence for Polyphenolic Inhibition of Oxford Nanopore Sequencing of Brown Algae Open
Genomic resources for macroalgae are increasingly important for conservation and commercial management, however the generation of such resources continues to be hampered by difficulties in the isolation of suitable DNA. Even when DNA has b…
View article: Unlocking Antarctic molecular time-capsules – recovering historical environmental DNA from museum-preserved sponges
Unlocking Antarctic molecular time-capsules – recovering historical environmental DNA from museum-preserved sponges Open
Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high tempor…
View article: Whole transcriptome RNA-Seq reveals drivers of pathological dysfunction in a transgenic model of Alzheimer’s disease
Whole transcriptome RNA-Seq reveals drivers of pathological dysfunction in a transgenic model of Alzheimer’s disease Open
Alzheimer’s disease (AD) affects more than 55 million people worldwide, yet current theories cannot fully explain its aetiology. Accordingly, gene expression profiling has been used to provide a holistic view of the biology underpinning AD…
View article: Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge <scp>eDNA</scp> surveys
Unveiling the hidden diversity of marine eukaryotes in the Ross Sea: A comparative analysis of seawater and sponge <span>eDNA</span> surveys Open
The Ross Sea, Antarctica, while largely pristine, is experiencing increased anthropogenic pressures, necessitating enhanced biomonitoring efforts for conservation purposes. Environmental DNA (eDNA) extracted from marine sponges provides a …
View article: Characterizing Antarctic fish assemblages using eDNA obtained from marine sponge bycatch specimens
Characterizing Antarctic fish assemblages using eDNA obtained from marine sponge bycatch specimens Open
International conservation goals have been set to mitigate Southern Ocean ecosystem deterioration, with multiple monitoring programs evaluating progress towards those goals. The scale of continuous monitoring through visual observations, h…
View article: Moving environmental <scp>DNA</scp> (eDNA) technologies from benchtop to the field using passive sampling and <scp>PDQeX</scp> extraction
Moving environmental <span>DNA</span> (eDNA) technologies from benchtop to the field using passive sampling and <span>PDQeX</span> extraction Open
Environmental DNA (eDNA) metabarcoding has shown great promise as an effective, non‐invasive monitoring method for marine biomes. However, long filtration times and the need for state‐of‐the‐art laboratories are restricting sample replicat…
View article: Characterization of the First SARS-CoV-2 Isolates from Aotearoa New Zealand as Part of a Rapid Response to the COVID-19 Pandemic
Characterization of the First SARS-CoV-2 Isolates from Aotearoa New Zealand as Part of a Rapid Response to the COVID-19 Pandemic Open
SARS-CoV-2, the virus responsible for the COVID-19 pandemic, has wreaked havoc across the globe for the last two years. More than 300 million cases and over 5 million deaths later, we continue battling the first real pandemic of the 21st c…
View article: Uncoupling Molecular Testing for SARS-CoV-2 From International Supply Chains
Uncoupling Molecular Testing for SARS-CoV-2 From International Supply Chains Open
The rapid global rise of COVID-19 from late 2019 caught major manufacturers of RT-qPCR reagents by surprise and threw into sharp focus the heavy reliance of molecular diagnostic providers on a handful of reagent suppliers. In addition, loc…
View article: Rapid Response to SARS-CoV-2 in Aotearoa New Zealand: Implementation of a Diagnostic Test and Characterization of the First COVID-19 Cases in the South Island
Rapid Response to SARS-CoV-2 in Aotearoa New Zealand: Implementation of a Diagnostic Test and Characterization of the First COVID-19 Cases in the South Island Open
It has been 20 months since we first heard of SARS-CoV-2, the novel coronavirus detected in the Hubei province, China, in December 2019, responsible for the ongoing COVID-19 pandemic. Since then, a myriad of studies aimed at understanding …
View article: A workflow for simultaneous detection of coding and non-coding transcripts by ribosomal RNA-depleted RNA-Seq
A workflow for simultaneous detection of coding and non-coding transcripts by ribosomal RNA-depleted RNA-Seq Open
RNA sequencing offers unprecedented access to the transcriptome. Key to this is the identification and quantification of many different species of RNA from the same sample at the same time. In this study we describe a novel protocol for si…