James E. Hudson
YOU?
Author Swipe
View article: Endothelial specialization and angiocrine factors are critical for vascularized organoid models
Endothelial specialization and angiocrine factors are critical for vascularized organoid models Open
A vast network of vessels and capillaries is required in most organs because effective diffusion is typically limited to less than 200 μm. Most tissue engineering efforts have focused on re-creating vascularized networks to overcome this s…
View article: Using modified mRNA for cardiomyocyte proliferation and cardiac genetic disease modelling and treatment
Using modified mRNA for cardiomyocyte proliferation and cardiac genetic disease modelling and treatment Open
Heart failure (HF) is a leading cause of death worldwide and the associated mortality and socioeconomic burden is predicted to worsen. Current therapies for HF focus on managing the causes and symptoms; however, these current treatment opt…
View article: Contractile function maintains cardiomyocyte differentiation and inhibits cell cycle activity
Contractile function maintains cardiomyocyte differentiation and inhibits cell cycle activity Open
Numerous endotherm species lose cardiac regenerative capacity shortly after birth, which is in contrast to many ectotherm species who regenerate throughout life. Whether the enhanced contractile function required for endothermy contributes…
View article: Alpha Protein Kinase 3 Gene Therapy Restores Heart Function in Mouse and Human Models of Cardiomyopathy
Alpha Protein Kinase 3 Gene Therapy Restores Heart Function in Mouse and Human Models of Cardiomyopathy Open
Truncating variants in the Alpha Kinase 3 (ALPK3) gene have recently emerged as an important cause of genetic cardiomyopathy globally and here we demonstrate the efficacy and safety of a viral-based gene replacement therapy for ALPK3. Give…
View article: Designing multicellular cardiac tissue engineering technologies for clinical translation
Designing multicellular cardiac tissue engineering technologies for clinical translation Open
Cardiovascular diseases remain the leading cause of death worldwide-claiming one-third of all deaths every year. Current two-dimensional in vitro cell culture systems and animal models cannot completely recapitulate the clinical complexity…
View article: Muscle-specific Ryanodine receptor 1 properties underlie limb-girdle muscular dystrophy 2B/R2 progression
Muscle-specific Ryanodine receptor 1 properties underlie limb-girdle muscular dystrophy 2B/R2 progression Open
Ryanodine receptor 1 Ca2+ leak is a signal in skeletal muscle, but chronic leak can underlie pathology. Here we show that in healthy male mouse, limb-girdle muscle presents higher sympathetic input, elevated ryanodine receptor 1 basal phos…
View article: Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates
Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates Open
Polydimethylsiloxane (PDMS) is a commonly used material for the design of new cell culture devices. Typically, molded PDMS is bonded to glass or another PDMS layer; however, it is challenging to form high-strength bonds with polymers commo…
View article: Targeted glycophagy ATG8 therapy reverses diabetic heart disease in mice and in human engineered cardiac tissues
Targeted glycophagy ATG8 therapy reverses diabetic heart disease in mice and in human engineered cardiac tissues Open
Diabetic heart disease is highly prevalent and is associated with the early development of impaired diastolic relaxation. The mechanisms of diabetic heart disease are poorly understood and it is a condition for which there are no targeted …
View article: A community-oriented, data-driven resource to improve protocol design for cardiac modelling from human pluripotent stem cells
A community-oriented, data-driven resource to improve protocol design for cardiac modelling from human pluripotent stem cells Open
Protocols for culturing cardiac cells derived from human-induced pluripotent stem cells (hiPSCs) are critical for studying mechanisms of development and disease. This study provides data-driven analysis of a community-oriented database to …
View article: Maturation of human cardiac organoids enables complex disease modelling and drug discovery
Maturation of human cardiac organoids enables complex disease modelling and drug discovery Open
Summary Cardiac maturation is an important developmental phase culminating in profound biological and functional changes to adapt to the high demand environment after birth 1,2 . Maturation of human pluripotent stem cell-derived human card…
View article: CMPortal: a community-oriented, data-driven resource to inform protocol design for cardiac modelling from human pluripotent stem cells
CMPortal: a community-oriented, data-driven resource to inform protocol design for cardiac modelling from human pluripotent stem cells Open
Protocol design and benchmarking is central to optimising model development using human pluripotent stem cell derived cardiomyocytes (hPSC-CMs). By applying data mining to decades of research and hundreds of peer reviewed studies, we evalu…
View article: A miniaturized culture platform for control of the metabolic environment
A miniaturized culture platform for control of the metabolic environment Open
The heart is a metabolic “omnivore” and adjusts its energy source depending on the circulating metabolites. Human cardiac organoids, a three-dimensional in vitro model of the heart wall, are a useful tool to study cardiac physiology and pa…
View article: Pharmacological or genetic inhibition of LTCC promotes cardiomyocyte proliferation through inhibition of calcineurin activity.
Pharmacological or genetic inhibition of LTCC promotes cardiomyocyte proliferation through inhibition of calcineurin activity. Open
Cardiomyocytes (CMs) lost during ischemic cardiac injury cannot be replaced due to their limited proliferative capacity, which leads to progressive heart failure. Calcium (Ca2+) is an important signal transducer that regulates key cellular…
View article: Regulation of Non-Canonical Proteins Encoded by Small Open Reading Frames via the Nonsense-Mediated Decay Pathway
Regulation of Non-Canonical Proteins Encoded by Small Open Reading Frames via the Nonsense-Mediated Decay Pathway Open
Immunotherapy harnesses neoantigens encoded within the human genome, but their therapeutic potential is hampered by low expression, which may be controlled by the Nonsense-Mediated Decay (NMD) pathway. This study investigates the impact of…
View article: Regulation of Non-Canonical Proteins Encoded by Small Open Reading Frames via the Nonsense-Mediated Decay Pathway
Regulation of Non-Canonical Proteins Encoded by Small Open Reading Frames via the Nonsense-Mediated Decay Pathway Open
Immunotherapy interventions relies heavily on neoantigen availability. The human genome encodes non-canonical/mutant proteins that potentially contain neoantigenic peptides. Nevertheless, their typically low expression, potentially moderat…
View article: GLA-modified RNA treatment lowers GB3 levels in iPSC-derived cardiomyocytes from Fabry-affected individuals
GLA-modified RNA treatment lowers GB3 levels in iPSC-derived cardiomyocytes from Fabry-affected individuals Open
Recent studies in non-human model systems have shown therapeutic potential of nucleoside-modified messenger RNA (modRNA) treatments for lysosomal storage diseases. Here, we assessed the efficacy of a modRNA treatment to restore the express…
View article: <i>Sox7</i> ‐positive endothelial progenitors establish coronary arteries and govern ventricular compaction
<i>Sox7</i> ‐positive endothelial progenitors establish coronary arteries and govern ventricular compaction Open
The cardiac endothelium influences ventricular chamber development by coordinating trabeculation and compaction. However, the endothelial‐specific molecular mechanisms mediating this coordination are not fully understood. Here, we identify…
View article: A transient modified mRNA encoding Myc and Cyclin T1 induces cardiac regeneration and improves cardiac function after myocardial injury
A transient modified mRNA encoding Myc and Cyclin T1 induces cardiac regeneration and improves cardiac function after myocardial injury Open
Cardiac injury, such as myocardial infarction (MI), results in permanent loss of cardiomyocytes and in many cases heart failure. Transgenic expression of the pro-proliferative transcription factor Myc and Cyclin T1 can drive substantial ad…