James E. Bear
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View article: Optogenetic control of PLC-γ1 activity polarizes cell motility
Optogenetic control of PLC-γ1 activity polarizes cell motility Open
Phospholipase C-γ1 (PLC-γ1) signaling is required for mesenchymal chemotaxis, but is it sufficient to bias motility? PLC-γ1 enzyme activity is basally autoinhibited, and light-controlled membrane recruitment of wild-type (WT) PLC-γ1 (OptoP…
View article: A Photopolymerized Hydrogel System with Dual Stiffness Gradients Reveals Distinct Actomyosin-Based Mechano-Responses in Fibroblast Durotaxis
A Photopolymerized Hydrogel System with Dual Stiffness Gradients Reveals Distinct Actomyosin-Based Mechano-Responses in Fibroblast Durotaxis Open
Durotaxis, migration of cells directed by a stiffness gradient, is critical in development and disease. To distinguish durotaxis-specific migration mechanisms from those on uniform substrate stiffnesses, we engineered an all-in-one photopo…
View article: The NHSL1-A complex interacts with the Arp2/3 complex and controls cell migration efficiency and chemotaxis
The NHSL1-A complex interacts with the Arp2/3 complex and controls cell migration efficiency and chemotaxis Open
Cell migration is crucial for development and deregulation causes diseases. The Scar/WAVE complex promotes mesenchymal cell migration through Arp2/3 mediated lamellipodia protrusion. We previously discovered that all isoforms of Nance-Hora…
View article: Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction
Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction Open
The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical necessity of the nucleus …
View article: Measurement of cellular traction forces during confined migration
Measurement of cellular traction forces during confined migration Open
To migrate efficiently through tissues, cells must transit through small constrictions within the extracellular matrix. However, in vivo environments are geometrically, mechanically, and chemically complex, and it has been difficult to und…
View article: An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation
An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation Open
Histone H3 lysine 36 methylation (H3K36me) is a conserved histone modification associated with transcription and DNA repair. Although the effects of H3K36 methylation have been studied, the genome-wide dynamics of H3K36me deposition and re…
View article: Prolonged depletion of profilin 1 or F-actin causes an adaptive response in microtubules
Prolonged depletion of profilin 1 or F-actin causes an adaptive response in microtubules Open
In addition to its well-established role in actin assembly, profilin 1 (PFN1) has been shown to bind to tubulin and alter microtubule growth. However, whether PFN1’s predominant control over microtubules in cells occurs through direct regu…
View article: Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly
Local monomer levels and established filaments potentiate non-muscle myosin 2 assembly Open
The ability to dynamically assemble contractile networks is required throughout cell physiology, yet direct biophysical mechanisms regulating non-muscle myosin 2 filament assembly in living cells are lacking. Here, we use a suite of dynami…
View article: Viral airway injury promotes cell engraftment in an in vitro model of cystic fibrosis cell therapy
Viral airway injury promotes cell engraftment in an in vitro model of cystic fibrosis cell therapy Open
Cell therapy is a potential treatment for cystic fibrosis (CF). Here, we model cell engraftment by injuring CF air–liquid interface cultures and delivering non-CF cells. Successful engraftment required severe epithelial injury. Intentional…
View article: Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes
Cytoskeletal adaptation following long-term dysregulation of actomyosin in neuronal processes Open
Microtubules, intermediate filaments, and actin are cytoskeletal polymer networks found within the cell. While each has unique functions, all the cytoskeletal elements must work together for cellular mechanics to be fully operative. This i…
View article: The actin binding protein profilin 1 is critical for mitochondria function
The actin binding protein profilin 1 is critical for mitochondria function Open
Profilin 1 (PFN1) is an actin binding protein that is vital for the polymerization of monomeric actin into filaments. Here we screened knockout cells for novel functions of PFN1 and discovered that mitophagy, a type of selective autophagy …
View article: Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly
Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly Open
The ability to dynamically assemble contractile networks is required throughout cell physiology, yet the biophysical mechanisms regulating non-muscle myosin 2 filament assembly in living cells are lacking. Here we use a suite of dynamic, q…
View article: Supplementary Figure S4 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S4 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S4. Deletion of LERER in MenaINV affects tumor growth in a xenograft model.
View article: Supplementary Figure S3 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S3 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S3. TCGA analysis supplementary analysis and characterization of MenaINV-specific monoclonal antibody.
View article: Supplementary Figure S3 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S3 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S3. TCGA analysis supplementary analysis and characterization of MenaINV-specific monoclonal antibody.
View article: Supplementary Figure S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S1. Effects on haptotaxis are independent of effects on cell velocity.
View article: Supplementary Figure S5 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S5 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S5. Mena isoform expression does not affect Integrin alphav, beta1 or FAK levels.
View article: Data from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Data from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Fibronectin (FN) is a major component of the tumor microenvironment, but its role in promoting metastasis is incompletely understood. Here, we show that FN gradients elicit directional movement of breast cancer cells, in vitro and in vivo.…
View article: Supplementary Figure S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S1. Effects on haptotaxis are independent of effects on cell velocity.
View article: Data from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Data from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Fibronectin (FN) is a major component of the tumor microenvironment, but its role in promoting metastasis is incompletely understood. Here, we show that FN gradients elicit directional movement of breast cancer cells, in vitro and in vivo.…
View article: Supplementary Figure S2 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S2 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S2. MenaINV drives haptotaxis at high FN concentrations.
View article: Supplementary Figure S4 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S4 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S4. Deletion of LERER in MenaINV affects tumor growth in a xenograft model.
View article: Supplementary Figure Legends from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure Legends from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure Legends
View article: Supplementary Figure S6 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S6 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S6. MenaINV-driven ECM reorganization in vitro and in vivo.
View article: Supplementary Video S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Video S1 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Video S1. MenaINV drives haptotaxis in vivo.
View article: Supplementary Figure S6 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression
Supplementary Figure S6 from Tumor Cell–Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression Open
Supplementary Figure S6. MenaINV-driven ECM reorganization in vitro and in vivo.