James P. Di Santo
YOU?
Author Swipe
View article: Tracking HIV-1 DNA fate from Cell Culture to Humanized mice Tissues
Tracking HIV-1 DNA fate from Cell Culture to Humanized mice Tissues Open
Unambiguous identification of HIV reservoirs is essential for their characterization and for developing curative strategies. A major obstacle to curing HIV is the persistence of silent viral genomes in host cells that evade immune detectio…
View article: Investigation of the sero-epidemiology of vaccine preventable diseases and common viral infections in French populations
Investigation of the sero-epidemiology of vaccine preventable diseases and common viral infections in French populations Open
These findings highlight the potential of high-throughput multiplex serology assays for routine surveillance of common viral infections and assessment of vaccine coverage.
View article: The influence of environment on bacterial co-abundance in the gut microbiomes of healthy human individuals
The influence of environment on bacterial co-abundance in the gut microbiomes of healthy human individuals Open
View article: Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria
Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria Open
View article: Adaptive immune responses to SARS-CoV-2 in DMARD-treated patients with chronic inflammatory rheumatisms
Adaptive immune responses to SARS-CoV-2 in DMARD-treated patients with chronic inflammatory rheumatisms Open
Background Patients with rheumatoid arthritis (RA) and spondyloarthritis (SpA) are at an increased risk for infection related to the use of immunomodulatory therapies (ITs). The objective of this study is to assess the impact of ITs on the…
View article: Intratumoral delivery of FLT3L with CXCR3/CCR5 ligands promotes XCR1 <sup>+</sup> cDC1 infiltration and activates anti-tumor immunity
Intratumoral delivery of FLT3L with CXCR3/CCR5 ligands promotes XCR1 <sup>+</sup> cDC1 infiltration and activates anti-tumor immunity Open
Tumor infiltration by XCR1⁺ conventional dendritic cells (cDC1) correlates strongly with favorable prognosis and improved responses to immunotherapy. Yet, tumor-driven immunosuppressive programs restrict efficient cDC1 recruitment, highlig…
View article: Modeling viral and bacterial infections in human lung organotypic systems reveals strain specific host responses
Modeling viral and bacterial infections in human lung organotypic systems reveals strain specific host responses Open
In this study, we developed novel lung organoid-on-chip models that elucidate differential human tissue response to various strains of respiratory pathogens: Streptococcus pneumoniae and SARS-CoV-2. We show that human fetal-derived distal …
View article: The Healthy Human Global Project - Hong Kong: A Community-Based Cross-Sectional Study of a Healthy Asian Population
The Healthy Human Global Project - Hong Kong: A Community-Based Cross-Sectional Study of a Healthy Asian Population Open
View article: In vivo HIV-1 nuclear condensates safeguard against cGAS and license reverse transcription
In vivo HIV-1 nuclear condensates safeguard against cGAS and license reverse transcription Open
View article: IL-7–dependent and –independent lineages of IL-7R–dependent human T cells
IL-7–dependent and –independent lineages of IL-7R–dependent human T cells Open
Infants with biallelic IL7R loss-of-function variants have severe combined immune deficiency (SCID) characterized by the absence of autologous T lymphocytes, but normal counts of circulating B and NK cells (T-B+NK+ SCID). We report 6 adult…
View article: Smoking changes adaptive immunity with persistent effects
Smoking changes adaptive immunity with persistent effects Open
View article: Divergent roles for STAT4 in shaping differentiation of cytotoxic ILC1 and NK cells during gut inflammation
Divergent roles for STAT4 in shaping differentiation of cytotoxic ILC1 and NK cells during gut inflammation Open
Natural killer (NK) cells and type 1 innate lymphoid cells (ILC1) require signal transducer and activator of transcription 4 (STAT4) to elicit rapid effector responses and protect against pathogens. By combining genetic and transcriptomic …
View article: Standardized high‐dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies
Standardized high‐dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies Open
Flow cytometry is the method of choice for immunophenotyping in the context of clinical, translational, and systems immunology studies. Among the latter, the Milieu Intérieur (MI) project aims at defining the boundaries of a healthy immune…
View article: A human immune system (HIS) mouse model that dissociates roles for mouse and human FcR<sup>+</sup> cells during antibody‐mediated immune responses
A human immune system (HIS) mouse model that dissociates roles for mouse and human FcR<sup>+</sup> cells during antibody‐mediated immune responses Open
Human immune system (HIS) mice provide a model to study human immune responses in vivo. Currently available HIS mouse models may harbor mouse Fc Receptor (FcR)‐expressing cells that exert potent effector functions following administration …
View article: High-dimensional spectral cytometry panels for whole blood immune phenotyping
High-dimensional spectral cytometry panels for whole blood immune phenotyping Open
The need to understand the mechanisms and pathways of immune responses in pathogenic conditions such as cancer and autoimmunity requires awareness of natural immune variability in healthy subjects. To this end, various systems immunology s…
View article: Integrated analysis of whole blood oxylipin and cytokine responses after bacterial, viral, and T cell stimulation reveals new immune networks
Integrated analysis of whole blood oxylipin and cytokine responses after bacterial, viral, and T cell stimulation reveals new immune networks Open
View article: Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria
Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria Open
View article: The immune factors driving DNA methylation variation in human blood
The immune factors driving DNA methylation variation in human blood Open
View article: Inflammation triggers ILC3 patrolling of the intestinal barrier
Inflammation triggers ILC3 patrolling of the intestinal barrier Open
View article: Notch, RORC and IL-23 signals cooperate to promote multi-lineage human innate lymphoid cell differentiation
Notch, RORC and IL-23 signals cooperate to promote multi-lineage human innate lymphoid cell differentiation Open
View article: Early IFNβ secretion determines variable downstream IL-12p70 responses upon TLR4 activation
Early IFNβ secretion determines variable downstream IL-12p70 responses upon TLR4 activation Open
View article: Rhesus negative males have an enhanced IFNγ-mediated immune response to influenza A virus
Rhesus negative males have an enhanced IFNγ-mediated immune response to influenza A virus Open
The Rhesus D antigen (RhD) has been associated with susceptibility to several viral infections. Reports suggest that RhD-negative individuals are better protected against infectious diseases and have overall better health. However, potenti…
View article: Integrative genetic and immune cell analysis of plasma proteins in healthy donors identifies novel associations involving primary immune deficiency genes
Integrative genetic and immune cell analysis of plasma proteins in healthy donors identifies novel associations involving primary immune deficiency genes Open
View article: Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller
Epitope convergence of broadly HIV-1 neutralizing IgA and IgG antibody lineages in a viremic controller Open
Decrypting the B cell ontogeny of HIV-1 broadly neutralizing antibodies (bNAbs) is paramount for vaccine design. Here, we characterized IgA and IgG bNAbs of three distinct B cell lineages in a viremic controller, two of which comprised onl…
View article: Trained ILC3 responses promote intestinal defense
Trained ILC3 responses promote intestinal defense Open
Group 3 innate lymphoid cells (ILC3s) are innate immune effectors that contribute to host defense. Whether ILC3 functions are stably modified after pathogen encounter is unknown. Here, we assess the impact of a time-restricted enterobacter…
View article: Defects in mucosal immunity and nasopharyngeal dysbiosis in HSC-transplanted SCID patients with IL2RG/JAK3 deficiency
Defects in mucosal immunity and nasopharyngeal dysbiosis in HSC-transplanted SCID patients with IL2RG/JAK3 deficiency Open
Both innate and adaptive lymphocytes have critical roles in mucosal defense that contain commensal microbial communities and protect against pathogen invasion. Here we characterize mucosal immunity in patients with severe combined immunode…
View article: CD116+ fetal precursors migrate to the perinatal lung and give rise to human alveolar macrophages
CD116+ fetal precursors migrate to the perinatal lung and give rise to human alveolar macrophages Open
Despite their importance in lung health and disease, it remains unknown how human alveolar macrophages develop early in life. Here we define the ontogeny of human alveolar macrophages from embryonic progenitors in vivo, using a humanized m…
View article: Our latest edition of Flow Cytometry Guidelines for basic and clinical immunologists!
Our latest edition of Flow Cytometry Guidelines for basic and clinical immunologists! Open
Dear readers, The last issue of 2021 of the European Journal of Immunology (EJI) is special in that it features, among other article types, the third edition of the ‘Guidelines for the use of flow cytometry and cell sorting in immunologica…
View article: Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) Open
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine im…
View article: Étude de la réponse humorale anti-SARS-CoV-2 et de la persistance virale dans une cohorte de 96 patients avec rhumatisme inflammatoire chronique (PR et SpA) sous immunomodulateurs, infectés par le SARS-CoV-2
Étude de la réponse humorale anti-SARS-CoV-2 et de la persistance virale dans une cohorte de 96 patients avec rhumatisme inflammatoire chronique (PR et SpA) sous immunomodulateurs, infectés par le SARS-CoV-2 Open