James D. Higgins
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View article: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
SCEP3 initiates synapsis and implements crossover interference in Arabidopsis Open
The synaptonemal complex (SC) is a meiosis-specific tripartite proteinaceous structure that regulates the number and positions of crossovers (COs). Here we characterize SCEP3, a new Arabidopsis SC component that is essential for CO assuran…
View article: An overview of recent advances on wheat homologous and homoeologous recombination
An overview of recent advances on wheat homologous and homoeologous recombination Open
Crop breeding relies on the creation of high-performance varieties that possess a genotype containing numerous agronomical traits of interest. The specific combination of traits is generated in the parents by meiotic recombination, a highl…
View article: CONTROLLING HOMOLOGOUS AND HOMOEOLOGOUS RECOMBINATION IN BREAD WHEAT TO ENHANCE THE DEVELOPMENT OF ORIGINAL AND PRODUCTIVE WHEAT VARIETIES
CONTROLLING HOMOLOGOUS AND HOMOEOLOGOUS RECOMBINATION IN BREAD WHEAT TO ENHANCE THE DEVELOPMENT OF ORIGINAL AND PRODUCTIVE WHEAT VARIETIES Open
International audience
View article: Kinetochore and ionomic adaptation to whole-genome duplication in Cochlearia shows evolutionary convergence in three autopolyploids
Kinetochore and ionomic adaptation to whole-genome duplication in Cochlearia shows evolutionary convergence in three autopolyploids Open
Whole-genome duplication (WGD) occurs in all kingdoms and impacts speciation, domestication, and cancer outcome. However, doubled DNA management can be challenging for nascent polyploids. The study of within-species polyploidy (autopolyplo…
View article: <scp>FIGL1</scp> prevents aberrant chromosome associations and fragmentation and limits crossovers in polyploid wheat meiosis
<span>FIGL1</span> prevents aberrant chromosome associations and fragmentation and limits crossovers in polyploid wheat meiosis Open
Summary Meiotic crossovers (COs) generate genetic diversity and are crucial for viable gamete production. Plant COs are typically limited to 1–3 per chromosome pair, constraining the development of improved varieties, which in wheat is exa…
View article: MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism
MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism Open
Meiotic crossovers can be formed through the interfering pathway, in which one crossover prevents another from forming nearby, or by an independent non-interfering pathway. In Arabidopsis, local sequence polymorphism between homologs can s…
View article: Kinetochore and ionomic adaptation to whole genome duplication
Kinetochore and ionomic adaptation to whole genome duplication Open
Transforming genomic and cellular landscapes in a single generation, whole genome duplication (WGD) brings fundamental challenges, but is also associated with diversification. How is WGD tolerated, and what processes commonly evolve to sta…
View article: ASYNAPSIS 1 ensures crossover fidelity in polyploid wheat by promoting homologous recombination and suppressing non-homologous recombination
ASYNAPSIS 1 ensures crossover fidelity in polyploid wheat by promoting homologous recombination and suppressing non-homologous recombination Open
During meiosis, the chromosome axes and synaptonemal complex mediate chromosome pairing and homologous recombination to maintain genomic stability and accurate chromosome segregation. In plants, ASYNAPSIS 1 (ASY1) is a key component of the…
View article: MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism
MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism Open
In Arabidopsis, local sequence polymorphism between homologs can stimulate double-strand break (DSB) repair via crossover formation in a MSH2-dependent manner. To understand how MSH2 regulates crossovers formed by the independent interferi…
View article: The coordinated regulation of early meiotic stages is dominated by non‐coding <scp>RNAs</scp> and stage‐specific transcription in wheat
The coordinated regulation of early meiotic stages is dominated by non‐coding <span>RNAs</span> and stage‐specific transcription in wheat Open
SUMMARY Reproductive success hinges on precisely coordinated meiosis, yet our understanding of how structural rearrangements of chromatin and phase transitions during meiosis are transcriptionally regulated is limited. In crop plants, deta…
View article: Identification, characterization, and rescue of <scp>CRISPR</scp>/Cas9 generated wheat <i>SPO11‐1</i> mutants
Identification, characterization, and rescue of <span>CRISPR</span>/Cas9 generated wheat <i>SPO11‐1</i> mutants Open
Summary Increasing crop yields through plant breeding is time consuming and laborious, with the generation of novel combinations of alleles being limited by chromosomal linkage blocks and linkage‐drag. Meiotic recombination is essential to…
View article: Unravelling mechanisms that govern meiotic crossover formation in wheat
Unravelling mechanisms that govern meiotic crossover formation in wheat Open
Wheat is a major cereal crop that possesses a large allopolyploid genome formed through hybridisation of tetraploid and diploid progenitors. During meiosis, crossovers (COs) are constrained in number to 1–3 per chromosome pair that are pre…
View article: Sporophytic control of pollen meiotic progression is mediated by tapetum expression of <i>ABORTED MICROSPORES</i>
Sporophytic control of pollen meiotic progression is mediated by tapetum expression of <i>ABORTED MICROSPORES</i> Open
Pollen development is dependent on the tapetum, a sporophytic anther cell layer surrounding the microspores that functions in pollen wall formation but is also essential for meiosis-associated development. There is clear evidence of crosst…
View article: Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation
Crossover-active regions of the wheat genome are distinguished by DMC1, the chromosome axis, H3K27me3, and signatures of adaptation Open
The hexaploid bread wheat genome comprises over 16 gigabases of sequence across 21 chromosomes. Meiotic crossovers are highly polarized along the chromosomes, with elevation in the gene-dense distal regions and suppression in the Gypsy ret…
View article: Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters
Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters Open
PRDM9 directs human meiotic crossover hotspots to intergenic sequence motifs, whereas budding yeast hotspots overlap low nucleosome density regions in gene promoters. To investigate hotspots in plants, which lack PRDM9, we used coalescent …
View article: ZYP1 is required for obligate cross-over formation and cross-over interference in<i>Arabidopsis</i>
ZYP1 is required for obligate cross-over formation and cross-over interference in<i>Arabidopsis</i> Open
Significance The synaptonemal complex (SC) is a meiosis-specific proteinaceous ultrastructure required to ensure cross-over (CO) formation in the majority of sexually reproducing eukaryotes. It is composed of two lateral elements adjoined …
View article: Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program
Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program Open
Meiotic recombination generates genetic variation and provides physical links between homologous chromosomes (crossovers) essential for accurate segregation. In cereals the distribution of crossovers, cytologically evident as chiasmata, is…
View article: Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells
Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells Open
The mammalian BREAST CANCER 2 (BRCA2) gene is a tumor suppressor that plays a crucial role in DNA repair and homologous recombination (HR). Here, we report the identification and characterization of OsBRCA2, the rice orthologue of human BR…
View article: Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers
Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers Open
Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has re…
View article: <scp>MSH</scp> 2 shapes the meiotic crossover landscape in relation to interhomolog polymorphism in Arabidopsis
<span>MSH</span> 2 shapes the meiotic crossover landscape in relation to interhomolog polymorphism in Arabidopsis Open
During meiosis, DNA double-strand breaks undergo interhomolog repair to yield crossovers between homologous chromosomes. To investigate how interhomolog sequence polymorphism affects crossovers, we sequenced multiple recombinant population…
View article: Genotype and phenotype data.
Genotype and phenotype data. Open
Diploid lyrata alleles = ly; diploid arenosa alleles = ar and ar/ly = diploid arenosa to diploid lyrata putative gene conversions. (DOCX)
View article: A novel allele of ASY3 is associated with greater meiotic stability in autotetraploid Arabidopsis lyrata
A novel allele of ASY3 is associated with greater meiotic stability in autotetraploid Arabidopsis lyrata Open
In this study we performed a genotype-phenotype association analysis of meiotic stability in 10 autotetraploid Arabidopsis lyrata and A. lyrata/A. arenosa hybrid populations collected from the Wachau region and East Austrian Forealps. The …
View article: <i>MutS homologue 4</i> and <i>MutS homologue 5</i> Maintain the Obligate Crossover in Wheat Despite Stepwise Gene Loss following Polyploidization
<i>MutS homologue 4</i> and <i>MutS homologue 5</i> Maintain the Obligate Crossover in Wheat Despite Stepwise Gene Loss following Polyploidization Open
Crossovers (COs) ensure accurate chromosome segregation during meiosis while creating novel allelic combinations. Here, we show that allotetraploid (AABB) durum wheat (Triticum turgidum ssp. durum) utilizes two pathways of meiotic recombin…
View article: Analysis of meiotic segregation by triple-color fish on both total and motile sperm fractions in a t(1p;18) river buffalo bull
Analysis of meiotic segregation by triple-color fish on both total and motile sperm fractions in a t(1p;18) river buffalo bull Open
Chromosomal aberrations are relatively frequent pathologies in both humans and animals. Among them, translocations present a specific meiotic segregation pattern able to give a higher percentage of unbalanced gametes that can induce fertil…
View article: Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis
Interacting Genomic Landscapes of REC8-Cohesin, Chromatin, and Meiotic Recombination in Arabidopsis Open
Meiosis recombines genetic variation and influences eukaryote genome evolution. During meiosis, DNA double-strand breaks (DSBs) enter interhomolog repair to yield crossovers and noncrossovers. DSB repair occurs as replicated sister chromat…
View article: A novel allele of<i>ASY3</i>promotes meiotic stability in autotetraploid<i>Arabidopsis lyrata</i>
A novel allele of<i>ASY3</i>promotes meiotic stability in autotetraploid<i>Arabidopsis lyrata</i> Open
In this study we performed a genotype-phenotype association analysis of meiotic stability in ten autotetraploid Arabidopsis lyrata and A . lyrata/A. arenosa hybrid populations collected from the Wachau region and East Austrian Forealps. Th…