James D. Benson
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View article: High-Throughput Evaluation of Cryoprotective Agents for Mixture Effects That Reduce Toxicity
High-Throughput Evaluation of Cryoprotective Agents for Mixture Effects That Reduce Toxicity Open
Vitrification is a promising approach for cryopreserving complex biological structures such as organs. However, to prevent ice formation, high concentrations of cell-permeable cryoprotective agents (CPAs) are required, which can be highly …
View article: High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents
High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents Open
Cryoprotective agent (CPA) toxicity is the most limiting factor impeding cryopreservation of critically needed tissues and organs for transplantation and medical research. This limitation is in part due to the challenge of rapidly screenin…
View article: High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents
High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents Open
Vitrification is the most promising method for cryopreservation of complex structures such as organs and tissue constructs. However, this method requires multimolar concentrations of cell-permeant cryoprotective agents (CPAs), which can be…
View article: Analysis of cryopreservation media thermophysical characteristics after ultra-rapid cooling through differential scanning calorimetry
Analysis of cryopreservation media thermophysical characteristics after ultra-rapid cooling through differential scanning calorimetry Open
Cryoprotective agents play a critical role in minimizing cell damage caused by ice formation during cryopreservation. However, high concentrations of CPAs are toxic to cells and tissues. Required concentrations of CPAs can be reduced by ut…
View article: A three-dimensional lattice-free agent-based model of intracellular ice formation and propagation and intercellular mechanics in liver tissues
A three-dimensional lattice-free agent-based model of intracellular ice formation and propagation and intercellular mechanics in liver tissues Open
A successful cryopreservation of tissues and organs is crucial for medical procedures and drug development acceleration. However, there are only a few instances of successful tissue cryopreservation. One of the main obstacles to successful…
View article: Multiscale transport and 4D time-lapse imaging in precision-cut liver slices (PCLS)
Multiscale transport and 4D time-lapse imaging in precision-cut liver slices (PCLS) Open
Background Monitoring cellular processes across different levels of complexity, from the cellular to the tissue scale, is important for understanding tissue structure and function. However, it is challenging to monitor and estimate these s…
View article: Casing Design for 13000' test gas well in Dripping Rock field near Wamsutter, WY
Casing Design for 13000' test gas well in Dripping Rock field near Wamsutter, WY Open
Background: Casing a wellbore is an integral part of drilling and completing oil and gas wells. In this project, a 13,000 foot test gas well in the Dripping Rock field near Wamsutter, Wyoming was used as a model for the design project. Eco…
View article: Peer Review #1 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)"
Peer Review #1 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)" Open
Sea urchins (e.g.Paracentrotus lividus) are important for both aquaculture and as model species.Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible.Optimized cryoprotectant loading may enabl…
View article: Peer Review #2 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.2)"
Peer Review #2 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.2)" Open
Sea urchins (e.g.Paracentrotus lividus) are important for both aquaculture and as model species.Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible.Optimized cryoprotectant loading may enabl…
View article: Peer Review #3 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)"
Peer Review #3 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)" Open
Sea urchins (e.g.Paracentrotus lividus) are important for both aquaculture and as model species.Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible.Optimized cryoprotectant loading may enabl…
View article: Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( <i>Paracentrotus lividus</i> ) oocytes
Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin ( <i>Paracentrotus lividus</i> ) oocytes Open
Sea urchins ( e.g. , Paracentrotus lividus ) are important for both aquaculture and as model species. Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible. Optimized cryoprotectant loading ma…
View article: Peer Review #2 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)"
Peer Review #2 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.1)" Open
Sea urchins (e.g.Paracentrotus lividus) are important for both aquaculture and as model species.Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible.Optimized cryoprotectant loading may enabl…
View article: Peer Review #1 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.2)"
Peer Review #1 of "Rational synthesis of total damage during cryoprotectant equilibration: modelling and experimental validation of osmomechanical, temperature, and cytotoxic damage in sea urchin (Paracentrotus lividus) oocytes (v0.2)" Open
Sea urchins (e.g.Paracentrotus lividus) are important for both aquaculture and as model species.Despite their importance, biobanking of urchin oocytes by cryopreservation is currently not possible.Optimized cryoprotectant loading may enabl…
View article: Major Histocompatibility Complex-B haplotype and ovarian graft response
Major Histocompatibility Complex-B haplotype and ovarian graft response Open
Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it.…
View article: Technologies for Vitrification Based Cryopreservation
Technologies for Vitrification Based Cryopreservation Open
Cryopreservation is a unique and practical method to facilitate extended access to biological materials. Because of this, cryopreservation of cells, tissues, and organs is essential to modern medical science, including cancer cell therapy,…
View article: Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches
Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches Open
Cryo-injury reduces post-thaw semen quality. Extender components play a protective role, but existing experimental approaches do not elucidate interactions among extender components, semen samples, and post-thaw quality. To identify optima…
View article: Machine learning and hypothesis driven optimization of bull semen cryopreservation media
Machine learning and hypothesis driven optimization of bull semen cryopreservation media Open
Cryopreservation provides a critical tool for dairy herd genetics management. Due to widely varying inter- and within-bull post thaw fertility, recent research on cryoprotectant extender medium has not dramatically improved suboptimal post…
View article: Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies
Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies Open
Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. Cryopreservation has been available for over a century, yet, using current methods, only around 50% of…
View article: Machine learning and hypothesis driven optimization of bull semen cryopreservation media
Machine learning and hypothesis driven optimization of bull semen cryopreservation media Open
Cryopreservation provides a critical tool for dairy herd genetics management. Due to widely varying inter- and within-bull post thaw fertility, recent research on cryoprotectant extender medium has not dramatically improved suboptimal post…
View article: Rational synthesis of total damage during vitrification: modelling and experimental validation of osmotic, temperature, and cytotoxic damage in sea urchin (<i>Paracentrotus lividus)</i> oocytes
Rational synthesis of total damage during vitrification: modelling and experimental validation of osmotic, temperature, and cytotoxic damage in sea urchin (<i>Paracentrotus lividus)</i> oocytes Open
Sea urchin ( Paracentrotus lividus ) oocytes are an important species for aquaculture and as a model species for multiple scientific fields. Despite their importance, methods of cryopreserved biobanking of oocytes are currently not possibl…
View article: Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells
Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells Open
Ovarian tissue cryopreservation transplantation (OTCT) is the most flexible option to preserve fertility in women and children with cancer. However, OTCT is associated with follicle loss and an accompanying short lifespan of the grafts. Cr…
View article: An agent based model of intracellular ice formation and propagation in small tissues
An agent based model of intracellular ice formation and propagation in small tissues Open
Successful cryopreservation of tissues and organs would be a critical tool to accelerate drug discovery and facilitate myriad life saving and quality of life improving medical interventions. Unfortunately success in tissue cryopreservation…