James C. Samuelson
YOU?
Author Swipe
View article: Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencing
Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencing Open
Gene expression is regulated by chromatin DNA methylation and other features, including histone post-translational modifications (PTMs), chromatin remodelers and transcription factor occupancy. A complete understanding of gene regulation w…
View article: Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation andgives new insights into mannose-6-phosphate pathway in cancer
Comparative site-specific N-glycoproteome analysis reveals aberrant N-glycosylation andgives new insights into mannose-6-phosphate pathway in cancer Open
N-glycosylation is implicated in cancers and aberrant N-glycosylation is recognized as a hallmark of cancer. Here, we mapped and compared the site-specific N-glycoproteomes of colon cancer HCT116 cells and isogenic non-tumorigenic DNMT1/3b…
View article: Comparative analyses of the N-glycoproteomes in HCT116 cancer cells and their non-tumorigenic <i>DNMT1</i>/<i>3b</i> double knockout (DKO1) cells and insight into the Mannose-6-phosphate pathway
Comparative analyses of the N-glycoproteomes in HCT116 cancer cells and their non-tumorigenic <i>DNMT1</i>/<i>3b</i> double knockout (DKO1) cells and insight into the Mannose-6-phosphate pathway Open
N-glycoproteomic analyses provide valuable resources for investigation of cancer mechanisms, biomarkers, and therapeutic targets. Here, we mapped and compared the site-specific N-glycoproteomes of colon cancer HCT116 cells and isogenic non…
View article: Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA
Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA Open
Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing data. To overcome these problems, enzymatic methyl-seq (EM-seq) …
View article: Improving the Study of Protein Glycosylation with New Tools for Glycopeptide Enrichment
Improving the Study of Protein Glycosylation with New Tools for Glycopeptide Enrichment Open
High confidence methods are needed for determining the glycosylation profiles of complex biological samples as well as recombinant therapeutic proteins. A common glycan analysis workflow involves liberation of N-glycans from glycoproteins …
View article: EM-seq: Detection of DNA Methylation at Single Base Resolution from Picograms of DNA
EM-seq: Detection of DNA Methylation at Single Base Resolution from Picograms of DNA Open
Bisulfite sequencing is widely used to detect 5mC and 5hmC at single base resolution. However, bisulfite treatment damages DNA resulting in fragmentation, loss of DNA and biased sequencing data. To overcome this, we developed Enzymatic Met…
View article: Leveraging modern DNA assembly techniques for rapid, markerless genome modification
Leveraging modern DNA assembly techniques for rapid, markerless genome modification Open
The ability to alter the genomic material of a prokaryotic cell is necessary for experiments designed to define the biology of the organism. In addition, the production of biomolecules may be significantly improved by application of engine…